Volume 11 Issue 1, January 2016

In this protocol, the construction and use of an operationally simple photochemical microreactor for visible light gas-liquid photoredox catalysis is described. The procedure includes details on how to set up and use the microreactor.

This virus-free approach for mRNA knockdown in vivo uses the siRNA carrier peptide protamine, chemically coupled to a cell surface receptor internalizing antibodies via sulfo-SMCC, to provide protection and guidance for the targeted application of siRNA.

This protocol describes phosphopeptide fractionation for LC-MS/MS analysis using a dual ERLIC-SCX solid-phase extraction method.

This protocol for enhancer scanning to locate regulatory regions in genomic loci enables the identification of candidate functional SNPs within a locus during functional analysis of genome-wide association studies.

This protocol describes assays for assessing heart rate fluctuations in mice or tissue isolated from mice, including by using in vivo telemetry and in vitro electrophysiology of intact sinoatrial network preparations or isolated single sinoatrial node cells.

This protocol describes a Cre-loxP method to measure uptake of extracellular vesicles (EVs). Uptake of EVs released from cells that express Cre recombinase is detected in reporter cell lines as a switch from DsRed to eGFP expression.

This protocol describes PatternLab 4.0, an integrated software environment for the analysis of shotgun proteomics data.

This Protocol describes the CRIS-PITCh and TAL-PITCh systems for MMEJ-based gene targeting using CRISPR-Cas or TALENs. The approach may be particularly useful in systems where HR- or NHEJ-mediated targeting is inefficient.

Viable circulating tumor cells are isolated using easily fabricated microfluidic devices. The hydrodynamic forces present in curvilinear microfluidic channels separate cells on the basis of size.

Metal-organic frameworks (MOFs) are porous, crystalline materials with well-defined structures that can be used in many applications, from gas storage to catalysis and drug storage. This protocol is for the preparation of the MOF NU-1000.

In this Protocol, Barna et al. describe how to use VividSTORM software to correlate the cellular information obtained from confocal imaging with single-molecule localization information obtained from superresolution STORM imaging.

This protocol describes the use of fluorescently quenched activity-based probes (qABPs) that target cysteine cathepsins. This approach allows for tracking of protease activity in tissue samples, which can be used for the detection of tumor resection margins.