In this protocol update, the authors improve on their previous protocol for genome engineering of mammalian cultured cells with CRISPR–Cas9 to generate homozygous knock-ins of fluorescent tags into endogenous genes, to increase both efficiency and throughput.

Surface tension, interfacial tension and interfacial rheological properties can be calculated from droplet images. This article presents a standardized protocol for pendant-drop tensiometry and the oscillating drop method in two- and three-phase systems.

We present a practical workflow for end-to-end weakly supervised deep learning to predict biomarkers directly from whole-slide images, enabling clinical researchers to work with engineers to set up a complete computational pathology project.

CellChat enables systematic inference, quantitative analysis and intuitive visualization of cell–cell communication from single-cell transcriptomic data, as well as comparative analysis of intercellular communication across biological conditions.

An approach that combines a pH-sensitive synaptic biomarker expressed in vivo with the ex vivo staining of oligodendrocyte precursor cells enables quantification of synapse engulfment by oligodendrocyte precursor cells at single-cell and population-level resolution.

MINUTE-ChIP is a multiplexed chromatin immunoprecipitation and sequencing method that measures global and locus-specific changes in histone modification patterns and chromatin factor binding across multiple samples and conditions.

This protocol describes a method for haplotype phasing plant genomes, using gamete cells to enable chromosome-level phasing and crossover detection without the need for Hi-C data or sequencing of large plant populations.

This protocol presents a method for calibrating circular RNA abundance for comparison between RNA sequencing libraries, utilizing a spike-in of synthetic circular RNAs.

This protocol presents a method that combines genome-wide CRISPR libraries with cell coculture in droplets to study functional regulators of cell–cell communication.

Most naturally occurring O-GalNAc glycans can be synthesized from eight glycan core structures. This protocol describes their synthesis from a common precursor via reactions with four glycosyl donors.

In this protocol update, the authors improve on their previous protocol for genome engineering of mammalian cultured cells with CRISPR–Cas9 to generate homozygous knock-ins of fluorescent tags into endogenous genes, to increase both efficiency and throughput.

Feature-based molecular networking is used to analyze non-targeted liquid chromatography–tandem mass spectrometry metabolomics data. This protocol includes instructions, ready-made code and a web app (https://fbmn-statsguide.gnps2.org/) for statistical analysis of feature-based molecular networking results.

Surface tension, interfacial tension and interfacial rheological properties can be calculated from droplet images. This article presents a standardized protocol for pendant-drop tensiometry and the oscillating drop method in two- and three-phase systems.