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A human cell in mitosis observed using cryo-expansion microscopy (Cryo-ExM). The DNA is stained pink and the rest of the cell with an NHS ester that marks the proteome and highlights the mitochondria in black at each pole of the mitotic spindle.
Nature Methods is introducing a new article format: Registered Reports. We encourage all authors interested in submitting comparative analyses of the performance of established, related tools or methods to familiarize themselves with this alternative approach to peer review.
Cryofixation-based ultrastructure-expansion microscopy (cryo-ExM) bypasses artifacts caused by chemical fixation and establishes more-native preservation of biological samples.
This Perspective offers an overview of organic dyes commonly used as fluorescent labels and gives a chemist’s insight into their benefits and peculiarities.
An engineered bacterial nitroreductase, NTR 2.0, improves chemically induced cell ablation, facilitating novel sustained ablation paradigms for testing the effects of chronic inflammation on regeneration, and modeling degenerative disease.
Cryo-ExM combines the expansion microscopy for super-resolution imaging with cryofixation for ultrastructure preservation. Cryo-ExM outperforms established fixation methods on a range of sensitive subcellular structures.
Iso-imaging integrates stable-isotope infusions with imaging mass spectrometry to enable quantitative analysis of metabolic activity in mammalian tissues with spatial resolution. IsoScope software facilitates analysis of iso-imaging data.
OxLight1 is a genetically encoded sensor for the orexin neuropeptides. It has been applied in fiber photometry recordings and two-photon imaging in mice during a variety of behaviors.
VascuViz represents a versatile workflow for multimodal imaging of the vasculature in ex vivo tissue samples across length and resolution scales, paving the way for improved and novel image-based vascular systems biology applications.