Volume 6 Issue 11, November 2011

Human astrocytes with functional properties and regional specificity are generated by the protocol described in this issue through the differentiation of pluripotent stem cells to the gliogenic stage. These astrocytes have a star-like appearance, express the glial progenitor marker S100β (red), and at mature stages form filaments expressing GFAP (green). Image taken from the protocol by Krencik & Zhang.

Protocol

Using the T-Coffee package to build multiple sequence alignments of protein, RNA, DNA sequences and 3D structures

Jean-Francois Taly
Cedrik Magis
Cedric Notredame
Protocol 06 Oct 2011
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Selective 2′-hydroxyl acylation analyzed by protection from exoribonuclease (RNase-detected SHAPE) for direct analysis of covalent adducts and of nucleotide flexibility in RNA

Kady-Ann Steen
Nathan A Siegfried
Kevin M Weeks
Protocol 06 Oct 2011
Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry

Rita Casadonte
Richard M Caprioli
Protocol 13 Oct 2011
Directed differentiation of functional astroglial subtypes from human pluripotent stem cells

Robert Krencik
Su-Chun Zhang
Protocol 13 Oct 2011
Preparation of ¹⁸F-labeled peptides using the copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition

Herman S Gill
Jan Marik
Protocol 13 Oct 2011
Generation and differentiation of microtissues from multipotent precursor cells for use in tissue engineering

Fabian Langenbach
Karin Berr
Jörg Handschel
Protocol 13 Oct 2011
Low-bias, strand-specific transcriptome Illumina sequencing by on-flowcell reverse transcription (FRT-seq)

Lira Mamanova
Daniel J Turner
Protocol 20 Oct 2011
Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

Ralf Schröder
Johannes Schmidt
Evi Kostenis
Protocol 20 Oct 2011
Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning

David C Appleyard
Stephen C Chapin
Patrick S Doyle
Protocol 20 Oct 2011
Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitors

Norbert Berndt
Saïd M Sebti
Protocol 27 Oct 2011
Ex vivo time-lapse confocal imaging of the mouse embryo aorta

Jean-Charles Boisset
Charlotte Andrieu-Soler
Catherine Robin
Protocol 27 Oct 2011
Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast

Clara Bermejo
Farzad Haerizadeh
Wolf B Frommer
Protocol 27 Oct 2011
Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells

Bi-Huei Hou
Hitomi Takanaga
Wolf B Frommer
Protocol 27 Oct 2011

Volume 6 Issue 11, November 2011

Protocol

Using the T-Coffee package to build multiple sequence alignments of protein, RNA, DNA sequences and 3D structures

Selective 2′-hydroxyl acylation analyzed by protection from exoribonuclease (RNase-detected SHAPE) for direct analysis of covalent adducts and of nucleotide flexibility in RNA

Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry

Directed differentiation of functional astroglial subtypes from human pluripotent stem cells

Preparation of ¹⁸F-labeled peptides using the copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition

Generation and differentiation of microtissues from multipotent precursor cells for use in tissue engineering

Low-bias, strand-specific transcriptome Illumina sequencing by on-flowcell reverse transcription (FRT-seq)

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

Bar-coded hydrogel microparticles for protein detection: synthesis, assay and scanning

Measurement of protein farnesylation and geranylgeranylation in vitro, in cultured cells and in biopsies, and the effects of prenyl transferase inhibitors

Ex vivo time-lapse confocal imaging of the mouse embryo aorta

Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast

Optical sensors for monitoring dynamic changes of intracellular metabolite levels in mammalian cells

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