Volume 3 Issue 10, October 2018

Volume 3 Issue 10

Lost in hibernation

Cryo-electron-microscopy imaging of hibernating ribosomes from Escherichia coli elucidates the molecular composition of these complexes and their mode of assembly, reveals how translation initiation is inhibited, and identifies a role for the ribosomal protein S1 in ribosome inactivation.

See Beckert et al.

Image: Bertrand Beckert, Martin Turk, Daniel N. Wilson. Cover Design: Samantha Whitham.

Editorial

  • Editorial |

    Without greater attention being paid to the work–life balance of academic researchers, effects on creativity, productivity, interpersonal relationships and mental health will continue to grow, increasing the risk of burnout.

News & Views

  • News & Views |

    The H445Y mutation in the β-subunit of RNA polymerase in Mycobacterium tuberculosis causes resistance to the antibiotic rifampicin and changes mycobacterial lipid composition. Infection with rpoB H445Y results in metabolic shifts that allow the bacteria to bypass a key host inflammatory response.

    • Yu-Xiang Chen
    •  & Babak Javid
  • News & Views |

    How the immune system handles the relentless presence of commensal bacteria is an area of great interest. Here, researchers describe a role for autophagy in mediating tolerance to the microbiota, the absence of which can impart beneficial resistance to infection but also possible detriment in the form of autoimmunity.

    • Jennifer Martinez
  • News & Views |

    Components of the sphingolipid biosynthetic pathway induce a favourable conformation in the proteinaceous murine norovirus receptor (CD300lf) that facilitates virus entry.

    • Benhur Lee

Research

  • Brief Communication |

    Genomic characterization of a new ebolavirus, detected in free-tailed bats in Sierra Leone, whose viral glycoprotein can mediate entry into human cells.

    • Tracey Goldstein
    • , Simon J. Anthony
    • , Aiah Gbakima
    • , Brian H. Bird
    • , James Bangura
    • , Alexandre Tremeau-Bravard
    • , Manjunatha N. Belaganahalli
    • , Heather L. Wells
    • , Jasjeet K. Dhanota
    • , Eliza Liang
    • , Michael Grodus
    • , Rohit K. Jangra
    • , Veronica A. DeJesus
    • , Gorka Lasso
    • , Brett R. Smith
    • , Amara Jambai
    • , Brima O. Kamara
    • , Sorie Kamara
    • , William Bangura
    • , Corina Monagin
    • , Sagi Shapira
    • , Christine K. Johnson
    • , Karen Saylors
    • , Edward M. Rubin
    • , Kartik Chandran
    • , W. Ian Lipkin
    •  & Jonna A. K. Mazet
  • Letter |

    A combination of regulated gene expression, mutagenesis and electrophysiology experiments shows that EXP2, in addition to its role as part of the Plasmodium translocon of exported proteins (PTEX), functions as a nutrient-permeable channel in the vacuolar membrane of the parasite.

    • Matthias Garten
    • , Armiyaw S. Nasamu
    • , Jacquin C. Niles
    • , Joshua Zimmerberg
    • , Daniel E. Goldberg
    •  & Josh R. Beck
  • Letter |

    A prevalent rifampicin resistance mutation in Mycobacterium tuberculosis alters bacterial virulence lipid expression and enables bypasses of a host immune axis that is critical for the control of drug-susceptible infections.

    • Nicole C. Howard
    • , Nancy D. Marin
    • , Mushtaq Ahmed
    • , Bruce A. Rosa
    • , John Martin
    • , Monika Bambouskova
    • , Alexey Sergushichev
    • , Ekaterina Loginicheva
    • , Natalia Kurepina
    • , Javier Rangel-Moreno
    • , Liang Chen
    • , Barry N. Kreiswirth
    • , Robyn S. Klein
    • , Joan-Miquel Balada-Llasat
    • , Jordi B. Torrelles
    • , Gaya K. Amarasinghe
    • , Makedonka Mitreva
    • , Maxim N. Artyomov
    • , Fong-Fu Hsu
    • , Barun Mathema
    •  & Shabaana A. Khader
  • Letter |

    Cryo-electron-microscopy imaging of hibernating ribosomes from Escherichia coli elucidates the molecular composition of these complexes and their mode of assembly, reveals how translation initiation is inhibited, and identifies a role for the ribosomal protein S1 in ribosome inactivation.

    • Bertrand Beckert
    • , Martin Turk
    • , Andreas Czech
    • , Otto Berninghausen
    • , Roland Beckmann
    • , Zoya Ignatova
    • , Jürgen M. Plitzko
    •  & Daniel N. Wilson
  • Article |

    Autophagy-deficient murine hosts are protected from infection and injury at the intestinal barrier and this is driven by a microbiota-dependent type I interferon response, indicating that autophagy is important for limiting host responses to the microbiota.

    • Patricia K. Martin
    • , Amanda Marchiando
    • , Ruliang Xu
    • , Eugene Rudensky
    • , Frank Yeung
    • , Samantha L. Schuster
    • , Elisabeth Kernbauer
    •  & Ken Cadwell
  • Article |

    A combination of cryo-EM structures and experiments using liposomes and lipid nanodiscs elucidates how the Tse6 effector is loaded into the type VI secretion system and travels across membranes to intoxicate target cells.

    • Dennis Quentin
    • , Shehryar Ahmad
    • , Premy Shanthamoorthy
    • , Joseph D. Mougous
    • , John C. Whitney
    •  & Stefan Raunser
  • Article |

    Male offspring of pregnant mice infected with a low dose of Zika virus infection have increased testosterone levels, an increased number of immature neurons in apical hippocampal dendrites and are less likely to survive in utero infection than female littermates.

    • Stephanie Stanelle-Bertram
    • , Kerstin Walendy-Gnirß
    • , Thomas Speiseder
    • , Swantje Thiele
    • , Ivy Asantewaa Asante
    • , Carola Dreier
    • , Nancy Mounogou Kouassi
    • , Annette Preuß
    • , Gundula Pilnitz-Stolze
    • , Ursula Müller
    • , Stefanie Thanisch
    • , Melanie Richter
    • , Robin Scharrenberg
    • , Vanessa Kraus
    • , Ronja Dörk
    • , Lynn Schau
    • , Vanessa Herder
    • , Ingo Gerhauser
    • , Vanessa Maria Pfankuche
    • , Christopher Käufer
    • , Inken Waltl
    • , Thais Moraes
    • , Julie Sellau
    • , Stefan Hoenow
    • , Jonas Schmidt-Chanasit
    • , Stephanie Jansen
    • , Benjamin Schattling
    • , Harald Ittrich
    • , Udo Bartsch
    • , Thomas Renné
    • , Ralf Bartenschlager
    • , Petra Arck
    • , Daniel Cadar
    • , Manuel A. Friese
    • , Olli Vapalahti
    • , Hanna Lotter
    • , Sany Benites
    • , Lane Rolling
    • , Martin Gabriel
    • , Wolfgang Baumgärtner
    • , Fabio Morellini
    • , Sabine M. Hölter
    • , Oana Amarie
    • , Helmut Fuchs
    • , Martin Hrabe de Angelis
    • , Wolfgang Löscher
    • , Froylan Calderon de Anda
    •  & Gülsah Gabriel
  • Article |

    Genomic analysis uncovers global prevalence of three multidrug-resistant Staphylococcus epidermidis lineages encoding rifampicin resistance and reduced susceptibility to glycopeptide antibiotics.

    • Jean Y. H. Lee
    • , Ian R. Monk
    • , Anders Gonçalves da Silva
    • , Torsten Seemann
    • , Kyra Y. L. Chua
    • , Angela Kearns
    • , Robert Hill
    • , Neil Woodford
    • , Mette D. Bartels
    • , Birgit Strommenger
    • , Frederic Laurent
    • , Magali Dodémont
    • , Ariane Deplano
    • , Robin Patel
    • , Anders R. Larsen
    • , Tony M. Korman
    • , Timothy P. Stinear
    •  & Benjamin P. Howden

Amendments & Corrections

  • Author Correction |

    • Patrick Munk
    • , Berith Elkær Knudsen
    • , Oksana Lukjancenko
    • , Ana Sofia Ribeiro Duarte
    • , Liese Van Gompel
    • , Roosmarijn E. C. Luiken
    • , Lidwien A. M. Smit
    • , Heike Schmitt
    • , Alejandro Dorado Garcia
    • , Rasmus Borup Hansen
    • , Thomas Nordahl Petersen
    • , Alex Bossers
    • , Etienne Ruppé
    • , Ole Lund
    • , Tine Hald
    • , Sünje Johanna Pamp
    • , Håkan Vigre
    • , Dick Heederik
    • , Jaap A. Wagenaar
    • , Dik Mevius
    • , Frank M. Aarestrup
    • , Haitske Graveland
    • , Alieda van Essen
    • , Bruno Gonzalez-Zorn
    • , Gabriel Moyano
    • , Pascal Sanders
    • , Claire Chauvin
    • , Julie David
    • , Antonio Battisti
    • , Andrea Caprioli
    • , Jeroen Dewulf
    • , Thomas Blaha
    • , Katharina Wadepohl
    • , Maximiliane Brandt
    • , Dariusz Wasyl
    • , Magdalena Skarzyńska
    • , Magdalena Zajac
    • , Hristo Daskalov
    • , Helmut W. Saatkamp
    •  & Katharina D. C. Stärk
  • Publisher Correction |

    • Angelino T. Tromp
    • , Michiel Van Gent
    • , Pauline Abrial
    • , Amandine Martin
    • , Joris P. Jansen
    • , Carla J. C. De Haas
    • , Kok P. M. Van Kessel
    • , Bart W. Bardoel
    • , Elisabeth Kruse
    • , Emilie Bourdonnay
    • , Michael Boettcher
    • , Michael T. McManus
    • , Christopher J. Day
    • , Michael P. Jennings
    • , Gérard Lina
    • , François Vandenesch
    • , Jos A. G. Van Strijp
    • , Robert Jan Lebbink
    • , Pieter-Jan A. Haas
    • , Thomas Henry
    •  & András N. Spaan