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Clonal tracing in established colon cancer tissue using marker-independent fluorescent labelling
Schematic drawing of red fluorescent clones within established colon cancer tissue and the method to determine the maximum distance (dmax) of cells within a clone, which is used to distinguish individual clones. The numbers in the circles indicate distance in cell diameters.
An important feature of biochemical labeling strategies is their inherent biocompatibility. This protocol enables systematic evaluation of the reaction biocompatibility of (new) synthetic methodologies via bio-additive-based screening.
Here, the authors detail how to fabricate a micrometer-thickness nanoparticulate TiO2-filmed Au ultramicroelectrode and use it for real-time detection of the intrinsic photoelectrochemical behaviors of each individual dye-tagged oxide semiconductor nanoparticle collision associated with a single-entity photoelectrochemical reaction.
This protocol describes how to assemble and characterize 2D plasmonic nanoparticle superlattice sheets. The freestanding nanosheets can be assembled from different nanoparticle building blocks to achieve tailored properties.
This protocol describes the fabrication of nitrogen-vacancy diamond chips, construction of a ‘quantum diamond spectrometer’, and applications for nuclear magnetic resonance (NMR) and electron spin resonance (ESR) spectroscopy in nanoscale volumes.