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This Protocol Extension describes how to prepare plant tissue to enable Spatial Transcriptomics profiling. Spatial Transcriptomics is achieved through the combination of histological staining of the plant tissue with spatially resolved RNA sequencing.
In this protocol extension, the authors detail an in-cell version of their previous in vitro SHAPE-MaP protocol, enabling RNA structure to be probed in living cells.
This protocol describes production and bioinformatics analysis pipelines for E/L Repli-seq, an extension of the earlier Repli-chip protocol, allowing rapid genome-wide replication-timing analysis by next-generation sequencing.
This protocol extension describes DNA-free genome editing of bread wheat by delivering in vitro transcripts (IVTs) or ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 by particle bombardment. The authors' previously published protocol for genome editing of wheat used CRISPR/Cas9 plasmids.