Latest Research

  • Article |

    Levulinic acid (LA) is a value-added chemical easily obtained from biomass. The pathway enabling LA degradation in Pseudomonas putida requires five enzymes and can be engineered into Escherichia coli, which could enable further biotechnological applications.

    • Jacqueline M. Rand
    • , Tippapha Pisithkul
    • , Ryan L. Clark
    • , Joshua M. Thiede
    • , Christopher R. Mehrer
    • , Daniel E. Agnew
    • , Candace E. Campbell
    • , Andrew L. Markley
    • , Morgan N. Price
    • , Jayashree Ray
    • , Kelly M. Wetmore
    • , Yumi Suh
    • , Adam P. Arkin
    • , Adam M. Deutschbauer
    • , Daniel Amador-Noguez
    •  & Brian F. Pfleger
  • Letter |

    Reactive oxygen species, most likely spawned from dietary-induced fatty acid β-oxidation, directly impact Plasmodium survival inside hepatocytes and sigificantly reduce liver infection.

    • Vanessa Zuzarte-Luís
    • , João Mello-Vieira
    • , Inês M. Marreiros
    • , Peter Liehl
    • , Ângelo F. Chora
    • , Céline K. Carret
    • , Tânia Carvalho
    •  & Maria M. Mota
  • Letter |

    The structure of the extended sheath–tube complex of the type VI secretion system from Vibrio cholerae elucidates the molecular mechanisms by which conformational changes in the sheath enable the inner tube to penetrate target cells.The structure of the extended sheath–tube complex of the type VI secretion system from Vibrio cholerae elucidates the molecular mechanisms by which conformational changes in the sheath enable the inner tube to penetrate target cells.

    • Jing Wang
    • , Maximilian Brackmann
    • , Daniel Castaño-Díez
    • , Mikhail Kudryashev
    • , Kenneth N. Goldie
    • , Timm Maier
    • , Henning Stahlberg
    •  & Marek Basler
  • Letter |

    Streptococcal para-amino benzoic acid enhances Porphyromonas gingivalis colonization while reducing virulence during polymicrobial oral infection.

    • Masae Kuboniwa
    • , John R. Houser
    • , Erik L. Hendrickson
    • , Qian Wang
    • , Samar A. Alghamdi
    • , Akito Sakanaka
    • , Daniel P. Miller
    • , Justin A. Hutcherson
    • , Tiansong Wang
    • , David A. C. Beck
    • , Marvin Whiteley
    • , Atsuo Amano
    • , Huizhi Wang
    • , Edward M. Marcotte
    • , Murray Hackett
    •  & Richard J. Lamont
  • Letter |

    Infection of the alga Emiliania huxleyi with its virus EhV results in the increased release of extracellular vesicles that impact viral decay and infection, suggesting that EhV exploits these extracellular vesicles for efficient viral infection during algal blooms.

    • Daniella Schatz
    • , Shilo Rosenwasser
    • , Sergey Malitsky
    • , Sharon G. Wolf
    • , Ester Feldmesser
    •  & Assaf Vardi
  • Letter |

    Recombinant proteins based on APOL1 and APOL3 can kill pathogenic Trypanosoma brucei subspecies, including a variant (rPpMUT) that is effective against T.b. gambiense infection in mice, suggesting that it may serve as a therapy against sleeping sickness.

    • Frédéric Fontaine
    • , Laurence Lecordier
    • , Gilles Vanwalleghem
    • , Pierrick Uzureau
    • , Nick Van Reet
    • , Martina Fontaine
    • , Patricia Tebabi
    • , Benoit Vanhollebeke
    • , Philippe Büscher
    • , David Pérez-Morga
    •  & Etienne Pays
  • Article | | open

    The recovery of 7,903 bacterial and archaeal metagenome-assembled genomes increases the phylogenetic diversity represented by public genome repositories and provides the first representatives from 20 candidate phyla.

    • Donovan H. Parks
    • , Christian Rinke
    • , Maria Chuvochina
    • , Pierre-Alain Chaumeil
    • , Ben J. Woodcroft
    • , Paul N. Evans
    • , Philip Hugenholtz
    •  & Gene W. Tyson
  • Article |

    The structures of a variant surface glycoprotein (VSG) from Trypanosoma brucei suggest that VSGs adopt different conformations to respond to obstacles present in the cell membrane, enabling them to maintain a protective coat at all times.

    • Thomas Bartossek
    • , Nicola G. Jones
    • , Christin Schäfer
    • , Mislav Cvitković
    • , Marius Glogger
    • , Helen R. Mott
    • , Jochen Kuper
    • , Martha Brennich
    • , Mark Carrington
    • , Ana-Sunčana Smith
    • , Susanne Fenz
    • , Caroline Kisker
    •  & Markus Engstler
  • Letter |

    LysM, the lysis protein of the Escherichia coli levivirus M, represents a new ‘protein antibiotic’ that interferes with the synthesis of peptidoglycan by inhibiting lipid II flipping.

    • Karthik R. Chamakura
    • , Lok-To Sham
    • , Rebecca M. Davis
    • , Lorna Min
    • , Hongbaek Cho
    • , Natividad Ruiz
    • , Thomas G. Bernhardt
    •  & Ry Young
  • Article |

    CD81 is shown to interact with SAMHD1 and lead to its proteasomal degradation, thereby impacting dNTP availability and enhancing HIV-1 reverse transcription in primary human T cells.

    • Vera Rocha-Perugini
    • , Henar Suárez
    • , Susana Álvarez
    • , Soraya López-Martín
    • , Gina M. Lenzi
    • , Felipe Vences-Catalán
    • , Shoshana Levy
    • , Baek Kim
    • , María A. Muñoz-Fernández
    • , Francisco Sánchez-Madrid
    •  & Maria Yáñez-Mó
  • Article |

    TRIM23 is identified as an essential regulator of virus-induced autophagy that mediates restriction to several RNA and DNA viruses. K27-mediated ubiquitylation activates TRIM23 GTPase activity, triggering its relocalization and selective autophagy.

    • Konstantin M. J. Sparrer
    • , Sebastian Gableske
    • , Matthew A. Zurenski
    • , Zachary M. Parker
    • , Florian Full
    • , Gavin J. Baumgart
    • , Jiro Kato
    • , Gustavo Pacheco-Rodriguez
    • , Chengyu Liang
    • , Owen Pornillos
    • , Joel Moss
    • , Martha Vaughan
    •  & Michaela U. Gack
  • Article |

    Chikungunya virus infection leads to painful arthritis-like joint inflammation. This study shows that the NLRP3 inflammasome is crucial for alphavirus-induced inflammation and its inhibition is an effective therapeutic strategy.

    • Weiqiang Chen
    • , Suan-Sin Foo
    • , Ali Zaid
    • , Terk-Shin Teng
    • , Lara J. Herrero
    • , Stefan Wolf
    • , Kothila Tharmarajah
    • , Luan D. Vu
    • , Caryn van Vreden
    • , Adam Taylor
    • , Joseph R. Freitas
    • , Rachel W. Li
    • , Trent M. Woodruff
    • , Richard Gordon
    • , David M. Ojcius
    • , Helder I. Nakaya
    • , Thirumala-Devi Kanneganti
    • , Luke A. J. O’Neill
    • , Avril A. B. Robertson
    • , Nicholas J. King
    • , Andreas Suhrbier
    • , Matthew A. Cooper
    • , Lisa F. P. Ng
    •  & Suresh Mahalingam
  • Letter |

    Toxoplasma gondii uses its proteins RON2, RON4 and RON5 to recruit host proteins, including the ESCRT-I components ALIX and TSG101 to the moving junction, a multimolecular structure that enables invasion.

    • Amandine Guérin
    • , Rosa Milagros Corrales
    • , Michele L. Parker
    • , Mauld H. Lamarque
    • , Damien Jacot
    • , Hiba El Hajj
    • , Dominique Soldati-Favre
    • , Martin J. Boulanger
    •  & Maryse Lebrun
  • Article |

    This study reports the identification of broadly protective antibodies targeting the influenza B neuraminidase away from its active site. One dose of antibody therapy was more protective in mice than multiple doses of the current standard of care.

    • Teddy John Wohlbold
    • , Kira A. Podolsky
    • , Veronika Chromikova
    • , Ericka Kirkpatrick
    • , Veronica Falconieri
    • , Philip Meade
    • , Fatima Amanat
    • , Jessica Tan
    • , Benjamin R. tenOever
    • , Gene S. Tan
    • , Sriram Subramaniam
    • , Peter Palese
    •  & Florian Krammer
  • Article |

    Both African and epidemic strains of Zika virus are shown to target CD14+ monocytes, which are more susceptible in pregnant women, but African strains are associated with inflammatory responses, and epidemic strains with immunotolerance.

    • Suan-Sin Foo
    • , Weiqiang Chen
    • , Yen Chan
    • , James W. Bowman
    • , Lin-Chun Chang
    • , Younho Choi
    • , Ji Seung Yoo
    • , Jianning Ge
    • , Genhong Cheng
    • , Alexandre Bonnin
    • , Karin Nielsen-Saines
    • , Patrícia Brasil
    •  & Jae U. Jung
  • Article |

    A new class of drugs, hexahydroquinolines, inhibits host haemoglobin endocytosis by Plasmodium falciparum and displays both therapeutic and transmission-blocking activities.

    • Manu Vanaerschot
    • , Leonardo Lucantoni
    • , Tao Li
    • , Jill M. Combrinck
    • , Andrea Ruecker
    • , T. R. Santha Kumar
    • , Kelly Rubiano
    • , Pedro E. Ferreira
    • , Giulia Siciliano
    • , Sonia Gulati
    • , Philipp P. Henrich
    • , Caroline L. Ng
    • , James M. Murithi
    • , Victoria C. Corey
    • , Sandra Duffy
    • , Ori J. Lieberman
    • , M. Isabel Veiga
    • , Robert E. Sinden
    • , Pietro Alano
    • , Michael J. Delves
    • , Kim Lee Sim
    • , Elizabeth A. Winzeler
    • , Timothy J. Egan
    • , Stephen L. Hoffman
    • , Vicky M. Avery
    •  & David A. Fidock
  • Letter |

    Metagenomic analyses reveal that microbial genomes undergo a community-wide transition in size and GC content across a narrow depth range, indicating that nutrient limitation is a major driver in marine microbial genomic and proteomic evolution.

    • Daniel R. Mende
    • , Jessica A. Bryant
    • , Frank O. Aylward
    • , John M. Eppley
    • , Torben Nielsen
    • , David M. Karl
    •  & Edward F. DeLong
  • Article |

    During acute HIV type 1 infection, a subset of γδ T cells that express Δ42PD1 are shown to home to the gut, where they activate innate immunity and inflammation through direct interaction of Δ42PD1 with Toll-like receptor 4. Blockade of this pathway reduces mucosal damage.

    • Allen Ka Loon Cheung
    • , Hau-yee Kwok
    • , Yiru Huang
    • , Min Chen
    • , Yufei Mo
    • , Xilin Wu
    • , Ka-shing Lam
    • , Hoi-Kuan Kong
    • , Terrence Chi Kong Lau
    • , Jingying Zhou
    • , Jingjing Li
    • , Lin Cheng
    • , Boon Kiat Lee
    • , Qiaoli Peng
    • , Xiaofan Lu
    • , Minghui An
    • , Hui Wang
    • , Hong Shang
    • , Boping Zhou
    • , Hao Wu
    • , Aimin Xu
    • , Kwok-Yung Yuen
    •  & Zhiwei Chen
  • Letter |

    Isolation of a cyanophage encoding photosystem I genes reveals that these are expressed during infection and inserted into host membranes, resulting in enhanced electron flow, and that phage carrying these genes are abundant in marine environments.

    • Svetlana Fridman
    • , José Flores-Uribe
    • , Shirley Larom
    • , Onit Alalouf
    • , Oded Liran
    • , Iftach Yacoby
    • , Faris Salama
    • , Benjamin Bailleul
    • , Fabrice Rappaport
    • , Tamar Ziv
    • , Itai Sharon
    • , Francisco M. Cornejo-Castillo
    • , Alon Philosof
    • , Christopher L. Dupont
    • , Pablo Sánchez
    • , Silvia G. Acinas
    • , Forest L. Rohwer
    • , Debbie Lindell
    •  & Oded Béjà