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Mass spectrometry of membrane protein complexes reveals phospholipid binding and subunit stoichiometry. Shown are mass spectra of the pentameric ligand-gated ion channel from Erwinia chrysanthemi titrated with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE). Up to seven lipid-bound states can be resolved within the stripped tetramer. Image taken from the protocol by Laganowsky et al. Cover design by Jamel Wooten.