Volume 18

  • No. 11 November 2023

    A proximal tubule-enhanced kidney organoid derived from induced pluripotent stem cells

    By more precisely recapitulating metanephric nephron progenitor specification, the authors generated induced pluripotent stem cell-derived kidney organoids with enhanced proximal tubule maturation and striking nephron spatial arrangement driven by a centralized source of WNT antagonism. See Vanslambrouck et al.

  • No. 10 October 2023

    On-chip electrocatalytic microdevices

    On-chip electrocatalytic microdevices allow high-precision electrocatalytic measurements at the individual nanomaterial level and offer unique perspectives that are not possible using conventional electrochemical methods. The cover shows an emerging electrochemical platform that specializes in investigating nanocatalysts/nanostructures at the microscopic level. See Wang et al.

  • No. 9 September 2023

    Human neural culture on a chip recapitulates neuron–glia interactions.

    The cover shows a 3D human neural culture on a chip, which allows the engagement of neurons with only disease-associated microglia, and their separation out from a heterogeneous microglia population. The image shows membrane-labeled microglia (red), GFP-labeled neuron and astrocyte (yellow), and immunostaining for astrocyte marker GFAP (blue). See Kang et al. p2838.

  • No. 8 August 2023

    Acoustic tweezers for single-cell analysis.

    Acoustic tweezers enable high-throughput, selective, precise and programmable manipulation of individual cells and particles, addressing interdisciplinary challenges in biology, materials science and soft-matter physics. See Yang et al.

  • No. 7 July 2023

    Hepatocyte generation in adult liver during tissue homeostasis

    Liver sections from hepatocyte-specific ProTracer mice are immunostained for tdTomato (green pseudo-color) and liver lobule zonation markers—E-cadherin (purple) and glutamine synthetase (red)—to reveal the highly regional proliferation of hepatocytes. See Liu et al.

  • No. 6 June 2023

    Scalable genome editing in zebrafish with MIC-Drop

    The cover shows a microinjection needle filled with hundreds of multiplexed intermixed CRISPR droplets (MIC-Drop). Each droplet contains the components needed to edit a different zebrafish gene, and one droplet is injected into each of the zebrafish embryos shown in the background. See Parvez et al.

  • No. 5 May 2023

    Human blastoids model blastocyst-stage embryos

    The cover shows a confocal image of a human blastoid labeled for the tight junction molecule ZO-1 (yellow), the adherens junction molecule CDH1 (magenta), the apical domain molecule aPKC (cyan) and nuclei (blue).

    See Heidari Khoei et al.

  • No. 4 April 2023

    Whole-mouse imaging at the cellular level

    Nanobody(VHH)-boosted 3D imaging of solvent-cleared organs (vDISCO) enables the labelling of single cells in intact animals or whole organs. The cover shows a vDISCO image of a whole mouse where individual metastatic cells (magenta), bones and organs (cyan) and muscles (yellow) are labelled via nanobodies.

    See Cai et al.

  • No. 3 March 2023

    Microcrystals of fatty acid photodecarboxylase

    Fatty acid photodecarboxylase is a photoenzyme with potential green chemistry applications. Structural insight into its enzymatic mechanism can be obtained by time-resolved crystallography. Efficient reaction initiation requires homogeneously sized microcrystals. Such crystals are shown here, they were grown using the seeding protocols described in this issue.

    See Shoeman et al.

  • No. 2 February 2023

    Intestinal organoid

    Mouse intestinal organoid immunostained for proliferating cells (Ki67 in yellow), cell adhesion (p120 in pink) and nuclei (Hoechst in blue).

    See Horvath et al.

  • No. 1 January 2023

    3D-printed optogenetic probes for wireless neuromodulation

    A 3D-printing-based manufacturing approach enables facile and rapid development of optogenetic neural probes of any desired design, length and dimension. These probes can be easily assembled with a tiny Bluetooth module to allow wireless optogenetics in freely behaving animals for complex behavioral neuroscience studies.

    See Parker et al.