DNA Origami

DNA origami involves the nanoscale folding of DNA to create pre-designed 2D and 3D shapes and patterns.

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  • Ule and colleagues discuss cross-linking and immunoprecipitation (CLIP) methods for characterizing the RNA binding partners of RNA-binding proteins and explore the data analysis workflows, best practices and applications for these techniques. The Primer also considers methods for characterizing the protein binding partners of specific RNAs and discusses how data from these complementary methods can be integrated into CLIP workflows.

    • Markus Hafner
    • Maria Katsantoni
    • Mihaela Zavolan
    Primer
  • This Primer on X-ray computed tomography explores the different experimental configurations for three-dimensional data acquisition as well as the fundamentals of three-dimensional data reconstruction, segmentation and analysis with examples across the physical and life sciences.

    • Philip J. Withers
    • Charles Bouman
    • Stuart R. Stock
    Primer
  • This Primer outlines the best ways to find, handle and analyse ancient DNA from various sources. The authors summarize ethical considerations and the importance of working closely with all stakeholders, including archaeologists, curators and descendant communities.

    • Ludovic Orlando
    • Robin Allaby
    • Christina Warinner
    Primer
  • Dey et al. discuss the design and implementation of DNA origami, as well as the techniques used to analyse quality and construction. They summarize exciting new research areas where DNA nanotechnology is being used and future directions for the field.

    • Swarup Dey
    • Chunhai Fan
    • Pengfei Zhan
    Primer
  • This Primer on chromatin accessibility profiling methods discusses differences in the methods commonly used to determine chromatin states in different cell types, including ATAC-seq and ChIP–seq. The authors summarize applications in different areas of research, from single cells to tissues and whole organisms.

    • Liesbeth Minnoye
    • Georgi K. Marinov
    • Stein Aerts
    Primer
  • This Primer on chemical vapour deposition summarizes current and emerging experimental set-ups as well as common characterization approaches used to determine thin film formation and quality as applied to graphene and other novel 2D materials.

    • Luzhao Sun
    • Guowen Yuan
    • Zhongfan Liu
    Primer
  • This PrimeView highlights important experimental and analytical aspects of conducting ancient DNA analysis, with a focus on collaboration and sample preservation.

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  • This PrimeView highlights the design, assembly and assessment of 2D and 3D nanostructures made using DNA origami.

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  • This PrimeView highlights the main chromatin accessibility profiling methods. These methods include DNase-seq, ATAC-seq, MNase-seq, and DNA methylation-based methods to assess open chromatin and regulatory elements.

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  • This PrimeView highlights the process of producing 2D materials using chemical vapour deposition. Advances in this technique have increased yield and quality, and are being used for industrial applications.

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  • Bayesian statistics are an approach to data analysis based on Bayes’ Theorem. This PrimeView provides an overview of how to select and establish priors, likelihoods, and posteriors. The use of Bayesian statistics and modelling across disciplines is discussed, along with appropriate sharing of data acquired using Bayesian methods.

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  • This PrimeView highlights recombination-mediated genome engineering methods common in microbial synthetic biology. The use of one technique in particular, MAGE, is discussed in terms of its optimization and use in biotechnology and to improve human health.

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