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In this Tools of the Trade article, Bohan Zhang (Gladyshev lab) discusses how the use of extended heterochronic parabiosis in mice (surgical connection between circulatory systems for an extended period), followed by detachment, can shed light on potential mechanisms that reverse mammalian ageing.
Phosphorylation of the mitochondrial DNA (mtDNA) maintenance factor TFAM during spermatogenesis prevents its import into mitochondria, resulting in elimination of paternal mtDNA and leading to maternal inheritance.
Ernst and Renne highlight two papers, one that discovered and another that structurally defined the ER–mitochondria encounter structure (ERMES) that facilitates the exchange of lipids between the ER and mitochondria.
In this Tools of the Trade article, Christine Lim and Alicia González Díaz (Vendruscolo lab) describe a pipeline for the identification and characterization of proteins whose phase separation is altered in disease.
mTOR inhibition by deprivation of the amino acids Tyr, Trp and Phe promotes the translocation of the 26S proteasome from the nucleus to the cytosol, which is required for stress tolerance and cell survival.
Sarkar et al. identify a feedback loop between the methyltransferase SETDB1 and nuclear pore complexes that ensures silencing of early-oogenesis genes as oocyte specification progresses.
In this Tools of the Trade article, Sarah Paramore (from the Devenport and Nelson labs) discusses the use of mouse strains carrying genomic alterations in PCP genes and how they can increase our understanding of mammalian planar cell polarity.
Antennapedia proteins were among the first proteins found to be exchanged intercellularly. This discovery by Alain Prochiantz and colleagues has inspired researchers of various backgrounds.
Liang et al. show that mitochondria can be expelled from cells via extracellular vesicles as a route of quality control that is an alternative to lysosomal degradation.
Bulut-Karslıoğlu remembers the publication of two seminal papers that described bivalent chromatin and how this discovery continues to affect research to this day.
To activate noncanonical LC3B lipidation and NLRP3 inflammasome formation, stimulator of interferon genes (STING) forms a proton channel in Golgi membranes.
Anne West recounts the study that showed postnatal accumulation of non-CpG DNA methylation in neurons coinciding with postnatal synapse maturation, suggesting that it contributes to brain function.
In the amphibian axolotl, the kinase mTOR is hyper-sensitive and activates a protein synthesis response that is crucial for wound healing and tissue regeneration.
In this Tools of the Trade article, Joleen Cheah (from the Ting lab) discusses the development of TransitID, which allows researchers to capture protein trafficking pathways in vivo without a priori knowledge of specific proteins of interest.
Cremer et al. show that the ubiquitin ligase RNF26 and vimentin-based intermediate filaments cooperate to control perinuclear ER membrane organization and thus facilitate recovery from ER stress.