Research articles

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  • GPAT1 is a mitochondrial outer membrane protein that catalyzes the first step of glycerolipid biosynthesis. Cryo-EM structures and functional studies of human GPAT1 uncover the molecular architecture and mechanism of this important acyltransferase.

    • Zachary Lee Johnson
    • Mark Ammirati
    • Huixian Wu
    Article
  • The structure of a heteromeric volume-regulated LRRC8A/C channel shows a hexameric assembly of four clustered A subunits interspersed by two C subunits, which increase the mobility of the protein, thus facilitating channel activation.

    • Sonja Rutz
    • Dawid Deneka
    • Raimund Dutzler
    Article Open Access
  • ABC-type heterotrimeric proteins can be designed de novo using coiled coils and helical bundles as starting scaffolds, extended using helical repeat proteins and then used as building blocks for higher-order oligomeric assemblies.

    • Sherry Bermeo
    • Andrew Favor
    • David Baker
    Article Open Access
  • LRRK2 is one of the most commonly mutated genes in familial Parkinson’s disease. Here, the authors report a cryo-EM structure of the catalytic half of LRRK2 bound to microtubules, revealing determinants of binding that are independent of LRRK2 kinase activity.

    • David M. Snead
    • Mariusz Matyszewski
    • Samara L. Reck-Peterson
    Article Open Access
  • The authors perform parallel transcriptome sequencing, tRNA qPCR array, ribosome profiling and mass spectrometry across five stages of mouse neocortex development to model the dynamic transcriptome-to-proteome transition, discovering a critical prenatal window during which translational control is predominant.

    • Dermot Harnett
    • Mateusz C. Ambrozkiewicz
    • Matthew L. Kraushar
    Article Open Access
  • The authors describe how three types of cis-element (CTCF sites, active TSSs and TTSs) regulate cohesin trafficking along chromosomes. They uncover that this cohesin traffic pattern is genetically linked to gene regulation and RNA processing.

    • Anne-Laure Valton
    • Sergey V. Venev
    • Job Dekker
    Article
  • Basu and colleagues comprehensively characterize how sequence and epigenetic modifications impact the local mechanical properties of DNA. The results suggest that DNA mechanics may have evolved to aid diverse DNA-deforming biological processes.

    • Aakash Basu
    • Dmitriy G. Bobrovnikov
    • Taekjip Ha
    Article
  • Here the authors use cryo-EM and biochemical analysis to show how the ancillary protein TPR-CHAT regulates the nuclease function of the CRISPR-guided nuclease Cas7-11.

    • Babatunde Ekundayo
    • Davide Torre
    • Dongchun Ni
    Brief Communication Open Access
  • Hall et al. discovered the molecular mechanism for the polarity of the CRISPR roadblock to transcription: RNA polymerase progression can collapse the R-loop formed by a bound dCas to allow read-through from the PAM-distal side. Guide RNA modifications allow modulation of the dCas R-loop stability.

    • Porter M. Hall
    • James T. Inman
    • Michelle D. Wang
    Article Open Access
  • This work provides molecular insights into the export of cyclic glucans by a bacterial ABC transporter. The findings expand our understanding of polysaccharide trafficking across bacterial membranes, as well as protein-sugar interactions in general.

    • Jaroslaw Sedzicki
    • Dongchun Ni
    • Christoph Dehio
    Article
  • The voltage-gated sodium channel NaV1.7 plays essential roles in pain sensation. The authors report cryo-EM structures of NaV1.7 in complexes with three pore blockers, elucidating distinct mechanisms of action of their modulation on NaV1.7.

    • Jiangtao Zhang
    • Yiqiang Shi
    • Daohua Jiang
    Article
  • Cryo-EM has facilitated structural studies of membrane proteins, but inactive GPCRs have remained inaccessible due to their small size. Robertson et al. demonstrate a common nanobody-based approach to streamline the determination of such structures.

    • Michael J. Robertson
    • Makaía M. Papasergi-Scott
    • Georgios Skiniotis
    Article