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Scanning electron micrograph of a human mesenchymal stem cell grown on an array of posts 12.9 micrometers high. Cover by Erin Dewalt, based on an image provided by Christopher Chen. Brief Communication p733
A Human Proteome Project has been proposed. To ensure that such a project will have a transformative impact on biology, its scope should be determined by wide and open discussion.
Application of single-molecule methods to assay genome-wide mRNA and protein levels in single bacteria provides a systems-level view of their relationship.
The strength of selection that acts on a growing population of cells and the presence of a stochastic switching mechanism may be inferred from lineage data.
Mass spectrometry has evolved and matured to a level where it is able to assess the complexity of the human proteome. We discuss some of the expected challenges ahead and promising strategies for success.
This resource provides a comprehensive evaluation of rodent locomotor profiles after different types of lesions to the central nervous system. The data set can guide the selection of suitable lesion paradigms, locomotor tasks and readouts in future animal studies.
The authors compare quality metrics of libraries from seven strand-specific RNA-seq methods in terms of complexity, strand specificity, evenness and continuity of coverage, and expression profiling. They provide a computational pipeline to compare these metrics from any RNA-seq protocol.
Use of a trimethoprim chemical tag allows super-resolution live-cell microscopy by stochastic single molecule–based localization imaging of the dynamics of genetically tagged histone H2B in cell nuclei.
A gene conferring neomycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using puromycin.
A gene conferring puromycin resistance can be used for antibiotic selection in C. elegans and C. briggsae. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using neomycin.
Engineering of the Ca2+-sensing domain in existing yellow Cameleon Ca2+ indicators is used to create indicators with a range of increased Ca2+ affinities capable of detecting subtle changes in intracellular Ca2+ at low resting levels.
Micropost arrays can be used to modulate substrate rigidity independently of other substrate properties, permitting the study of the effects of rigidity on cell function.
A simple automated system to simultaneously measure growth rate and lag time of large numbers of bacteria permits the identification of subsets in a heterogenous population.
The combination of protein display, moderate selection for protein activity and high-throughput DNA sequencing can be applied to hundreds of thousands of protein variants in parallel, enabling the derivation of sequence-function relationships.
Incorporation of time information into the annotation of distinct biological states in automated fluorescence time-lapse live-cell imaging of complex cellular dynamics reduces both classification noise and confusion between cell states with similar morphology. A computational framework for achieving this is implemented in the open-source software package CellCognition.
Two-photon excitation of a phosphorescent nanoprobe that is quenched by molecular oxygen permits high-resolution measurements of oxygen in both the vasculature and tissue of rodent brain.
A computational approach to both measure and infer microtubule dynamics from time-lapse images of end-labeled microtubules is described. It permits intracellular spatial patterns in microtubule behavior to be monitored.