Volume 18 Issue 5, May 2017

Volume 18 Issue 5

'Shedding light on phase separation' by Vicky Summersby, inspired by the Review on p285.

Research Highlights

Progress

  • Progress |

    DNA G-quadruplexes (G4s) are guanine-rich sequences that fold into four-stranded structures. Recent progress in the detection and mapping of genomic G4 structures has provided new insights into their functions in regulating transcription and genome stability, and has revealed their potential relevance for cancer therapy.

    • Robert Hänsel-Hertsch
    • , Marco Di Antonio
    •  & Shankar Balasubramanian

Reviews

  • Review Article |

    In addition to membrane-bound organelles, eukaryotic cells feature various membraneless compartments, including the centrosome, the nucleolus and various granules. Many of these compartments form through liquid–liquid phase separation, and the principles, mechanisms and regulation of their assembly as well as their cellular functions are now beginning to emerge.

    • Salman F. Banani
    • , Hyun O. Lee
    • , Anthony A. Hyman
    •  & Michael K. Rosen
  • Review Article |

    Core histone proteins are deposited on chromatin during DNA replication, whereas their replication-independent variants are deposited throughout the cell cycle by specific chaperones and chromatin remodellers. This dynamic deposition of histone variants has important roles in cell fate specification and has been implicated in development and tumorigenesis.

    • Marcus Buschbeck
    •  & Sandra B. Hake
  • Review Article |

    Structure-specific endonucleases (SSEs) function in concert with other DNA-remodelling enzymes and cell cycle control machineries in processes such as DNA adduct repair, Holliday junction processing and the response to replication stress. As SSEs have specificity for DNA structures rather than sequence, tight regulation of their activity is important to ensure genome stability.

    • Pierre-Marie Dehé
    •  & Pierre-Henri L. Gaillard

    Series:

Perspectives

    Opinion

  • Opinion |

    Although non-coding RNAs have roles in transcription and chromatin function, nascent pre-mRNA is usually considered to be passive during these processes. Recently identified interactions between nascent pre-mRNAs and regulatory proteins suggest that both types of RNA regulate transcription and chromatin function.

    • Lenka Skalska
    • , Manuel Beltran-Nebot
    • , Jernej Ule
    •  & Richard G. Jenner