Reviews & Analysis

This Perspective explains how click chemistry—specifically, the copper-catalyzed azide–alkyne cycloaddition, the strain-promoted azide–alkyne cycloaddition and the inverse electron-demand Diels–Alder reaction—has revolutionized radiopharmaceutical chemistry.

In this Perspective, authors from the UK Consortium on Metabolic Phenotyping propose a ‘fit-for-purpose’, four-tiered framework to evaluate the reliability of targeted metabolomics analyses, addressing the need for community-accepted, harmonized guidelines for tiers other than full validation.

In this Perspective, the authors propose genetically engineered mouse models to further the understanding and use of a recently discovered tool, selective endogenous encapsidation for cellular delivery via virus-like particles, in gene delivery and gene therapy applications.

This new tutorial from Braun and colleagues details how to design and execute in vivo CRISPR screens in mice to investigate a variety of phenotypes.

Transcranial electrical stimulation (tES) can be used in combination with functional MRI (fMRI) to study brain functions. This Consensus Statement provides a checklist for reporting concurrent tES-fMRI research.

Cue reactivity measured by functional magnetic resonance imaging is used in studies of substance-use disorders. This Consensus Statement is the result of a Delphi process to arrive at parameters that should be reported in describing these studies.

This review explores factors to consider when introducing electronic laboratory notebooks, including discussion of integration with research data management and the functionalities to compare when evaluating specific software packages.

This Perspective discusses the use of CRISPR–Cas9-mediated gene conversion systems in laboratory and wild rodents. Although technical hurdles must be overcome before wild-release strategies are feasible, modest improvements could increase the efficiency and reduce the cost of mouse genetics in the laboratory.

The authors describe three-dimensional imaging pipelines available to analyze archival patient specimens. The pipelines facilitate the visualization of both large and small volumes of tissue with subcellular resolution.

This tutorial describes how to develop a nanoinjection workflow, including the selection of nanoneedle devices, approaches to loading cargo, strategies for interfacing to biological systems and assays to evaluate the efficacy of intracellular delivery.

Mass-spectrometry-based proteomics is a powerful approach for discovering disease biomarkers. This tutorial provides advice on the study design, including cohort selection, evaluating statistical power, blinding and randomization, and quality control.

Understanding the heterogeneity of extracellular vesicles is crucial for unraveling their functions. This review describes the benefits, challenges and contributions of the state-of-the art methods used in single-vesicle analysis.

This tutorial provides guidelines for interpreting single-cell transcriptomic maps to identify cell types, states and other biologically relevant patterns.

This tutorial provides guidance for selecting and optimizing tissue-clearing protocols for specific samples and biological questions. In addition, instructions are provided for developing an imaging strategy and processing the resulting data.

This Perspective from the Harvard Chan Microbiome in Public Health Center describes a generalizable and scalable approach to stool and oral microbiome and metadata collection in the Micro-N (Microbiome Among Nurses) study, to show how to carry out prospective studies of the microbiome.

This tutorial explains how to evaluate and benchmark metagenome assembly, binning and profiling methods using standards and software provided by the CAMI initiative.

In this review article, the authors compare in vitro cytotoxicity assays (including chromium release, bioluminescence, impedance and flow cytometry assays) with respect to their experimental setup, appropriate uses, advantages and disadvantages and measures to overcome their limitations.

This review illustrates the power of on-resin multicomponent reaction protocols to create complexity and diversity in biomolecular skeletons such as peptides and oligonucleotides, including strategies to assemble, label and conjugate them.

In this Tutorial Review, Hemberg et al. present an overview of the computational workflow involved in processing single-cell RNA sequencing data.

Off-target effects of programmable nucleases remain a critical issue for therapeutic applications of genome editing. This review compares experimental and computational tools for off-target analysis and provides recommendations for better assessments of off-target effects.