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  • Filtering genomic data is a crucial step to ensure the quality and reliability of downstream analyses. The authors provide guidance on the choice of filtering strategies and thresholds, including filters that remove sequencing bases or reads, variants, loci, genotypes or individuals from genomic datasets to improve accuracy and reproducibility.

    • William Hemstrom
    • Jared A. Grummer
    • Mark R. Christie
    Review Article
  • In this Journal Club article, Laura Ross discusses several seminal papers that describe the discovery of germline-specific chromosomes and paternal genome elimination, striking examples of non-Mendelian genetics.

    • Laura Ross
    Journal Club
  • In this Tools of the Trade article, Francisco Lorenzo-Martín and Matthias Lutolf present mini-colons as a new ex vivo cancer model that incorporates microfabrication, tissue engineering and optogenetics.

    • L. Francisco Lorenzo-Martín
    • Matthias P. Lutolf
    Tools of the Trade
  • In this Tools of the Trade article, Saori Sakaue describes SCENT, a tool to generate cell-type-specific enhancer–gene maps using single-cell multi-omics data, which can help identify disease-causal, non-coding variants and genes from GWAS-defined loci.

    • Saori Sakaue
    Tools of the Trade
  • Despite their impact on human complex traits and diseases, gene–environment interactions (G × E) remain challenging to assess statistically. The authors review considerations for the conceptualization, methodology, interpretation and reporting of G × E studies, and provide recommendations on how to avoid common pitfalls.

    • Esther Herrera-Luis
    • Kelly Benke
    • Genevieve L. Wojcik
    Review Article
  • In this Journal Club, Jessica Tollkuhn discusses how a paper describing genome-wide application of chromatin immunoprecipitation (ChIP)-on-chip inspired her own research into oestrogen-based gene regulation in the brain.

    • Jessica Tollkuhn
    Journal Club
  • Mustafa Mir reflects on a 1976 paper by McKnight and Miller, in which they developed a technique to directly visualize gene regulatory dynamics.

    • Mustafa Mir
    Journal Club
  • In this Review, Stanley summarizes the role of genetics in mammalian glycosylation, highlighting how advances in genetic and genomic technologies are helping to characterize the genes involved and contributing to the development of therapies for diseases related to glycosylation.

    • Pamela Stanley
    Review Article
  • Logsdon et al. report the second complete sequence of all centromeres from a single human genome, enabling comparative analyses of the variation in tandemly repeating α-satellite DNA.

    • Kirsty Minton
    Research Highlight
  • In this Comment, Lamkin and Gymrek discuss recent results that suggest that the systematic incorporation of tandem repeats into complex trait analyses will yield a rich source of causal variants and new biological insights.

    • Michael Lamkin
    • Melissa Gymrek
  • In this Tools of the Trade article, Samuel Gould explains how prime editing sensors can improve experimental efficiency and can be designed using a computational tool he created and named PEGG.

    • Samuel I. Gould
    Tools of the Trade
  • Renée Beekman discusses the possibilities for research into transient enhancers by highlighting a recent paper by Vermunt et al. that identifies how they can modulate gene silencing dynamics.

    • Renée Beekman
    Journal Club
  • In this Journal Club, Kirstyn Brunker highlights two papers published in 2017 that showcase how the emergence of portable sequencing capabilities improved the real-time response to infectious disease outbreaks on a global scale.

    • Kirstyn Brunker
    Journal Club
  • In this Journal Club article, Olivia Rissland describes how a 1987 paper by Don Cleveland and colleagues provided insight into co-translational gene regulation of tubulin.

    • Olivia S. Rissland
    Journal Club
  • Targeted genome modification using CRISPR–Cas genome editing, base editing or prime editing is driving base research in plants and precise molecular breeding. The authors review the technological principles underlying these methods, approaches for their delivery in plants, and emerging crop-breeding strategies based on targeted genome modification.

    • Boshu Li
    • Chao Sun
    • Caixia Gao
    Review Article
  • In this Review, Li and Durbin discuss how to generate telomere-to-telomere assemblies for large haploid or diploid genomes using currently available data types and algorithms, and outline remaining challenges in resolving highly repetitive sequences and polyploid genomes.

    • Heng Li
    • Richard Durbin
    Review Article
  • In this Review, the authors summarize our current understanding of nuclear pre-mRNA and mRNA decay pathways. They describe how aberrantly processed mRNAs are targeted for decay in the nucleus and how this process is regulated to finely control gene expression.

    • Xavier Rambout
    • Lynne E. Maquat
    Review Article
  • In this Perspective, Werner and colleagues discuss the many potential mechanisms by which natural antisense transcripts (NATs) can regulate expression of their complementary sense transcripts, the biological implications of their regulatory effects and the potential of NATs for therapeutic applications.

    • Andreas Werner
    • Aditi Kanhere
    • John S. Mattick