Volume 18 Issue 9, September 2021

She connects equity, samurai swords, imaging and an automated way to count and track mitochondria.

Researchers use an in vitro assay in combination with a polymer model to study protein–DNA condensation and how condensates bring distant DNA elements into close proximity.

A co-culture system to mimic the development of functional mouse oocytes in vitro.

A software platform for analyzing data-independent acquisition (DIA) proteomics data.

A single-molecule calorimeter measures the thermodynamic properties of individual proteins.

Sensitive synthetic protein-phosphorylation networks manifest fast bistable dynamics.

Proteins and sugars interact in sweet, complex ways. More tools are emerging, but working in this field still takes endurance.

This Review describes spatial omics and multiplexed imaging technologies and their current and future impact in studying tumor heterogeneity and cancer biology.

This Review describes the state of the art in imaging extracellular vesicles in animals to study their release, biodistribution and uptake, and covers labeling strategies, microscopy methods and discoveries made in model organisms.

This Review discusses optogenetic tools for manipulating endogenous targets such as genes and signaling pathways in a physiological range.

The LIVECell dataset comprises annotated phase-contrast images of over 1.6 million cells from different cell lines during growth from sparse seeding to confluence for improved training of deep learning-based models of image segmentation.

This analysis systematically evaluates cross-linking chemistry and chromatin fragmentation strategies commonly used in 3C assays and introduces an improved Hi-C protocol for detecting loops and compartments.

SnapHiC offers a computational tool for improving detection of chromatin loops from single-cell Hi-C data.

This work describes m6A-seq2, which utilizes multiplexed N⁶-methyladenosine (m6A) immunoprecipitation of pre-barcoded and pooled samples to reduce the technical variability and allows direct comparison of m6A at the site, gene and sample level.

This work describes nascent Ribo-Seq, which measures the speed of ribosome loading and polysome assembly on endogenous mRNA through a combination of 4-thiouridine labeling and ribosome footprint sequencing.

Long-range CRISPR activation can be enhanced by concurrent recruitment of artificial TFs to the enhancer and promoter of a target gene. This CRISPR activation system can be employed to achieve allele-selective gene upregulation by differentially targeting single-nucleotide polymorphisms embedded in enhancers or other distally located sequences.

DECODE uses deep learning for localizing single emitters in high-density two-dimensional and three-dimensional single-molecule localization microscopy data. DECODE outperforms available methods and enables fast live-cell SMLM of dynamic processes.

Mitometer enables efficient, rapid, and accurate automated segmentation and tracking of mitochondria from time-lapse images. Mitometer performs well on diverse input images and can be used to monitor dynamic fission and fusion events.

Light beads microscopy is a two-photon microscopy approach that allows high-speed volumetric imaging of neuronal activity at the mesoscale.

An implantable array of LEDs called the Opto-Array facilitates optogenetic manipulations in the nonhuman primate brain.