Volume 2 Issue 5, May 2017

Volume 2 Issue 5

Cycle proficiency

In the pathogenic blood stage of infection, Plasmodium falciparum cell-cycle progression is directed by the parasite-specific kinase CRK4. Without active CRK4, DNA replication is inhibited and the nuclei are deformed by spindle-like structures.

See Ganter et al. 2, 17017 (2017)

Image: Markus Ganter, Cover Design: Samantha Whitham

Editorial

  • Editorial |

    If we are to be successful in keeping priority pathogens at bay in the long term, global responses to antimicrobial resistance should embrace and fund innovative therapeutic strategies that are developed in the basic microbiology laboratory.

Comment and Opinion

News and Views

  • News & Views |

    Bacterial specialized metabolites are bioactive molecules with antibacterial or other activities that are of tremendous clinical use. New work has revealed that transcript elongation is a distinct and widespread point of secondary metabolic gene regulation, which has implications for expanding drug discovery.

    • Justin R. Nodwell
  • News & Views |

    The sensor cyclic GMP–AMP synthase (cGAS) is well known to recognize viral DNA. In this issue of Nature Microbiology, infection by dengue virus (DENV), which has an RNA genome, is shown to induce mitochondrial DNA release into the cytosol, leading to cGAS activation. In turn, DENV targets cGAS to evade antiviral immunity.

    • Michiel van Gent
    •  & Michaela U. Gack

Review and Perspective

Research

  • Article |

    This study identifies regulators of the RNA sensor RIG-I. Diverse processes, including the unfolded protein response, Wnt signalling and RNA metabolism, modulate this pathway. The splicing regulator KHSRP is identified as a negative regulator of RIG-I that affects influenza replication in vivo.

    • Stephen Soonthornvacharin
    • , Ariel Rodriguez-Frandsen
    • , Yingyao Zhou
    • , Felipe Galvez
    • , Nicholas J. Huffmaster
    • , Shashank Tripathi
    • , Vinod R. M. T. Balasubramaniam
    • , Atsushi Inoue
    • , Elisa de Castro
    • , Hong Moulton
    • , David A. Stein
    • , María Teresa Sánchez-Aparicio
    • , Paul D. De Jesus
    • , Quy Nguyen
    • , Renate König
    • , Nevan J. Krogan
    • , Adolfo García-Sastre
    • , Sunnie M. Yoh
    •  & Sumit K. Chanda
  • Article |

    The gut membrane-associated protein Mesh controls proliferation of gut bacteria by regulating dual-oxidase expression through an arrestin-mediated MAPK JNK/ERK phosphorylation cascade in Aedes aegypti and Drosophila melanogaster.

    • Xiaoping Xiao
    • , Lijuan Yang
    • , Xiaojing Pang
    • , Rudian Zhang
    • , Yibin Zhu
    • , Penghua Wang
    • , Guanjun Gao
    •  & Gong Cheng
  • Article |

    The long-term dynamic behaviour of the gut microbiome in inflammatory bowel disease demonstrates increased deviation from the ‘healthy plane’ when compared to the normal variation observed in healthy individuals.

    • Jonas Halfvarson
    • , Colin J. Brislawn
    • , Regina Lamendella
    • , Yoshiki Vázquez-Baeza
    • , William A. Walters
    • , Lisa M. Bramer
    • , Mauro D'Amato
    • , Ferdinando Bonfiglio
    • , Daniel McDonald
    • , Antonio Gonzalez
    • , Erin E. McClure
    • , Mitchell F. Dunklebarger
    • , Rob Knight
    •  & Janet K. Jansson
  • Article |

    This study shows that mitochondrial DNA leaks into the cytoplasm during dengue virus infection, activating the DNA sensor cGAS. Viral NS2B targets cGAS for lysosomal degradation, inhibiting type I interferon responses in infected cells.

    • Sebastian Aguirre
    • , Priya Luthra
    • , Maria T. Sanchez-Aparicio
    • , Ana M. Maestre
    • , Jenish Patel
    • , Francise Lamothe
    • , Anthony C. Fredericks
    • , Shashank Tripathi
    • , Tongtong Zhu
    • , Jessica Pintado-Silva
    • , Laurence G. Webb
    • , Dabeiba Bernal-Rubio
    • , Alexander Solovyov
    • , Benjamin Greenbaum
    • , Viviana Simon
    • , Christopher F. Basler
    • , Lubbertus C. F. Mulder
    • , Adolfo García-Sastre
    •  & Ana Fernandez-Sesma
  • Article |

    Analysis and annotation of the Listeria monocytogenes proteome identifies a miniprotein that is required for the bacterial stressosome response.

    • Francis Impens
    • , Nathalie Rolhion
    • , Lilliana Radoshevich
    • , Christophe Bécavin
    • , Mélodie Duval
    • , Jeffrey Mellin
    • , Francisco García del Portillo
    • , M. Graciela Pucciarelli
    • , Allison H. Williams
    •  & Pascale Cossart
  • Letter |

    Marine heterotrophic bacteria produce the signalling molecule dimethylsulfoniopropionate (DMSP) via the methyltransferase gene dsyB, implicating bacteria as contributors to the oceanic DMSP pool, in addition to macroalgae and phytoplankton.

    • Andrew R. J. Curson
    • , Ji Liu
    • , Ana Bermejo Martínez
    • , Robert T. Green
    • , Yohan Chan
    • , Ornella Carrión
    • , Beth T. Williams
    • , Sheng-Hui Zhang
    • , Gui-Peng Yang
    • , Philip C. Bulman Page
    • , Xiao-Hua Zhang
    •  & Jonathan D. Todd
  • Letter |

    A screen for outer-membrane permeating compounds finds that the clinically approved antiprotozoal drug pentamidine sensitizes drug-resistant Gram-negative pathogens to new antibiotic classes.

    • Jonathan M. Stokes
    • , Craig R. MacNair
    • , Bushra Ilyas
    • , Shawn French
    • , Jean-Philippe Côté
    • , Catrien Bouwman
    • , Maya A. Farha
    • , Arthur O. Sieron
    • , Chris Whitfield
    • , Brian K. Coombes
    •  & Eric D. Brown
  • Letter |

    Plasmodium falciparum kinase PfCRK4 is a key regulator of DNA replication in schizonts, required both during the intraerythrocytic blood stage of malaria infection and for transmission.

    • Markus Ganter
    • , Jonathan M. Goldberg
    • , Jeffrey D. Dvorin
    • , Joao A. Paulo
    • , Jonas G. King
    • , Abhai K. Tripathi
    • , Aditya S. Paul
    • , Jing Yang
    • , Isabelle Coppens
    • , Rays H. Y. Jiang
    • , Brendan Elsworth
    • , David A. Baker
    • , Rhoel R. Dinglasan
    • , Steven P. Gygi
    •  & Manoj T. Duraisingh
  • Letter |

    Rice dwarf virus capsid protein binds to the outer membrane protein of the obligate symbiont bacterium Sulcia, allowing the virus to hijack the oocyte entry path in rice leafhopper vectors for transovarial transmission.

    • Dongsheng Jia
    • , Qianzhuo Mao
    • , Yong Chen
    • , Yuyan Liu
    • , Qian Chen
    • , Wei Wu
    • , Xiaofeng Zhang
    • , Hongyan Chen
    • , Yi Li
    •  & Taiyun Wei

Amendments and Corrections