Volume 8 Issue 6, June 2013

Droplet interface bilayers are created by contacting nanoliter aqueous droplets in an oil solution in the presence of phospholipids. This image was created by manipulating a white-light microscopy image of a bilayer formed between an aqueous droplet and an agarose hydrogel. Taken from the protocol by Leptihn et al. Cover design by Jamel Wooten.

Protocol

Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast

Daphne H E W Huberts
Sung Sik Lee
Matthias Heinemann
Protocol 02 May 2013
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Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells

Kathy K Niakan
Nadine Schrode
Anna-Katerina Hadjantonakis
Protocol 02 May 2013
Preparation and purification of zinc sulfinate reagents for drug discovery

Fionn O'Hara
Ryan D Baxter
Phil S Baran
Protocol 02 May 2013
Constructing droplet interface bilayers from the contact of aqueous droplets in oil

Sebastian Leptihn
Oliver K Castell
Mark I Wallace
Protocol 02 May 2013
Measurement of phosphoinositides in the zebrafish Danio rerio

David R Jones
Irene Barinaga-Rementeria Ramirez
Nullin Divecha
Protocol 09 May 2013
Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood

Giulietta Saletti
Nicolas Çuburu
Cecil Czerkinsky
Protocol 09 May 2013
FACS purification of Drosophila larval neuroblasts for next-generation sequencing

Heike Harzer
Christian Berger
Juergen A Knoblich
Protocol 09 May 2013
Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization

Samuel O Skinner
Leonardo A Sepúlveda
Ido Golding
Protocol 16 May 2013
Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish

Kevin Takaki
J Muse Davis
Lalita Ramakrishnan
Protocol 16 May 2013
Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies

Conor Fields
David O'Connell
Julien Muzard
Protocol 16 May 2013
Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease

Annika Mehlem
Carolina E Hagberg
Annelie Falkevall
Protocol 23 May 2013
Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS

Nan Li
Chi-Lin Kuo
Bogdan I Florea
Protocol 23 May 2013
High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis

Boštjan Rituper
Alenka Guček
Robert Zorec
Protocol 23 May 2013
Two-way communication with neural networks in vivo using focused light

Nathan R Wilson
James Schummers
Mriganka Sur
Protocol 23 May 2013
Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro

Li Qian
Emily C Berry
Deepak Srivastava
Protocol 30 May 2013
Femtosecond optical transfection of individual mammalian cells

Maciej Antkowiak
Maria L Torres-Mapa
Frank J Gunn-Moore
Protocol 30 May 2013
Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing

Rachel Yuan Nong
Di Wu
Spyros Darmanis
Protocol 30 May 2013
Preparation and use of MitoPY1 for imaging hydrogen peroxide in mitochondria of live cells

Bryan C Dickinson
Vivian S Lin
Christopher J Chang
Protocol 30 May 2013

Volume 8 Issue 6, June 2013

Protocol

Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast

Derivation of extraembryonic endoderm stem (XEN) cells from mouse embryos and embryonic stem cells

Preparation and purification of zinc sulfinate reagents for drug discovery

Constructing droplet interface bilayers from the contact of aqueous droplets in oil

Measurement of phosphoinositides in the zebrafish Danio rerio

Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood

FACS purification of Drosophila larval neuroblasts for next-generation sequencing

Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization

Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish

Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies

Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease

Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS

High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis

Two-way communication with neural networks in vivo using focused light

Reprogramming of mouse fibroblasts into cardiomyocyte-like cells in vitro

Femtosecond optical transfection of individual mammalian cells

Solid-phase proximity ligation assays for individual or parallel protein analyses with readout via real-time PCR or sequencing

Preparation and use of MitoPY1 for imaging hydrogen peroxide in mitochondria of live cells

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