Volume 17 Issue 12, December 2016

Volume 17 Issue 12

'The 3D world of the nucleus' by Vicky Summersby, inspired by this Focus issue.

Research Highlights

Comment

  • Comment |

    Job Dekker asserts that cases in which data from microscopy- and 3C-based methods appear discordant about genome organization will provide opportunities to improve our models of chromatin folding.

    • Job Dekker

    Collection:

Reviews

  • Review Article |

    The three-dimensional (3D) organization of eukaryote chromosomes regulates genome function and nuclear processes such as DNA replication, transcription and DNA-damage repair. Experimental and computational methodologies for 3D genome analysis have been rapidly expanding, with a focus on high-throughput chromatin conformation capture techniques and on data analysis.

    • Anthony D. Schmitt
    • , Ming Hu
    •  & Bing Ren
  • Review Article |

    Mechanistic insights are emerging into how long non-coding RNAs (lncRNAs) regulate gene expression by coordinating regulatory proteins, localizing to genomic loci and shaping nuclear organization. Interestingly, lncRNAs can perform functions that cannot be carried out by DNA elements or proteins alone, such as amplifying regulatory signals in the nucleus.

    • Jesse M. Engreitz
    • , Noah Ollikainen
    •  & Mitchell Guttman

    Collection:

  • Review Article |

    Mutations in non-coding parts of the genome can cause disease. Technological advances are providing unprecedented detail on genome organization and folding, and have revealed that enhancer–target gene coupling is spatially restricted, as it occurs within topologically associated domains (TADs), and that disrupting such organization can lead to disease-associated gene dysregulation.

    • Peter Hugo Lodewijk Krijger
    •  & Wouter de Laat

    Collection:

  • Review Article |

    Steroid hormone receptors are well known to regulate various aspects of animal physiology by acting as transcriptional regulators in the nucleus. However, it is now evident that these receptors can also be targeted to extranuclear locations (such as the plasma membrane), where they instigate rapid signals that contribute to steroid-mediated cellular responses.

    • Ellis R. Levin
    •  & Stephen R. Hammes

Perspectives

    Opinion

  • Opinion |

    The establishment of various coexisting actin networks supports a plethora of cellular processes and functions. How actin incorporation into these different networks is regulated to balance their growth and maintain homeostasis has remained elusive. Here, the authors propose that the internetwork competition for a limited pool of actin monomers underlies the homeostatic control of actin cytoskeleton organization.

    • Cristian Suarez
    •  & David R. Kovar