Angstrom-scale two-dimensional channel devices can be assembled with precise control over their dimensions, as a single channel or hundreds of channels using layered crystals, and enable the measurement of angstrom-scale gas, ion and water fluidics.

Single-cell multiple displacement amplification with the variant caller SCcaller ensures high-fidelity, quantitative analysis of single-nucleotide variations and small insertions and deletions.

This protocol describes procedures for learning cellular and molecular processes from single-cell RNA-sequencing data using the non-negative matrix factorization algorithm Coordinated Gene Activity across Pattern Subsets. Parallel analysis is demonstrated in Python, R and GenePattern Notebook.

Mitochondrial protein assemblies are vital for neuronal and brain function. This protocol describes a co-fractionation–mass spectrometry platform to study native motochondrial assemblies in brain and cultured nerve cells after chemical cross-linking.

Two-dimensional protein films, prepared via amyloid-like aggregation, can be made with nano- to meter-scale dimensions and features such as high interface adhesion, as well as tunable antibacterial, biomineralization and antifouling activity.

Tail-to-head terpene cyclization is an essential reaction for terpene synthesis, but it is difficult to achieve without an enzyme. This protocol describes the preparation and use of a hexameric resorcin[4]arene capsule as an artificial enzyme.

This protocol for the spatiotemporal control of RNA activity uses LicV, a synthetic, photoswitchable RNA-binding protein (RBP) that can bind to a specific RNA sequence in response to blue light irradiation, and provides an efficient and generalizable strategy for engineering photoswitchable RBPs.

This protocol describes a reproducible and reliable method for excisional skin wound healing assays in mice. The use of lineage-tracing assays to investigate the contribution of different cell populations to the repair process is also discussed.

This protocol outlines a method for introgressing the entire genome of the wild Aegilops tauschii into elite wheat cultivars within a period of 2 y.

CONIPHER is a computational framework for accurately inferring subclonal structure and the phylogenetic tree from multisample tumor sequencing, accounting for both copy number alterations and mutation errors.

This protocol describes barcoding bacteria for identification and quantification, a method for identifying and quantifying bacterial cells in microbiota samples based on the droplet-based barcoding and amplification of 16S rRNA genes from single bacterial cells.

Angstrom-scale two-dimensional channel devices can be assembled with precise control over their dimensions, as a single channel or hundreds of channels using layered crystals, and enable the measurement of angstrom-scale gas, ion and water fluidics.

Obtaining large, uniform crystals of polymer materials, including covalent organic frameworks, is challenging. This protocol describes a fast, effective approach to making crystalline two- and three-dimensional covalent organic frameworks using supercritical CO₂ as the solvent.