News & Comment

In this Tools of the Trade article, Jeroen van den Berg (van Oudenaarden lab) presents a new method based on pulse-labelling of nascent DNA to study the dynamics of DNA replication in single cells.

This study finds that microtubules act as a mechanostat during cell migration, becoming mechanically reinforced in response to compression to protect the nucleus and coordinate contractility.

Stresses induce de-crowding and fluidization of the cytoplasm, which promotes the formation of biomolecular condensates.

MYB-related transcription factors are found to function in chloroplast biogenesis alongside GLK in the distantly related species Marchantia polymorpha and Arabidopsis thaliana.

Abigail Buchwalter recounts what happened to the nuclei of cells lacking all lamin genes.

Paul Nurse discusses how a 1971 paper by Culotti and Hartwell inspired him to investigate the cell cycle in fission yeast, and how these genetics studies led to the discovery of cyclin-dependent kinases.

The studies that paved the way for the development of PROTACs (proteolysis-targeting chimeras) as therapeutic strategies, and the HPV vaccine.

Many proteins in the mouse ovary are extremely stable; they enhance proteostasis and limit protein aggregation, thereby supporting the maintenance of the long-lived oocytes.

An elegant study revealed the distinct roles of different H3K9 methylation states in heterochromatin formation and function.

In a recent study, Bong et al. identify a polarized distribution of contact sites between the endoplasmic reticulum and plasma membrane in migrating cells, whereby higher density of contacts in the back of the cells prevents the formation of additional migration fronts.

This Comment discusses erroneous reporting of mass spectrometry analyses of lipids in mammalian samples, and provides recommendations for how to avoid it.

This Comment discusses the progress, limitations and best practices in applying foundation models to single-cell omics data.

In this Tools of the Trade article, Reiniš and Reicher (Kubicek Lab) describe the development of a multicolour protein-tagging strategy that allows live-cell monitoring of multiple proteins.

This study uncovered a link between PI5P, PI5P4Ks and the Hippo pathway, whereby PI5P reinforces the inhibition of YAP, the effector of the Hippo pathway.

Kawaguchi et al. identify a chromatin code based on repressive histone 3 lysine 27 trimethylation (H3K27me3) that serves as a segment-specific positional marker in connective tissue cells of axolotls, enabling correct patterning during limb regeneration.

Verheyen and Gottardi revisit two seminal papers by the Basler, Peifer and Clevers labs elucidating the role of nuclear β-catenin in Wnt signal transduction through its interaction with TCF at Wnt target genes.

Cleavage of promoter-upstream antisense RNAs by the Integrator complex supports the preferential transcription of pre-mRNAs.

Claire Durrant reminds us of the importance of studying the physiological roles of proteins and their aggregates to understand their roles in disease and inform therapies, discussing a 2008 paper on amyloid-β from the Arancio lab.

Stephanie Moon discusses findings that revealed that ribosome stalling can lead to the suppression of translation initiation in the brain, delaying the onset of neurodegeneration.

Robert Parton and colleagues discuss novel evidence on the role of dynamin in caveolar endocytosis, which calls into question established models of dynamin-mediated fission.