Reviews & Analysis

This PrimeView highlights the use of different twin study designs in behavioural genetics research to probe causal pathways.

Behavioural genetics examines the underlying factors associated with individual differences in behaviours and capacities. In this Primer, Willoughby et al. discuss the methods used in behavioural genetics analysis, in particular twin studies, genome-wide association studies and statistical modelling.

This PrimeView highlights the techniques used for single-cell pathogen diagnostics to accelerate treatment and contribute to responsible antimicrobial use.

Single-cell pathogen diagnostic tools are increasingly being used to identify and monitor bacterial infections and antimicrobial resistance. In this Primer, Li et al. discuss the assessment, design and implementation of single-cell pathogen diagnostics.

This PrimeView highlights the analytical steps involved in molecular QTL mapping to identify associations between variants and molecular phenotypes.

Molecular quantitative trait locus (molQTL) mapping associates genetic variation with molecular traits that can be measured as gene expression, splicing and chromatin accessibility. In this Primer, Aguet et al. discuss the study design and implementation of molQTL mapping in various applications, with a focus on technical developments for functional characterization.

This PrimeView highlights how the diverse properties of covalent organic frameworks can be used for a range of applications

Covalent organic frameworks (COFs) are a class of crystalline porous polymers consisting of highly ordered organic structures formed by polymerization. In this Primer, Tan et al. discuss the design principle, experimental methods, characterization and applications of COFs.

This PrimeView highlights how the PCA workflow reduces the dimensions of the original dataset to produce a biplot.

Principal component analysis is a multivariate statistical method that reduces a large number of variables into fewer variables, called principal components. This Primer describes how the method can be used for data analysis, explaining the mathematical background, analytical workflows, how to interpret a biplot and variants of the method.

This PrimeView highlights the trade-off between biological complexity and tunability when designing and fabricating hydrogels for use in mechanobiology.

Hydrogels are used to mimic cells’ local environment, enabling the study of cellular responses to biochemical and mechanical cues. Here, Blache et al. discuss the challenges of creating hydrogels for mechanobiology studies and how they can be used to analyse cell behaviour in the context of mechanobiological processes and harnessed to create regenerative therapies.

This PrimeView highlights key applications of tandem mass spectrometry in clinical laboratories.

Tandem mass spectrometry is increasingly utilized for clinical applications in laboratory medicine. In this Primer, Thomas et al. discuss experimental considerations and quality management for implementing clinical tandem mass spectrometry in the clinic with an overview of some key applications.

This PrimeView highlights the procedure for growing and maintaining organoids from human stem cells, with a focus on recent developments that have reduced a number of limitations in organoid development and research.

Organoids are cell-based in vitro models derived from stem cells, reconstituting the complex structure and function of the corresponding tissue. In this Primer, Zhao, Chen, Dowbaj, Sljukic, Bratlie, Lin et al. discuss the development of organoids and methods for controlling their cellular environment.

This PrimeView highlights different transduction mechanisms, protein mediators and magnetic nanotransducers that can be used to control cell signalling.

Magnetic nanomaterials can be used to transduce magnetic fields into biologically relevant signals. This Primer describes different magnetic transduction mechanisms, the design of nanotransducers and example applications for studying cell signalling and neuroscience.

This PrimeView highlights the use of intravital microscopy techniques to study a range of biological processes, including neurobiology, stem cell biology and cancer research.

Intravital microscopy (IVM) techniques are used to visualize intact and live tissues at the cellular and subcellular level. In this Primer, Scheele, Herrmann et al. discuss IVM in rodents, outlining challenges and opportunities for using the technique.