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Cryo-EM studies reveal that RYBP–PRC1 uses two distinct interfaces for binding unmodified and H2Aub1-modified nucleosomes. These binding modes enable the complex to generate H2Aub1 chromatin domains by a read–write mechanism.
The biogenesis and recycling of the ‘heart’ of the human spliceosome, the U5 small nuclear ribonucleoprotein (snRNP), requires CD2BP2 and TSSC4. Here the authors present cryo-electron microscopy structures that reveal how these protein chaperones orchestrate the ATP-independent (re)generation of the U5 snRNP.
Using deep mutational scanning, the authors uncover the functional consequence of missense mutations in ARID1B, a subunit of the SWI/SNF chromatin remodeling complex that is frequently mutated in human Coffin–Siris syndrome and in cancer.
Structures of complete extracellular receptor assemblies mediated by the pro-inflammatory cytokines IL-12 and IL-23 reveal key commonalities and diverse features, with only IL-12 juxtaposing receptor domains proximal to the cell membrane.
This study revealed the mechanism by which the E3 ligase Bre1 directs monoubiquitination of histone H2B at K123 by the E2 ubiquitin-conjugating enzyme Rad6. Comparison with other dimeric E3 ligases suggests a pivot-like mechanism in which one subunit ‘tunes’ the specificity for particular histone residues.
Here, the authors present droplet-based Paired-Tag, an improved method to concomitantly map histone modifications and gene expression at single-cell resolution. They functionally benchmark the method and demonstrate its advantages in mammalian cells and primary brain tissues.
Here, the authors demonstrate that the translation of the Drosophila transcript of insulin (dilp2) is regulated by methylation of N6-adenosine (m6A) in the 3′ UTR, at sites also conserved in mammals. In turn, this results in aberrant, diabetes-like functional phenotypes.
Here, the authors solve the cryogenic electron microscopy structure of a human primosome to shed light on the mechanism by which RNA–DNA primers are synthesized for the initiation of DNA replication and the structural basis of the primer length limitation.
Gabapentinoid drugs are widely used for pain, epilepsy and mental disorders. Chen et al. report the structure of a gabapentin-bound brain and heart calcium channel, revealing the gabapentin binding site and isoform-selective binding determinants.
Here the authors use cryo-EM and biochemical analysis to show how the ancillary protein TPR-CHAT regulates the nuclease function of the CRISPR-guided nuclease Cas7-11.
A cryo-EM structure of disease-related human Y145Stop prion protein amyloid fibrils explains species-dependent seeding barriers in prion protein amyloid propagation.
Kottur et al. present high-resolution structures of SARS-CoV-2 nsp14 N7-MTase that will aid in the development of new antivirals against this and other pathogenic coronaviruses.
AlphaFold2 predictions, X-ray crystallography and cryo-EM analyses reveal how related human glycoproteins GP2 and uromodulin catch pathogenic bacteria by presenting a high-mannose glycan that acts as a decoy for fimbrial adhesin FimH.
An online and interactive G-protein coupled receptor (GPCR) structure analysis platform allows any researcher to analyze and visualize a plethora of structure–function relationships across the scales of atomic interactions to protein backbone rearrangements.
The cryo-EM structure of DNA-assembled histone pairs Hβ-Hα and Hδ-Hγ from Marseillevirus, a nucleocytoplasmic large DNA virus, reveals that these proteins form viral nucleosomes with highly conserved features when compared to canonical eukaryotic nucleosomes.
The SARS-CoV-2 spike ectodomain is destabilized by cold temperature storage, an effect that can be reversed by incubation at 37 °C or by stabilizing its conformation in the ‘down’ state.
A crystal structure of SARS-CoV-2 with inhibitor carmofur reveals the mechanism of action of this compound and opens the way to develop more potent drugs.
The structure of the second zinc finger of SALL4 in complex with pomalidomide, cereblon and DDB1 reveals the unique details of SALL4 recruitment, providing insights for rational design of cereblon-binding drugs with reduced teratogenic risk.
