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Volume 13 Issue 8, August 2018

Engineering vascularized tissues with AngioChip

A fluorescent image of an AngioChip scaffold with a built-in endothelialized network immunostained for CD31.

See Zhang et al.

Image: Image taken from the protocol by Zhang et al. doi: 10.1038/s41596-018-0015-8. Cover Design: Erin Dewalt.


  • 1,2-diamines are commonly found in pharmaceutically relevant compounds. They can be prepared from diazides. This protocol describes an electrocatalytic method for preparing a wide range of diazides from alkenes, using a manganese catalyst.

    • Niankai Fu
    • Gregory S. Sauer
    • Song Lin


  • This protocol describes a proteomics approach for quantifying newly synthesized proteins (NSPs). NSPs are pulse-labeled with the methionine analog AHA or a heavy-isotope version of it, followed by AHA-peptide enrichment and LC-MS/MS analysis.

    • Yuanhui Ma
    • Daniel B. McClatchy
    • John R. Yates III
  • This protocol describes a pig model in which lungs are transplanted into recipients who are subsequently monitored for 3 d after surgery to study ischemia reperfusion injury and early graft function.

    • Andrea Mariscal
    • Lindsay Caldarone
    • Shaf Keshavjee
  • HyperTRIBE uses a hyperactive RNA-editing enzyme fused to an RNA-binding protein (RBP) to mark the target RNA transcripts of the RBP by converting adenosine to inosine near the binding sites with increased efficiency and reduced sequence bias.

    • Reazur Rahman
    • Weijin Xu
    • Michael Rosbash
  • This protocol describes a noninvasive approach to evoke microglial engulfment and reduce amyloid levels in mouse brain. The authors describe assembly and operation of a light-flicker system, as well as assessment of the molecular and cellular effects.

    • Annabelle C. Singer
    • Anthony J. Martorell
    • Li-Huei Tsai


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