No. 5 May 2023Temporal profiling of the secretome
Capture of secreted proteins onto their source cell surfaces using an affinity matrix enables simultaneous measurement of protein secretion, cell surface proteins and transcriptomes in thousands of cells at single-cell resolution.
See Wu et al.
No. 4 April 2023Miniscopes for imaging in mice and juvenile zebra finches
Miniature head-mounted microscopes provide expansive views of neurons in songbird and mouse brains.
See Scherrer et al.
No. 3 March 2023Focus on single-cell proteomics
Multiple established and emerging technologies are being applied for the analysis of the single-cell proteome, as covered in this month’s Focus on single-cell proteomics.
No. 2 February 2023Robust signal separation in multiplexed fluorescence microscopy
Highly multiplexed fluorescence images of embryonic zebrafish using data analyzed with the Hybrid Unmixing (HyU) method.
See Chiang et al.
No. 1 January 2023Method of the Year 2022: Long-read sequencing
Long-read sequencing is our pick for the Method of the Year 2022, owing to its unparalleled utility in reading genomes, transcriptomes and epigenomes with high accuracy and completeness.