Method of the Year 2023: methods for modeling development

Methods for modeling development are our pick for the Method of the Year 2023. The cover shows mouse blastocysts stained for trophectoderm (cyan), epiblast (yellow) and primitive endoderm (magenta).

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Neural manipulation with hydrogel-based optical fibers

Soft hydrogel fibers deliver light to peripheral nerve parts to inhibit pain.

See Liu et al.

Robust denoising of voltage imaging data

An artistic illustration of a neural network being used to denoise voltage imaging data.

See Eom et al.

Voltage-imaging-guided single-cell transcriptomics

All-optical voltage imaging with Voltron-expressing neurons (orange cells) optogenetically stimulated with blue light. The neuronal signals are represented by the yellow lines.

See Csillag et al.

Exploring circular RNA

A game of ring toss represents a benchmarking study that assessed 16 bioinformatics tools (stakes) for their abilities to capture true-positive and false-positive circular RNAs (complete and incomplete rings, respectively).

See Vromman et al.

The future of bioimage analysis

A composite image of developing zebrafish embryos imaged with light sheet microscopy and restored with Real-ESRGAN for artistic purposes.

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Native mass spectrometry reveals membrane protein–lipid complexes

A native mass spectrometry platform captures membrane protein–lipid organization directly from tunable lipid membranes and affords molecular understanding of how specific membrane lipids and biophysical properties regulate these assemblies.

See Panda et al.

Temporal profiling of the secretome

Capture of secreted proteins onto their source cell surfaces using an affinity matrix enables simultaneous measurement of protein secretion, cell surface proteins and transcriptomes in thousands of cells at single-cell resolution.

See Wu et al.

Miniscopes for imaging in mice and juvenile zebra finches

Miniature head-mounted microscopes provide expansive views of neurons in songbird and mouse brains.

See Scherrer et al.

Focus on single-cell proteomics

Multiple established and emerging technologies are being applied for the analysis of the single-cell proteome, as covered in this month’s Focus on single-cell proteomics.

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Robust signal separation in multiplexed fluorescence microscopy

Highly multiplexed fluorescence images of embryonic zebrafish using data analyzed with the Hybrid Unmixing (HyU) method.

See Chiang et al.

Method of the Year 2022: Long-read sequencing

Long-read sequencing is our pick for the Method of the Year 2022, owing to its unparalleled utility in reading genomes, transcriptomes and epigenomes with high accuracy and completeness.

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