This protocol enables the generation of human forebrain organoids from induced pluripotent stem cells with the intrinsic ability to assemble functionally integrated bilateral optic vesicles.

We present the design, assembly and implementation of a molecular-electromechanical system composed of a tetrahedral DNA nanostructure immobilized on a graphene field-effect transistor for unamplified detection of trace amounts of analytes in biofluids.

A protocol for the directed differentiation of human pluripotent stem cells into distal lung organoids that can be used to model interstitial lung disease, viral infection and human lung development, along with procedures for their analysis.

This protocol for Multiplexed Intermixed CRISPR Droplets uses microfluidics to create droplets containing Cas9, multiplexed single-guide RNAs and corresponding DNA barcodes, allowing large-scale genetic screens to be performed in F0 zebrafish.

A step-by-step guide providing biomedical researchers instructions for implementing spatial- and Fourier-domain ptychography to existing setups with minimal hardware modifications.

The authors describe the fabrication, assembly and long-term implantation of ultraflexible neural probes for the simultaneous recording of neural activity and neuromodulation in rodents.

A protocol for the generation of medulloblastoma and high-grade glioma organoids by electroporating, respectively, cerebellum and forebrain organoids, along with procedures for their use in drug screening, lineage tracing, co-culture and in vivo assays.

Cellulose is a burgeoning feedstock for more sustainable alternatives to fossil fuel-based materials. This protocol uses a cellulose-dissolving ionic liquid electrolyte with nuclear magnetic resonance methods to provide high-resolution structural analysis of cellulose.

The authors describe spiral volumetric optoacoustic tomography for the visualization of contrast agent perfusion and biodistribution in mice, featuring excellent scalability to achieve spatial resolution down to 90 µm or whole-body scans in <2 s.

We present the design, assembly and implementation of a molecular-electromechanical system composed of a tetrahedral DNA nanostructure immobilized on a graphene field-effect transistor for unamplified detection of trace amounts of analytes in biofluids.

We present a protocol for the encapsulation and stabilization of enzymes by using hydrogen-bonded organic frameworks (HOFs)—enzyme@HOF-101—along with procedures for their material characterization and testing of their biocatalytic performance.

This protocol enables the generation of human forebrain organoids from induced pluripotent stem cells with the intrinsic ability to assemble functionally integrated bilateral optic vesicles.

AbEMap generates large ensembles of docked antigen–antibody structures based on the structure of an antigen and either the structure or the sequence of an antibody. For each antigen residue, a likelihood score for being part of the epitope is obtained.