Volume 13 Issue 4, April 2018

Optically active compounds can be prepared using chiral catalysts. This protocol describes rhodium- and iridium-based asymmetric catalysts that rely exclusively on metal-centered chirality. Applications to asymmetric photoredox catalysis are provided.

This protocol describes XCMS Online, a web-based systems biology platform for processing of metabolomics data, pathway mapping and integration with genomics and proteomics data.

This protocol describes the procedures to label RNA molecules for ¹⁹F NMR in order to study RNA G-quadruplex structures in vitro and in living Xenopus laevis oocyte cells.

In structure-based drug design, scoring functions are used to evaluate protein–ligand interactions. This protocol shows how to use the CASF-2013 benchmark to assess scoring functions in terms of their scoring, ranking, docking, and screening power.

This protocol describes a series of procedures to produce a variety of piezoelectric PVDF-based structures for a broad range of applications in biomedicine, sensors and actuators, energy generation and storage, and filtration membranes, among others.

This biologist-friendly modeling scheme facilitates the capture and visualization of knowledge about biological pathways and how components interact. These pathway models can be directly used to run simulations of their activity and test hypotheses.

This protocol describes the generation of early-developing cardiac organoids from human pluripotent stem cells. Geometric confinement of the hiPSCs drives spatial organization of the cells from a 2D layer into 3D cardiac microchambers.

This protocol describes how to isolate endothelial cells and pericytes from adult mouse brain microregions using fluorescence-activated cell sorting without requirement for transgenic mice. The cells may be used for diverse downstream applications.

This protocol describes the identification of in vivo target interaction profiles of covalent enzyme inhibitors using chemical proteomics. Enzymes are enriched by activity-based probes and quantified by label-free mass spectrometry.

This protocol describes a method to sequence small RNAs by target enrichment (TEsR). By enriching for sRNAs, TEsR allows the quantitative detection of not only small RNAs but also their precursors and intermediate and mature forms.

This protocol describes SHIMS 2.0, a method to generate accurate reference sequences from structurally complex genomic regions in a cost-effective, high-throughput manner.