Volume 8 Issue 7, July 2013

Efficient adipocyte differentiation (>90%) of multilineage-differentiating, stress-enduring (Muse) cells. The presence of cells containing lipid droplets positive for oil red staining indicates that adipocyte differentiation has occurred. Image taken from the protocol by Kuroda et al. Cover design by Jamel Wooten.

Protocol

Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy

Ionel Popa
Pallav Kosuri
Julio M Fernandez
Protocol 06 Jun 2013
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Small-molecule ligand docking into comparative models with Rosetta

Steven A Combs
Samuel L DeLuca
Jens Meiler
Protocol 06 Jun 2013
Sub-millisecond ligand probing of cell receptors with multiple solution exchange

Sergiy Sylantyev
Dmitri A Rusakov
Protocol 06 Jun 2013
Time-resolved fluorescence ligand binding for G protein–coupled receptors

Alexander Emami-Nemini
Thomas Roux
Martin J Lohse
Protocol 13 Jun 2013
Ligand-based receptor identification on living cells and tissues using TRICEPS

Andreas P Frei
Hansjoerg Moest
Bernd Wollscheid
Protocol 13 Jun 2013
Genome-wide measurement of protein-DNA binding dynamics using competition ChIP

Colin R Lickwar
Florian Mueller
Jason D Lieb
Protocol 13 Jun 2013
Isolation, culture and genetic manipulation of mouse pancreatic ductal cells

Maximilian Reichert
Shigetsugu Takano
Anil K Rustgi
Protocol 20 Jun 2013
Studying synapses in human brain with array tomography and electron microscopy

Kevin R Kay
Colin Smith
Tara L Spires-Jones
Protocol 20 Jun 2013
A bacterial export system for generating extracellular amyloid aggregates

Viknesh Sivanathan
Ann Hochschild
Protocol 20 Jun 2013
Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells

Yasumasa Kuroda
Shohei Wakao
Mari Dezawa
Protocol 20 Jun 2013
Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts

Francois-Xavier Theillet
Honor May Rose
Philipp Selenko
Protocol 27 Jun 2013
Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization

Tobias Starborg
Nicholas S Kalson
Karl E Kadler
Protocol 27 Jun 2013
Selective monitoring of ubiquitin signals with genetically encoded ubiquitin chain–specific sensors

Sjoerd J L van Wijk
Evgenij Fiškin
Ivan Dikic
Protocol 27 Jun 2013

Volume 8 Issue 7, July 2013

Protocol

Force dependency of biochemical reactions measured by single-molecule force-clamp spectroscopy

Small-molecule ligand docking into comparative models with Rosetta

Sub-millisecond ligand probing of cell receptors with multiple solution exchange

Time-resolved fluorescence ligand binding for G protein–coupled receptors

Ligand-based receptor identification on living cells and tissues using TRICEPS

Genome-wide measurement of protein-DNA binding dynamics using competition ChIP

Isolation, culture and genetic manipulation of mouse pancreatic ductal cells

Studying synapses in human brain with array tomography and electron microscopy

A bacterial export system for generating extracellular amyloid aggregates

Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells

Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts

Using transmission electron microscopy and 3View to determine collagen fibril size and three-dimensional organization

Selective monitoring of ubiquitin signals with genetically encoded ubiquitin chain–specific sensors

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