Volume 15

  • No. 12 December 2020

    Glioblastoma organoid

    The cover shows a patient-derived glioblastoma organoid containing morphologically heterogeneous cell populations with radially oriented cells surrounding a necrotic center and more differentiated progenitor and glial cells toward the periphery. Image shows immunostaining with glioma stem cell marker NESTIN (cyan), glioma stem cell and astrocyte marker HOPX (magenta), and proliferation marker EdU (green).

    See Jacob et al.

  • No. 11 November 2020

    Oligodendrocyte precursor and mature cells in culture at the end of the oligodendrocyte induction protocol

    Immunofluorescence image of human pluripotent stem cell–derived cells expressing the oligodendrocyte precursor and mature cell marker O4 (red) at the end of the SOX10-mediated oligodendrocyte induction protocol. Cell nuclei are counterstained with Hoechst (blue).

    See García León et al.

  • No. 10 October 2020

    Probing the molecular context of proteins

    This cover illustrates the applicability of the MAC (Multiple Approaches Combined)-tag system to probe the interactome and molecular context of the protein of interest. In this issue, Liu et al. describe a streamlined AP-MS and BioID protocol and a data analysis web tool that, together, provide an easy to way to interpret the subcellular distribution of any protein of interest.

    See Liu et al.

  • No. 9 September 2020

    Phage drugs

    This cover reimagines the future of antibacterial ‘drug’ production as one driven by bacteriophages. Phages hijack bacterial machinery and self-amplify to create hundreds of new ‘phage drugs’. Phage therapy is being reintroduced to Western medicine, but researchers currently struggle to meet the clinical demands for quantity and quality. In this issue, Luong et al. present a systematic procedure for upscale production and purification suitable for clinical application.

    See Luong et al.

  • No. 8 August 2020

    Pupillometry as a readout of locus coeruleus activation

    Merged image of a human iris (white) and noradrenergic neurons (yellow) of the locus coeruleus from a DBH-iCre mouse. Visualization of the noradrenergic neurons is a consequence of triple-labeling for tyrosine hydroxylase (green), virally encoded floxed mCherry (red, delivered via an AAV-5 stereotactic injection) and a neuronal marker (Nissl bodies, blue).

    See Privitera et al.

  • No. 7 July 2020

    Scanning electron micrograph of a cell (pink) that is semi-encapsulated by DNA hydrogel (blue) (pseudocolored).

    MCF-7 cell captured as a consequence of an aptamer-trigger-clamped hybridization chain reaction (atcHCR). The 3D environment of the DNA networks minimizes cell damage, and the cells can subsequently be released for live-cell analysis.

    See Ye et al.

  • No. 6 June 2020

    Cryo-FIB milling for structural biology in cells

    3-D segmentation of a cryo-electron tomogram of a cryo-FIB milled human U2OS cell. Various organelles and cytoskeletal elements are shown, e.g., ribosomes in cyan, actin and intermediate filaments in red, microtubules in dark green, mitochondria in light green, nuclear envelope in yellow and nucleoplasm in pink.

    See Wagner et al.

  • No. 5 May 2020

    Confocal image of Neurospora crassahyphae

    Neurospora crassa hyphae expressing CFP (blue) or YFP (yellow). Green are fused hyphae with mixed nuclei in a common cytoplasm. Maximum intensity projection of 3 × 3 tiled z-stack of confocal images. Image is 2.0 × 2.7 mm.

    See: Jonkman et al.

  • No. 4 April 2020

    Tracking single-cell clones using lentiviral barcoding and high-throughput sequencing

    Schematic illustration of the major steps of clonal tracking. From top to bottom: generation of a lentivirus barcode library, barcode integration into the cellular genome, barcode recovery using high-throughput sequencing, and bioinformatic analysis of barcode sequencing data.

    See Bramlett et al.

  • No. 3 March 2020

    Emulating physiological T-cell activation.

    Scanning electron micrograph of a dense cluster of T cells interacting with artificial antigen-presenting cell scaffolds (pseudocolored).

    See Zhang et al.

  • No. 2 February 2020

    E. colion an LP: the power of CRISPR–Cas-mediated genome editing

    This hand-colored scanning electron micrograph shows E. coli living on a phonograph record. These cells were engineered to express molecular components of the CRISPR–Cas system, enabling the cell to write a permanent record of the RNAs within the cell into its own genome, where it is safely stored. Scientists can retrieve the record and reveal the bacterium’s transcriptional history.

    See Tanna et al.

  • No. 1 January 2020

    Reading and writing digital data in DNA.

    To illustrate the capabilities of this protocol the first cover image and the first five articles published in Nature Protocols (Vol. 1, Issue 1, pp 1–29) were stored on 12,474 short DNA sequences and retrieved without error by next-generation sequencing.

    See Meiser et al.