This Protocol documents the extensive preparation required to adopt Neuropixels probes for use in patients for recording spiking activity from up to hundreds of cortical neurons and associating it with local field potentials.

We provide a Perspective highlighting the significant ethical implications of the use of fast-developing neurotechnologies in humans, as well as the regulatory frameworks and guidelines needed to protect neurodata and mental privacy.

A correlative imaging approach in which matrix-assisted laser desorption ionization mass spectrometry imaging is followed by fluorescence in situ hybridization (metaFISH) for the spatial metabolomics identification of host–microbiome interactions, at single-cell resolution, via nucleic acid probes.

A simple and robust approach for the large-scale assembly of DNA-templated precise carbon nanotube semiconductor arrays with clean channel interfaces to build field-effect transistors for high-performance electronics.

This Tutorial provides guide for how to evaluate, select and use publicly available computational tools for predicting intrinsic disorder in proteins, with a focus on performance and ease of use results, exemplified using results from the Critical Assessment of protein Intrinsic Disorder prediction experiment.

This protocol provides structure-guided capsid library approaches to evolve new adeno-associated viral vectors for enhanced CNS gene delivery with altered tissue tropism, higher transduction efficiency and the ability to evade pre-existing humoral immunity.

This protocol describes how to prepare ordered mesoporous intermetallic nanomaterials with controlled compositions, morphologies/structures and phases via a general concurrent template strategy.

Purifying proteins by crystallization rather than chromatography is less expensive and more environmentally sustainable. This protocol describes how to determine the operational conditions for purifying monoclonal antibodies by template-assisted membrane crystallization.

This Protocol documents the extensive preparation required to adopt Neuropixels probes for use in patients for recording spiking activity from up to hundreds of cortical neurons and associating it with local field potentials.

This protocol presents a workflow for the enrichment of plant nuclei from cells targeted by pathogenic bacterial effectors during bacterial infection.

We provide a Perspective highlighting the significant ethical implications of the use of fast-developing neurotechnologies in humans, as well as the regulatory frameworks and guidelines needed to protect neurodata and mental privacy.

Immobilization of enzymes in solid porous supports can enhance the recyclability and stability of enzymes. This protocol describes direct-immobilization methods to make enzyme-covalent organic framework biocomposites.

A correlative imaging approach in which matrix-assisted laser desorption ionization mass spectrometry imaging is followed by fluorescence in situ hybridization (metaFISH) for the spatial metabolomics identification of host–microbiome interactions, at single-cell resolution, via nucleic acid probes.