Articles

  • Article |

    A mouse mutagenesis screen identifies CYRI (FAM49B) as a host protection factor against Salmonella infection. CYRI negatively regulates RAC1 signalling to interfere with bacterial entry and dissemination.

    • Kyoko E. Yuki
    • , Hadir Marei
    • , Evgenij Fiskin
    • , Megan M. Eva
    • , Angelica A. Gopal
    • , Jeremy A. Schwartzentruber
    • , Jacek Majewski
    • , Mathieu Cellier
    • , Judith N. Mandl
    • , Silvia M. Vidal
    • , Danielle Malo
    •  & Ivan Dikic
  • Article |

    Here, the authors describe the global distribution of crAssphage, its presence in Old-World and New-World primates, and its association with gut bacterial communities and dietary factors, providing insights into the origin, evolution and epidemiology of human gut crAssphage.

    • Robert A. Edwards
    • , Alejandro A. Vega
    • , Holly M. Norman
    • , Maria Ohaeri
    • , Kyle Levi
    • , Elizabeth A. Dinsdale
    • , Ondrej Cinek
    • , Ramy K. Aziz
    • , Katelyn McNair
    • , Jeremy J. Barr
    • , Kyle Bibby
    • , Stan J. J. Brouns
    • , Adrian Cazares
    • , Patrick A. de Jonge
    • , Christelle Desnues
    • , Samuel L. Díaz Muñoz
    • , Peter C. Fineran
    • , Alexander Kurilshikov
    • , Rob Lavigne
    • , Karla Mazankova
    • , David T. McCarthy
    • , Franklin L. Nobrega
    • , Alejandro Reyes Muñoz
    • , German Tapia
    • , Nicole Trefault
    • , Alexander V. Tyakht
    • , Pablo Vinuesa
    • , Jeroen Wagemans
    • , Alexandra Zhernakova
    • , Frank M. Aarestrup
    • , Gunduz Ahmadov
    • , Abeer Alassaf
    • , Josefa Anton
    • , Abigail Asangba
    • , Emma K. Billings
    • , Vito Adrian Cantu
    • , Jane M. Carlton
    • , Daniel Cazares
    • , Gyu-Sung Cho
    • , Tess Condeff
    • , Pilar Cortés
    • , Mike Cranfield
    • , Daniel A. Cuevas
    • , Rodrigo De la Iglesia
    • , Przemyslaw Decewicz
    • , Michael P. Doane
    • , Nathaniel J. Dominy
    • , Lukasz Dziewit
    • , Bashir Mukhtar Elwasila
    • , A. Murat Eren
    • , Charles Franz
    • , Jingyuan Fu
    • , Cristina Garcia-Aljaro
    • , Elodie Ghedin
    • , Kristen M. Gulino
    • , John M. Haggerty
    • , Steven R. Head
    • , Rene S. Hendriksen
    • , Colin Hill
    • , Heikki Hyöty
    • , Elena N. Ilina
    • , Mitchell T. Irwin
    • , Thomas C. Jeffries
    • , Juan Jofre
    • , Randall E. Junge
    • , Scott T. Kelley
    • , Mohammadali Khan Mirzaei
    • , Martin Kowalewski
    • , Deepak Kumaresan
    • , Steven R. Leigh
    • , David Lipson
    • , Eugenia S. Lisitsyna
    • , Montserrat Llagostera
    • , Julia M. Maritz
    • , Linsey C. Marr
    • , Angela McCann
    • , Shahar Molshanski-Mor
    • , Silvia Monteiro
    • , Benjamin Moreira-Grez
    • , Megan Morris
    • , Lawrence Mugisha
    • , Maite Muniesa
    • , Horst Neve
    • , Nam-phuong Nguyen
    • , Olivia D. Nigro
    • , Anders S. Nilsson
    • , Taylor O’Connell
    • , Rasha Odeh
    • , Andrew Oliver
    • , Mariana Piuri
    • , Aaron J. Prussin II
    • , Udi Qimron
    • , Zhe-Xue Quan
    • , Petra Rainetova
    • , Adán Ramírez-Rojas
    • , Raul Raya
    • , Kim Reasor
    • , Gillian A. O. Rice
    • , Alessandro Rossi
    • , Ricardo Santos
    • , John Shimashita
    • , Elyse N. Stachler
    • , Lars C. Stene
    • , Ronan Strain
    • , Rebecca Stumpf
    • , Pedro J. Torres
    • , Alan Twaddle
    • , MaryAnn Ugochi Ibekwe
    • , Nicolás Villagra
    • , Stephen Wandro
    • , Bryan White
    • , Andy Whiteley
    • , Katrine L. Whiteson
    • , Cisca Wijmenga
    • , Maria M. Zambrano
    • , Henrike Zschach
    •  & Bas E. Dutilh
  • Article |

    A combination of structural and biochemical analyses of MlaC, the soluble periplasmic component of the Mla pathway, elucidate how this protein assists phospholipid movement between the bacterial inner and outer membranes.

    • Gareth W. Hughes
    • , Stephen C. L. Hall
    • , Claire S. Laxton
    • , Pooja Sridhar
    • , Amirul H. Mahadi
    • , Caitlin Hatton
    • , Thomas J. Piggot
    • , Peter J. Wotherspoon
    • , Aneika C. Leney
    • , Douglas G. Ward
    • , Mohammed Jamshad
    • , Vaclav Spana
    • , Ian T. Cadby
    • , Christopher Harding
    • , Georgia L. Isom
    • , Jack A. Bryant
    • , Rebecca J. Parr
    • , Yasin Yakub
    • , Mark Jeeves
    • , Damon Huber
    • , Ian R. Henderson
    • , Luke A. Clifton
    • , Andrew L. Lovering
    •  & Timothy J. Knowles
  • Article |

    Collateral sensitivity-inducing mutations in methicillin-resistant Staphylococcus aureus strains lead to re-sensitization of bacteria to penicillin–clavulanic acid combinatorial treatment.

    • Ewan M. Harrison
    • , Xiaoliang Ba
    • , Francesc Coll
    • , Beth Blane
    • , Olivier Restif
    • , Henry Carvell
    • , Claudio U. Köser
    • , Dorota Jamrozy
    • , Sandra Reuter
    • , Andrew Lovering
    • , Nicholas Gleadall
    • , Katherine L. Bellis
    • , Anne-Catrin Uhlemann
    • , Franklin D. Lowy
    • , Ruth C. Massey
    • , Inês R. Grilo
    • , Rita Sobral
    • , Jesper Larsen
    • , Anders Rhod Larsen
    • , Carina Vingsbo Lundberg
    • , Julian Parkhill
    • , Gavin K. Paterson
    • , Matthew T. G. Holden
    • , Sharon J. Peacock
    •  & Mark A. Holmes
  • Article |

    The cryo-electron microscopy structures of apo and promoter-bound influenza D polymerase reveal that the 3ʹ end of the viral RNA can bind at two different sites on the surface of the viral polymerase. Further mutational and biochemical analyses confirm the importance of both sites for viral RNA synthesis.

    • Qi Peng
    • , Yuqian Liu
    • , Ruchao Peng
    • , Min Wang
    • , Wei Yang
    • , Hao Song
    • , Yuhai Chen
    • , Sheng Liu
    • , Min Han
    • , Xinzheng Zhang
    • , Peiyi Wang
    • , Jinghua Yan
    • , Buchang Zhang
    • , Jianxun Qi
    • , Tao Deng
    • , George F. Gao
    •  & Yi Shi
  • Article |

    The use of a statistical prediction model to estimate the determinants of parasite load and host response dynamics in humans infected with the human malaria parasite Plasmodium falciparum identifies cathepsin G and matrix metallopeptidase 9 as host factors that can inhibit parasite invasion into red blood cells.

    • Athina Georgiadou
    • , Hyun Jae Lee
    • , Michael Walther
    • , Anna E. van Beek
    • , Fadlila Fitriani
    • , Diana Wouters
    • , Taco W. Kuijpers
    • , Davis Nwakanma
    • , Umberto D’Alessandro
    • , Eleanor M. Riley
    • , Thomas D. Otto
    • , Azra Ghani
    • , Michael Levin
    • , Lachlan J. Coin
    • , David J. Conway
    • , Michael T. Bretscher
    •  & Aubrey J. Cunnington
  • Article |

    Functional metagenomic screening identified a pair of enzymes from the gut bacterium Flavonifractor plautii that can efficiently convert A antigen to the H antigen of universal O type blood.

    • Peter Rahfeld
    • , Lyann Sim
    • , Haisle Moon
    • , Iren Constantinescu
    • , Connor Morgan-Lang
    • , Steven J. Hallam
    • , Jayachandran N. Kizhakkedathu
    •  & Stephen G. Withers
  • Article |

    Murine norovirus protects intestinal epithelial cells (IECs) from chemical injury by inducing a type I interferon (IFN-I) response in the colon via the viral non-structural protein NS1/2. IFN-I signalling in IECs, in turn, stimulates the production and signalling of the cytoprotective cytokine interleukin-22.

    • Jessica A Neil
    • , Yu Matsuzawa-Ishimoto
    • , Elisabeth Kernbauer-Hölzl
    • , Samantha L Schuster
    • , Stela Sota
    • , Mericien Venzon
    • , Simone Dallari
    • , Antonio Galvao Neto
    • , Ashley Hine
    • , David Hudesman
    • , P’ng Loke
    • , Timothy J Nice
    •  & Ken Cadwell
  • Article | | open

    Statistical mapping techniques provide insights into the current geographical spread of the mosquito-borne dengue virus infection and predict changes in the areas that will be environmentally suitable to the virus for the years 2020, 2050 and 2080.

    • Jane P. Messina
    • , Oliver J. Brady
    • , Nick Golding
    • , Moritz U. G. Kraemer
    • , G. R. William Wint
    • , Sarah E. Ray
    • , David M. Pigott
    • , Freya M. Shearer
    • , Kimberly Johnson
    • , Lucas Earl
    • , Laurie B. Marczak
    • , Shreya Shirude
    • , Nicole Davis Weaver
    • , Marius Gilbert
    • , Raman Velayudhan
    • , Peter Jones
    • , Thomas Jaenisch
    • , Thomas W. Scott
    • , Robert C. Reiner Jr
    •  & Simon I. Hay
  • Article |

    V. cholerae uses its DNA-uptake pili to bind to chitinous surfaces. These filaments also mediate auto-aggregation and, given strain-to-strain variability in PilA (the major pilin subunit), enable discrimination between cells carrying different PilA subunits.

    • David. W. Adams
    • , Sandrine Stutzmann
    • , Candice Stoudmann
    •  & Melanie Blokesch
  • Article |

    The sialic acid-binding Ig-like lectin 1 (Siglec-1, also known as CD169) plays a more prominent role than the C-type lectin dendritic cell-specific intercellular adhesion molecule-3-Grabbing non-integrin in mediating Ebola virus entry into activated dendritic cells, and anti-Siglec-1 monoclonal antibodies can antagonize this process.

    • Daniel Perez-Zsolt
    • , Itziar Erkizia
    • , Maria Pino
    • , Mónica García-Gallo
    • , Maria Teresa Martin
    • , Susana Benet
    • , Jakub Chojnacki
    • , María Teresa Fernández-Figueras
    • , Dolores Guerrero
    • , Victor Urrea
    • , Xabier Muñiz-Trabudua
    • , Leonor Kremer
    • , Javier Martinez-Picado
    •  & Nuria Izquierdo-Useros
  • Article |

    The gut microbiota member Bacteroides thetaiotaomicron can utilize various complex N-glycans, including those from immunoglobulins, via a complex and diverse set of enzymes encoded by multiple non-adjacent loci.

    • Justina Briliūtė
    • , Paulina A. Urbanowicz
    • , Ana S. Luis
    • , Arnaud Baslé
    • , Neil Paterson
    • , Osmond Rebello
    • , Jenifer Hendel
    • , Didier A. Ndeh
    • , Elisabeth C. Lowe
    • , Eric C. Martens
    • , Daniel I. R. Spencer
    • , David N. Bolam
    •  & Lucy I. Crouch
  • Article |

    Systematic deletion analysis of Fusarium graminearum G-protein-coupled receptor genes leads to the identification of five genes that are significantly upregulated during wheat infection and required for virulence.

    • Cong Jiang
    • , Shulin Cao
    • , Zeyi Wang
    • , Huaijian Xu
    • , Jie Liang
    • , Huiquan Liu
    • , Guanghui Wang
    • , Mingyu Ding
    • , Qinhu Wang
    • , Chen Gong
    • , Chanjing Feng
    • , Chaofeng Hao
    •  & Jin-Rong Xu
  • Article |

    The interferon-inducible RNA-binding protein N4BP1 binds to and degrades HIV-1 mRNAs, thus inhibiting viral degradation and promoting viral latency. However, metacaspase-mediated degradation of N4BP1 following activation of CD4+ T cells facilitates HIV-1 latency reversal.

    • Daichi Yamasoba
    • , Kei Sato
    • , Takuya Ichinose
    • , Tomoko Imamura
    • , Lennart Koepke
    • , Simone Joas
    • , Elisabeth Reith
    • , Dominik Hotter
    • , Naoko Misawa
    • , Kotaro Akaki
    • , Takuya Uehata
    • , Takashi Mino
    • , Sho Miyamoto
    • , Takeshi Noda
    • , Akio Yamashita
    • , Daron M. Standley
    • , Frank Kirchhoff
    • , Daniel Sauter
    • , Yoshio Koyanagi
    •  & Osamu Takeuchi
  • Article |

    The structures of two neutralizing human monoclonal antibodies against Plasmodium vivax Duffy-binding protein (DBP) isolated from an individual with naturally acquired immunity, bound to DBP, reveal how such antibodies prevent parasite entry into reticulocytes.

    • Darya Urusova
    • , Lenore Carias
    • , Yining Huang
    • , Vanessa C. Nicolete
    • , Jean Popovici
    • , Camille Roesch
    • , Nichole D. Salinas
    • , Benoit Witkowski
    • , Marcelo U. Ferreira
    • , John H. Adams
    • , Michael L. Gross
    • , Christopher L. King
    •  & Niraj H. Tolia
  • Article |

    The structure of a neutralizing human monoclonal antibody isolated from an individual immunized in a Plasmodium vivax Duffy binding protein vaccine clinical trial, in complex with its target, elucidates the mechanism by which parasite invasion of immature red blood cells is inhibited.

    • Thomas. A. Rawlinson
    • , Natalie M. Barber
    • , Franziska Mohring
    • , Jee Sun Cho
    • , Varakorn Kosaisavee
    • , Samuel F. Gérard
    • , Daniel G. W. Alanine
    • , Geneviève M. Labbé
    • , Sean C. Elias
    • , Sarah E. Silk
    • , Doris Quinkert
    • , Jing Jin
    • , Jennifer M. Marshall
    • , Ruth O. Payne
    • , Angela M. Minassian
    • , Bruce Russell
    • , Laurent Rénia
    • , François H. Nosten
    • , Robert W. Moon
    • , Matthew K. Higgins
    •  & Simon J. Draper
  • Article |

    The electron cryo-microscopy structure of Sulfolobus islandicus pili enabled the identification of SiL_2606 as the main pilin in these filaments and revealed that the pili are glycosylated, which probably explains how these structures remain soluble and stable even when cells grow at pH 3 and 80 °C.

    • Fengbin Wang
    • , Virginija Cvirkaite-Krupovic
    • , Mark A. B. Kreutzberger
    • , Zhangli Su
    • , Guilherme A. P. de Oliveira
    • , Tomasz Osinski
    • , Nicholas Sherman
    • , Frank DiMaio
    • , Joseph S. Wall
    • , David Prangishvili
    • , Mart Krupovic
    •  & Edward H. Egelman
  • Article |

    VacA promotes Helicobacter pylori intracellular localization and persistence in the face of antibiotics. Activation of the lysosomal TRPML1 calcium channel reverses VacA toxicity and promotes bacterial clearance during drug treatment.

    • Mariana I. Capurro
    • , Laura K. Greenfield
    • , Akriti Prashar
    • , Sunny Xia
    • , Majd Abdullah
    • , Harikesh Wong
    • , Xi Zoe Zhong
    • , Nina Bertaux-Skeirik
    • , Jayati Chakrabarti
    • , Iram Siddiqui
    • , Catherine O’Brien
    • , Xianping Dong
    • , Lisa Robinson
    • , Richard M. Peek Jr
    • , Dana J. Philpott
    • , Yana Zavros
    • , Michael Helmrath
    •  & Nicola L. Jones
  • Article |

    Leukotriene B4 signalling contributes to disease tolerance to influenza virus infection by stimulating the production of interferon-α (IFN-α) by interstitial macrophages. IFN-α, in turn, suppresses in situ inflammatory, monocyte-derived macrophage proliferation and mitigates lung immunopathology.

    • Erwan Pernet
    • , Jeffrey Downey
    • , Donald C. Vinh
    • , William S. Powell
    •  & Maziar Divangahi
  • Article | | open

    Using metagenomics and proteomics on Mediterranean grassland soil samples resulted in the recovery of 793 metagenome-assembled genomes and provided insights into microbial activity in this environment, and how this changes with soil depth and exposure to extended rainfall.

    • Spencer Diamond
    • , Peter F. Andeer
    • , Zhou Li
    • , Alexander Crits-Christoph
    • , David Burstein
    • , Karthik Anantharaman
    • , Katherine R. Lane
    • , Brian C. Thomas
    • , Chongle Pan
    • , Trent R. Northen
    •  & Jillian F. Banfield
  • Article |

    The GTPase MglA, its cognate GTPase activating protein (GAP) MglB and RomR are known to regulate gliding motility in Myxococcus xanthus. There are no known cognate guanine nucleotide exchange factors (GEFs) for MglA. Here, RomX is identified as a binding partner of RomR, and their complex serves as an MglA GEF.

    • Dobromir Szadkowski
    • , Andrea Harms
    • , Luis António Menezes Carreira
    • , Manon Wigbers
    • , Anna Potapova
    • , Kristin Wuichet
    • , Daniela Keilberg
    • , Ulrich Gerland
    •  & Lotte Søgaard-Andersen
  • Article |

    Direct interaction of influenza virus with Gram-positive and Gram-negative bacterial pathogens of the respiratory tract results in increased bacterial adherence to respiratory epithelial cells in culture and in higher bacterial burdens in vivo.

    • Hannah M. Rowe
    • , Victoria A. Meliopoulos
    • , Amy Iverson
    • , Perrine Bomme
    • , Stacey Schultz-Cherry
    •  & Jason W. Rosch
  • Article |

    Mycobacterium tuberculosis secreted protein MPT53 mediates the activation of TAK1 and induces the host immune inflammatory response.

    • Lin Wang
    • , Zhonghua Liu
    • , Jie Wang
    • , Haipeng Liu
    • , Juehui Wu
    • , Tianqi Tang
    • , Haohao Li
    • , Hua Yang
    • , Lianhua Qin
    • , Dapeng Ma
    • , Jianxia Chen
    • , Feng Liu
    • , Peng Wang
    • , Ruijuan Zheng
    • , Peng Song
    • , Yilong Zhou
    • , Zhenling Cui
    • , Xiangyang Wu
    • , Xiaochen Huang
    • , Haijiao Liang
    • , Shanshan Zhang
    • , Jingjing Cao
    • , Chunyan Wu
    • , Yiping Chen
    • , Dan Su
    • , Xinchun Chen
    • , Gucheng Zeng
    •  & Baoxue Ge
  • Article |

    Co-administration of a whole-inactivated influenza virus (IAV) vaccine (γ-Flu) with a whole-inactivated Streptococcus pneumoniae vaccine (γ-PN) enhances IAV-specific immune responses due to the ability of γ-PN to directly interact with γ-Flu, thus increasing viral uptake by macrophages.

    • Shannon C. David
    • , Todd Norton
    • , Timona Tyllis
    • , Jasmine J. Wilson
    • , Eve V. Singleton
    • , Zoe Laan
    • , Justin Davies
    • , Timothy R. Hirst
    • , Iain Comerford
    • , Shaun R. McColl
    • , James C. Paton
    •  & Mohammed Alsharifi
  • Article |

    Phylogenetic analysis based on a concatenated set of 77 single-copy marker genes enabled the classification of tailed double-stranded DNA viruses from large datasets, and was reproducible across software and models, providing a framework that could be applied to other viruses.

    • Soo Jen Low
    • , Mária Džunková
    • , Pierre-Alain Chaumeil
    • , Donovan H. Parks
    •  & Philip Hugenholtz
  • Article |

    SEDS family peptidoglycan transglycosylases, RodA and FtsW, in Staphylococcus aureus pair with the cognate transpeptidases PBP3 and PBP1 to mediate sidewall elongation and septal peptidoglycan incorporation, respectively, and maintain coccoid morphology.

    • Nathalie T. Reichmann
    • , Andreia C. Tavares
    • , Bruno M. Saraiva
    • , Ambre Jousselin
    • , Patricia Reed
    • , Ana R. Pereira
    • , João M. Monteiro
    • , Rita G. Sobral
    • , Michael S. VanNieuwenhze
    • , Fábio Fernandes
    •  & Mariana G. Pinho
  • Article |

    Global sampling of microbial communities associated with wastewater treatment plants and application of ecological theory revealed a small, core bacterial community associated with performance and provides insights into the community dynamics in this environment.

    • Linwei Wu
    • , Daliang Ning
    • , Bing Zhang
    • , Yong Li
    • , Ping Zhang
    • , Xiaoyu Shan
    • , Qiuting Zhang
    • , Mathew Brown
    • , Zhenxin Li
    • , Joy D. Van Nostrand
    • , Fangqiong Ling
    • , Naijia Xiao
    • , Ya Zhang
    • , Julia Vierheilig
    • , George F. Wells
    • , Yunfeng Yang
    • , Ye Deng
    • , Qichao Tu
    • , Aijie Wang
    • , Dany Acevedo
    • , Miriam Agullo-Barcelo
    • , Pedro J. J. Alvarez
    • , Lisa Alvarez-Cohen
    • , Gary L. Andersen
    • , Juliana Calabria de Araujo
    • , Kevin Boehnke
    • , Philip Bond
    • , Charles B. Bott
    • , Patricia Bovio
    • , Rebecca K. Brewster
    • , Faizal Bux
    • , Angela Cabezas
    • , Léa Cabrol
    • , Si Chen
    • , Craig S. Criddle
    • , Ye Deng
    • , Claudia Etchebehere
    • , Amanda Ford
    • , Dominic Frigon
    • , Janeth Sanabria Gómez
    • , James S. Griffin
    • , April Z. Gu
    • , Moshe Habagil
    • , Lauren Hale
    • , Steven D. Hardeman
    • , Marc Harmon
    • , Harald Horn
    • , Zhiqiang Hu
    • , Shameem Jauffur
    • , David R. Johnson
    • , Jurg Keller
    • , Alexander Keucken
    • , Sheena Kumari
    • , Cintia Dutra Leal
    • , Laura A. Lebrun
    • , Jangho Lee
    • , Minjoo Lee
    • , Zarraz M. P. Lee
    • , Yong Li
    • , Zhenxin Li
    • , Mengyan Li
    • , Xu Li
    • , Fangqiong Ling
    • , Yu Liu
    • , Richard G. Luthy
    • , Leda C. Mendonça-Hagler
    • , Francisca Gleire Rodriguez de Menezes
    • , Arthur J. Meyers
    • , Amin Mohebbi
    • , Per H. Nielsen
    • , Daliang Ning
    • , Adrian Oehmen
    • , Andrew Palmer
    • , Prathap Parameswaran
    • , Joonhong Park
    • , Deborah Patsch
    • , Valeria Reginatto
    • , Francis L. de los Reyes III
    • , Bruce E. Rittmann
    • , Adalberto Noyola Robles
    • , Simona Rossetti
    • , Xiaoyu Shan
    • , Jatinder Sidhu
    • , William T. Sloan
    • , Kylie Smith
    • , Oscarina Viana de Sousa
    • , David A. Stahl
    • , Kyle Stephens
    • , Renmao Tian
    • , James M. Tiedje
    • , Nicholas B. Tooker
    • , Qichao Tu
    • , Joy D. Van Nostrand
    • , Daniel De los Cobos Vasconcelos
    • , Julia Vierheilig
    • , Michael Wagner
    • , Steve Wakelin
    • , Aijie Wang
    • , Bei Wang
    • , Joseph E. Weaver
    • , George F. Wells
    • , Stephanie West
    • , Paul Wilmes
    • , Sung-Geun Woo
    • , Linwei Wu
    • , Jer-Horng Wu
    • , Liyou Wu
    • , Chuanwu Xi
    • , Naijia Xiao
    • , Meiying Xu
    • , Tao Yan
    • , Yunfeng Yang
    • , Min Yang
    • , Michelle Young
    • , Haowei Yue
    • , Bing Zhang
    • , Ping Zhang
    • , Qiuting Zhang
    • , Ya Zhang
    • , Tong Zhang
    • , Qian Zhang
    • , Wen Zhang
    • , Yu Zhang
    • , Hongde Zhou
    • , Jizhong Zhou
    • , Xianghua Wen
    • , Thomas P. Curtis
    • , Qiang He
    • , Zhili He
    • , Matthew Brown
    • , Tong Zhang
    • , Zhili He
    • , Jurg Keller
    • , Per H. Nielsen
    • , Pedro J. J. Alvarez
    • , Craig S. Criddle
    • , Michael Wagner
    • , James M. Tiedje
    • , Qiang He
    • , Thomas P. Curtis
    • , David A. Stahl
    • , Lisa Alvarez-Cohen
    • , Bruce E. Rittmann
    • , Xianghua Wen
    •  & Jizhong Zhou
  • Article |

    Following export from the parasite, the dense granule-resident effector TEEGR travels to the host cell nucleus where it interferes with host transcription factors to modulate immune signalling and regulate Toxoplasma persistence.

    • Laurence Braun
    • , Marie-Pierre Brenier-Pinchart
    • , Pierre-Mehdi Hammoudi
    • , Dominique Cannella
    • , Sylvie Kieffer-Jaquinod
    • , Julien Vollaire
    • , Véronique Josserand
    • , Bastien Touquet
    • , Yohann Couté
    • , Isabelle Tardieux
    • , Alexandre Bougdour
    •  & Mohamed-Ali Hakimi
  • Article |

    A combination of electron cryotomography and immunofluorescence microscopy reveals the structure of the core transmembrane subcomplex of the Legionella defective in organelle trafficking (Dot)/intracellular multiplication (Icm) type IVB secretion system (T4BSS) and an early-stage assembly process by which T4BSS components are targeted to the bacterial poles by DotU and IcmF.

    • Debnath Ghosal
    • , Kwangcheol C. Jeong
    • , Yi-Wei Chang
    • , Jacob Gyore
    • , Lin Teng
    • , Adam Gardner
    • , Joseph P. Vogel
    •  & Grant J. Jensen
  • Article |

    The ADP-ribosyltransferase PARP11 promotes ubiquitination and degradation of the interferon type I (IFN-I) receptor IFNAR1 by mono-ADP-ribosylating the ubiquitin E3 ligase β-TrCP, thus suppressing the IFN-I-mediated antiviral responses both in vitro and in vivo.

    • Tingting Guo
    • , Yibo Zuo
    • , Liping Qian
    • , Jin Liu
    • , Yukang Yuan
    • , Kailin Xu
    • , Ying Miao
    • , Qian Feng
    • , Xiangjie Chen
    • , Lincong Jin
    • , Liting Zhang
    • , Chunsheng Dong
    • , Sidong Xiong
    •  & Hui Zheng
  • Article |

    A nonlinear time-series analysis was used to identify antibiotic use thresholds that are associated with the emergence of antibiotic resistance.

    • José-María López-Lozano
    • , Timothy Lawes
    • , César Nebot
    • , Arielle Beyaert
    • , Xavier Bertrand
    • , Didier Hocquet
    • , Mamoon Aldeyab
    • , Michael Scott
    • , Geraldine Conlon-Bingham
    • , David Farren
    • , Gábor Kardos
    • , Adina Fésűs
    • , Jesús Rodríguez-Baño
    • , Pilar Retamar
    • , Nieves Gonzalo-Jiménez
    • , Ian M. Gould
    • , José-María López-Lozano
    • , Timothy Lawes
    • , César Nebot
    • , Arielle Beyaert
    • , Xavier Bertrand
    • , Didier Hocquet
    • , Mamoon Aldeyab
    • , Michael Scott
    • , Geraldine Conlon-Bingham
    • , David Farren
    • , Gábor Kardos
    • , Adina Fésus
    • , Jesús Rodríguez-Baño
    • , Pilar Retamar
    • , Nieves Gonzalo-Jiménez
    • , Ian M. Gould
    • , María Núñez-Núñez
    • , Ana I. Suárez
    • , Michelle Thouverez
    • , María Navarro-Cots
    • , Emilio Borrajo
    • , Carlos Devesa
    • , Joan Gregori
    • , Inmaculada García-Cuello
    • , Isabel Pacheco
    • , María Cerón
    •  & Hajnalka Tóth
  • Article |

    Human DNase I is shown to be overexpressed in hypoxic environments in vitro and to be incorporated into human hepatitis B virus (HBV) particles, resulting in virions devoid of genomic DNA. These findings might help explain the low levels of circulating HBV DNA commonly observed in cirrhotic livers and liver tumours.

    • Camille Hallez
    • , Xiongxiong Li
    • , Rodolphe Suspène
    • , Valérie Thiers
    • , Mohamed S Bouzidi
    • , Cristina M Dorobantu
    • , Vincent Lucansky
    • , Simon Wain-Hobson
    • , Raphaël Gaudin
    •  & Jean-Pierre Vartanian
  • Article |

    The study of the impact of mutations in the glutamate dehydrogenase enzyme from Bacillus subtilis across multiple settings, including liquid cultures, biofilms and in soil, highlights how bacterial growth states and the environment can differentially impact protein evolution.

    • Lianet Noda-García
    • , Dan Davidi
    • , Elisa Korenblum
    • , Assaf Elazar
    • , Ekaterina Putintseva
    • , Asaph Aharoni
    •  & Dan S. Tawfik
  • Article |

    The isolation and characterization of glycoprotein-specific monoclonal antibodies from a patient with Rift Valley fever (RVF) provide insights into the humoral immune responses elicited by the RVF virus as well as the therapeutic potential of antibody-based strategies.

    • Qihui Wang
    • , Tong Ma
    • , Yan Wu
    • , Zhihai Chen
    • , Hui Zeng
    • , Zhou Tong
    • , Feng Gao
    • , Jianxun Qi
    • , Zhennan Zhao
    • , Yan Chai
    • , Huabing Yang
    • , Gary Wong
    • , Yuhai Bi
    • , Lili Wu
    • , Rui Shi
    • , Mi Yang
    • , Jian Song
    • , Haihai Jiang
    • , Zhiqiang An
    • , Junzhi Wang
    • , Tilahun D. Yilma
    • , Yi Shi
    • , William J. Liu
    • , Mifang Liang
    • , Chuan Qin
    • , George F. Gao
    •  & Jinghua Yan
  • Article |

    Here, the authors characterize the symbiotic relationship between Haliclona sponges and its intracellular symbiont. The symbiont has a highly streamlined genome, yet retains a plasmid with the biosynthetic gene cluster for production of the defence chemical renieramycin and lives within chemobacteriocytes for nutrient acquisition.

    • Ma. Diarey Tianero
    • , Jared N. Balaich
    •  & Mohamed S. Donia
  • Article |

    Variable susceptibility to Salmonella infection across genetically similar mice from commercial vendors is due to differential colonization of the gut microbiome by endogenous Enterobacteriaceae.

    • Eric M. Velazquez
    • , Henry Nguyen
    • , Keaton T. Heasley
    • , Cheng H. Saechao
    • , Lindsey M. Gil
    • , Andrew W. L. Rogers
    • , Brittany M. Miller
    • , Matthew R. Rolston
    • , Christopher A. Lopez
    • , Yael Litvak
    • , Megan J. Liou
    • , Franziska Faber
    • , Denise N. Bronner
    • , Connor R. Tiffany
    • , Mariana X. Byndloss
    • , Austin J. Byndloss
    •  & Andreas J. Bäumler
  • Article |

    Phenothiazines inhibit Type IV pili function in Neisseria meningitidis and protect against bacterial colonization of blood vessels and lethality during infection.

    • Kevin Denis
    • , Marion Le Bris
    • , Loic Le Guennec
    • , Jean-Philippe Barnier
    • , Camille Faure
    • , Anne Gouge
    • , Haniaa Bouzinba-Ségard
    • , Anne Jamet
    • , Daniel Euphrasie
    • , Beatrice Durel
    • , Nicolas Barois
    • , Philippe Pelissier
    • , Philippe C. Morand
    • , Mathieu Coureuil
    • , Frank Lafont
    • , Olivier Join-Lambert
    • , Xavier Nassif
    •  & Sandrine Bourdoulous
  • Article |

    By combining a deep mutational scanning strategy with molecular modelling, electron microscopy as well as cellular and biochemical approaches, the authors identify key amino acids in the C-terminal domain of the Zika virus envelope protein (E) that regulate viral replication in mosquito and human cells.

    • Yin Xiang Setoh
    • , Alberto A. Amarilla
    • , Nias Y. G. Peng
    • , Rebecca E. Griffiths
    • , Julio Carrera
    • , Morgan E. Freney
    • , Eri Nakayama
    • , Shinya Ogawa
    • , Daniel Watterson
    • , Naphak Modhiran
    • , Faith Elizabeth Nanyonga
    • , Francisco J. Torres
    • , Andrii Slonchak
    • , Parthiban Periasamy
    • , Natalie A. Prow
    • , Bing Tang
    • , Jessica Harrison
    • , Jody Hobson-Peters
    • , Thom Cuddihy
    • , Justin Cooper-White
    • , Roy A. Hall
    • , Paul R. Young
    • , Jason M. Mackenzie
    • , Ernst Wolvetang
    • , Jesse D. Bloom
    • , Andreas Suhrbier
    •  & Alexander A. Khromykh
  • Article |

    The isolation and characterization of seven monoclonal antibodies that recognize the lateral surface of the influenza virus neuraminidase (NA) head reveal that, even in absence of NA-inhibiting activity, anti-NA antibodies can provide protection and drive antigenic drift at the lateral surface of the NA.

    • Atsuhiro Yasuhara
    • , Seiya Yamayoshi
    • , Maki Kiso
    • , Yuko Sakai-Tagawa
    • , Michiko Koga
    • , Eisuke Adachi
    • , Tadashi Kikuchi
    • , I-Hsuan Wang
    • , Shinya Yamada
    •  & Yoshihiro Kawaoka
  • Article |

    Here, the authors developed an automated, high-throughput method for the sorting of single live cells based on their functional phenotype for downstream genomics or cultivation. Application of the platform to murine gut microbiota samples identified a diverse community of mucin degraders.

    • Kang Soo Lee
    • , Márton Palatinszky
    • , Fátima C. Pereira
    • , Jen Nguyen
    • , Vicente I. Fernandez
    • , Anna J. Mueller
    • , Filippo Menolascina
    • , Holger Daims
    • , David Berry
    • , Michael Wagner
    •  & Roman Stocker
  • Article |

    NOD-like receptor family pyrin domain-containing 6 (NLRP6) reduces colitis severity in a gut microbiome-dependent manner; however, in the context of murine intestinal graft-versus-host disease, NLRP6 exacerbates symptoms independently of gut microbiome composition and diversity, with NLRP6-deficient animals displaying protection against disease.

    • Tomomi Toubai
    • , Hideaki Fujiwara
    • , Corinne Rossi
    • , Mary Riwes
    • , Hiroya Tamaki
    • , Cynthia Zajac
    • , Chen Liu
    • , Anna V. Mathew
    • , Jaeman Byun
    • , Katherine Oravecz-Wilson
    • , Ikuo Matsuda
    • , Yaping Sun
    • , Daniel Peltier
    • , Julia Wu
    • , Jiachen Chen
    • , Sergey Seregin
    • , Israel Henig
    • , Stephanie Kim
    • , Stuart Brabbs
    • , Subramaniam Pennathur
    • , Grace Chen
    •  & Pavan Reddy
  • Article |

    Using integrated metagenomics, metaproteomics and metabolomics approaches, the authors characterize the microbial taxa and metabolic pathways facilitating lignocellulose degradation across gut compartments of a wood-feeding beetle.

    • Javier A. Ceja-Navarro
    • , Ulas Karaoz
    • , Markus Bill
    • , Zhao Hao
    • , Richard A. White III
    • , Abelardo Arellano
    • , Leila Ramanculova
    • , Timothy R. Filley
    • , Timothy D. Berry
    • , Mark E. Conrad
    • , Meredith Blackwell
    • , Carrie D. Nicora
    • , Young-Mo Kim
    • , Patrick N. Reardon
    • , Mary S. Lipton
    • , Joshua N. Adkins
    • , Jennifer Pett-Ridge
    •  & Eoin L. Brodie