Volume 13 Issue 9, September 2018

Volume 13 Issue 9

Neural assembloids

A fluorescent image of a 3D human cortical spheroid (hCS) derived from induced pluripotent stem cells that was plated on a glass coverslip and immunostained with antibodies against MAP2 (neurons; green), GFAP (glial lineage cells; purple) and DAPI (nucleiI, blue).

See Sloan et al.

Image: Image taken from the protocol by Sloan et al. Cover Design: Erin Dewalt.

Protocols

  • Protocol |

    Phage immunoprecipitation sequencing (PhIP-Seq) is a method for analyzing antibody-repertoire binding specificities. Phage-displayed oligonucleotide libraries encoding peptidomes are immunoprecipitated and analyzed by high-throughput DNA-Seq.

    • Divya Mohan
    • , Daniel L. Wansley
    • , Brandon M. Sie
    • , Muhammad S. Noon
    • , Alan N. Baer
    • , Uri Laserson
    •  & H. Benjamin Larman
  • Protocol |

    This protocol describes fit-free analysis of fluorescence lifetime imaging microscopy (FLIM) data using the phasor approach. Pixel-by-pixel decays are transformed to the phasor space, and then the clusters can be connected to the image by the reciprocity rules of the phasor plots.

    • Suman Ranjit
    • , Leonel Malacrida
    • , David M. Jameson
    •  & Enrico Gratton
  • Protocol |

    This protocol describes focused ion beam–milling approaches for fabricating oriented single-nanocrystal atomic force microscopy probes, as well as how to measure direction-specific interaction forces between nanocrystals in solution and in vacuum.

    • Xin Zhang
    • , Yang He
    • , Jia Liu
    • , Mark E. Bowden
    • , Libor Kovarik
    • , Scott X. Mao
    • , Chongmin Wang
    • , James J. De Yoreo
    •  & Kevin M. Rosso
  • Protocol |

    This protocol describes how to configure targeted spinal cord stimulation using electromyographic recordings and real-time processing of gait kinematics to enable voluntary control of specific leg movements in rats and nonhuman primates.

    • Marco Capogrosso
    • , Fabien B. Wagner
    • , Jerome Gandar
    • , Eduardo Martin Moraud
    • , Nikolaus Wenger
    • , Tomislav Milekovic
    • , Polina Shkorbatova
    • , Natalia Pavlova
    • , Pavel Musienko
    • , Erwan Bezard
    • , Jocelyne Bloch
    •  & Grégoire Courtine
  • Protocol |

    Neural differentiation and self-organization of hPSCs are induced by culture in suspension. Neural spheroids are then differentiated into dorsal or ventral forebrain spheroids that can be combined to obtain functionally integrated human assembloids.

    • Steven A. Sloan
    • , Jimena Andersen
    • , Anca M. Pașca
    • , Fikri Birey
    •  & Sergiu P. Pașca
  • Protocol |

    Improved and robust procedures for a cyclodextrin-mediated preparation of asymmetric large unilamellar vesicles of diverse lipid compositions and the characterization of their degree of asymmetry and individual leaflet compositions with NMR and GC.

    • Milka Doktorova
    • , Frederick A. Heberle
    • , Barbara Eicher
    • , Robert F. Standaert
    • , John Katsaras
    • , Erwin London
    • , Georg Pabst
    •  & Drew Marquardt
  • Protocol |

    This protocol describes how to use a fully defined, synthetic hydrogel to support the in vitro generation and culture of human organoids derived from pluripotent stem cells and the in vivo delivery of hydrogel-encapsulated organoids into mouse colon.

    • Ricardo Cruz-Acuña
    • , Miguel Quirós
    • , Sha Huang
    • , Dorothée Siuda
    • , Jason R. Spence
    • , Asma Nusrat
    •  & Andrés J. García