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Farag et al. describe procedures for studying bacterial volatile compound-elicited plant innate immunity and growth promotion. Shown are images of Arabidopsis thaliana seedlings at 24 hours after drop inoculation with the soft-rot pathogen Pectobacterium carotovorum subsp. carotovorum with (left) and without (right) bacterial volatile treatment in a two-compartment Petri plate. Image taken from the protocol by Farag et al. doi:10.1038/nprot.2017.023. Cover design by Jamel Wooten.
In their Perspective, Schubert et al. discuss developments and challenges in mass-spectrometry-based proteomics technology in the past decade and explore its role in molecular systems biology, clinical research and personalized medicine.
The MOSFLM software is widely used for X-ray diffraction data integration. The graphical user interface version, iMosflm, now makes this powerful software program accessible to inexperienced users.
Single-molecule fluorescence in situ hybridization (smFISH) enables mRNA quantification while preserving spatial information. Trcek et al. describe an smFISH procedure for Drosophila embryos, using wide-field, confocal and structured illumination miscroscopy.
Deng et al. describe a method for preparing mechanically responsive hierarchically arranged helical fiber (HHF) actuators based on aligned carbon nanotubes. These HHF actuators are responsive to external stimuli in a rapid and reversible manner.
Bacterial interactions with other species can occur via small, volatile metabolites. In this protocol, volatile metabolites generated by placing two species in a two-Petri-plate system are analyzed by GC–MS after solid-phase microextraction.
This protocol describes how to assess indirectly acquired threat responses in humans. The reaction to the emotional expressions of others is determined by measuring skin conductance responses.
This protocol describes coculture of Caco-2 cells with Raji cells on inverted inserts, resulting in conversion of Caco-2 cells to a microfold (M)-cell-like model for the study of particle, antigen and microorganism translocation in vitro.
This protocol describes how to make SERRS nanoparticles and presents some procedures for using them, including intraoperative, in vivo Raman imaging of cancer in mice.
This protocol describes how to use the paradigm for social transmission of food preference in rats to assess recent and remote associative olfactory memory and underlying processes, including encoding, consolidation, retrieval and forgetting.
Mechanical forces can trigger a variety of biological responses in cells and tissues. This protocol describes how to combine 3D magnetic twisting cytometry with confocal fluorescence microscopy to study these force responses in greater detail.
This protocol describes how to implement a set of rules for robust and quantitative analysis of motor performance in mice. qMotor can be used to assess early disease onset, before paralysis, and disease progression in diverse mouse models, with a smaller number of animals than in previous protocols. qMotor can be used for rapid and robust evaluation of potential therapeutic treatments in diverse mouse disease models.
To quantify transcription factor (TF)–DNA binding dynamics in vivo, this protocol describes how to perform photoactivatable fluorescence correlation spectroscopy in live mouse embryos via selective photoactivation of a subpopulation of TF molecules.
This protocol enables accurate models of the pharmacokinetics and pharmacodynamics of drug action to be determined, using simultaneous dual-channel two-photon fluorescence anisotropy microscopy.
This protocol describes how to present a localized source of Wnt protein to responsive cells in vitro by immobilizing the protein covalently onto synthetic surfaces, mimicking what is often seen in vivo.