Biomolecular condensates are emerging as hubs of splicing regulation. This Review discusses the modulation of condensate functions through alternative splicing, regulation of (co-transcriptional) splicing at condensates and the involvement of these condensates in human diseases.

Ferroptosis is a non-apoptotic, iron-dependent cell death mechanism driven by plasma membrane lipid peroxidation and subsequent plasma membrane rupture. Various cellular compartments and organelles contribute to regulating susceptibility to ferroptosis. This regulation involves a plethora of mechanisms centred on iron metabolism and storage, lipid metabolism, and redox balance.

De Caestecker and Macara find that the sorting of membrane proteins in the Golgi relies on a size filter that enables correct localization of proteins with a short cytosolic domain to the apical membrane.

Elias Barriga discusses a seminal 2006 paper from Zhao et al., which was the first study to integrate electrotaxis signals into the established molecular framework enabling directed cell migration.

mRNAs with alternative in-frame translation initiation sites can yield proteoforms with distinct localization and functions.

In this Tools of the Trade article, Jeroen van den Berg (van Oudenaarden lab) presents a new method based on pulse-labelling of nascent DNA to study the dynamics of DNA replication in single cells.

This study finds that microtubules act as a mechanostat during cell migration, becoming mechanically reinforced in response to compression to protect the nucleus and coordinate contractility.

Stresses induce de-crowding and fluidization of the cytoplasm, which promotes the formation of biomolecular condensates.