Reviews & Analysis

Filter By:

Article Type
  • Magnetic nanomaterials can be used to transduce magnetic fields into biologically relevant signals. This Primer describes different magnetic transduction mechanisms, the design of nanotransducers and example applications for studying cell signalling and neuroscience.

    • Gabriela Romero
    • Jimin Park
    • Polina Anikeeva
  • This PrimeView highlights the use of intravital microscopy techniques to study a range of biological processes, including neurobiology, stem cell biology and cancer research.

  • Intravital microscopy (IVM) techniques are used to visualize intact and live tissues at the cellular and subcellular level. In this Primer, Scheele, Herrmann et al. discuss IVM in rodents, outlining challenges and opportunities for using the technique.

    • Colinda L. G. J. Scheele
    • David Herrmann
    • Jacco van Rheenen
  • This PrimeView highlights the general experimental design and setup of imaging flow cytometry.

  • Imaging flow cytometry combines the high-event-rate nature of flow cytometry with single-cell image acquisition associated with microscopy. In this Primer, Rees et al. discuss the typical imaging flow instrumentation, the type of data acquired and how to analyse them.

    • Paul Rees
    • Huw D. Summers
    • Minh Doan
  • This PrimeView highlights how hydrogen produced from water electrolysis can be utilized for fuel and chemical feedstock.

  • Electrochemical water splitting using renewable electricity is a promising method for the sustainable production of hydrogen. This Primer overviews considerations, techniques and methods for water electrolysis and describes methods to improve rigour and reproducibility when analysing electrochemical data.

    • Arthur J. Shih
    • Mariana C. O. Monteiro
    • Marc T. M. Koper
  • Holographic microscopy is based on collecting holograms, patterns of dark and light fringes, and analysing them to obtain information about the specimen’s properties. This Primer introduces in-line holographic microscopy, with a focus on three analysis methods: generative modelling, machine learning and hybrid approaches.

    • Caroline Martin
    • Lauren E. Altman
    • Vinothan N. Manoharan
  • This PrimeView highlights the instrumentation for optical coherence tomography (OCT) in clinical settings, with an emphasis on Fourier domain OCT, spectral domain OCT and circular OCT.

  • Optical coherence tomography can be used as a conventional microscope, ophthalmic scanner or endoscope. In this Primer, Bouma et al. outline the instrumentation and data processing in obtaining topological and internal microstructure information from samples in three dimensions.

    • Brett E. Bouma
    • Johannes F. de Boer
    • Maciej Wojtkowski
  • The combination of single-cell RNA sequencing with metabolic RNA labelling enables a time-resolved view of transcriptional responses in individual cells. In this Primer, Erhard and Saliba et al. discuss metabolic labelling approaches and how to assess the temporal dynamics of transcriptional responses in different conditions.

    • Florian Erhard
    • Antoine-Emmanuel Saliba
    • Lars Dölken
  • This PrimeView highlights different complexity models that can be used for ocean biogeochemical modelling

  • Ocean biogeochemical models use coupled differential equations to describe the transformation of the ocean’s circulation, physical and biogeochemical properties under different conditions. This Primer introduces the process of model construction and explains the characteristics of various model types, from simple to complex, alongside their advantages and disadvantages.

    • Katja Fennel
    • Jann Paul Mattern
    • Liuqian Yu
  • This PrimeView highlights approaches to improve the reproducibility of in-depth interviews in the social sciences.

  • In-depth interviews are a versatile form of qualitative data collection used by researchers across the social sciences. In this Primer, Knott et al. describe the stages and challenges involved in designing and conducting interviews, how to analyse the data and strategies to overcome challenges.

    • Eleanor Knott
    • Aliya Hamid Rao
    • Chana Teeger
  • Electrochemical methods of CO2 separation offer potentially cheap, low-energy, scalable carbon capture technologies. In this Primer, Diederichsen et al. provide an overview of the experimentation and analysis needed for the study of electrochemical methods for CO2 separation.

    • Kyle M. Diederichsen
    • Rezvan Sharifian
    • T. Alan Hatton