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Volume 6 Issue 8, August 2011

Astrocytes generated at physiological oxygen tension from human embryonic stem cell–derived neural precursors. Immature astrocytes express vimentin (green), whereas a more mature subpopulation also expresses glial fibrillary acidic protein (GFAP, red). Cell nuclei are stained with DAPI (blue). The protocol describes efficient neural precursor cell, motor neuron and dopaminergic neuron generation at 3% oxygen.



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