In this Tools of the Trade article, Francisco Barriga describes the development and use of MACHETE, a flexible deletion engineering strategy, which enables the functional characterization of the large genomic deletions that occur in cancer.

In this study, the authors investigate the mechanistic basis of alternative telomere lengthening, a process that certain cancer cells use to overcome telomere shortening.

Altea-Manzano et al. find that factors secreted by primary breast tumours or high-fat diet feeding enriches the fatty acid palmitate in distant organs, which primes pre-metastatic niches enabling metastatic growth.

In this study, Linde et al. demonstrate how therapeutic activation of neutrophils leads to tumour eradication and reduced metastatic seeding in multiple mouse models of cancer.

In this Tools of the Trade article, Susan Bullman describes the development and use of INVADEseq, a single-cell RNA sequencing approach that facilitates the simultaneous capture of both eukaryotic and bacterial transcripts in host cells to functionally understand the intratumoural microbiota.

In this Tools of the Trade article, Lee describes Select-seq, a novel method for generating epitranscriptomic data from microniches within the tumour. The author reveals how the method can be used to identify novel biomarkers from rare cell subpopulations such as cancer stem cells in tumours.