Volume 17

  • No. 8 August 2020

    Red-shifted rhodamines for cellular imaging

    A photo of quartz cuvettes containing 5 µM solutions of the organic rhodamine dyes Janelia Fluor 479 (JF479), JF559, JF571 and JF593 excited at an angle with a 405-nm laser pointer.

    See Grimm et al.

  • No. 7 July 2020

    Multilayered scoring system selects sgRNAs

    The Vienna Bioactivity CRISPR (VBC) scoring system predicts sgRNAs that efficiently generate loss-of-function alleles. The artwork is inspired by the fairy tale of Cinderella, who receives help from pigeons to sort good and bad peas.

    See Michlits et al.

  • No. 6 June 2020

    Targeted sequencing perturbations

    The cover illustrates targeted Perturb-seq (TAP-seq), which uses a universal PCR handle and gene-specific primers to amplify genes of interest in single cells. TAP-seq boosts the scale and precision of functional genomics screens and uncovers enhancer–target gene interactions at genome scale.

    See Schraivogel et al.

  • No. 5 May 2020

    Screening for AAVs with desired tropisms

    Shown is a fluorescence image of mouse brain vasculature. Brain vasculature (cyan) can be genetically targeted with engineered adeno-associated viral capsid AAV-PHP. V1 here carries a fluorescent protein marker (magenta) after systemic injection into the mouse.

    See Kumar et al.

  • No. 4 April 2020

    Charge detection MS on an Orbitrap

    The cover art depicts the process of individual ion mass spectrometry (I2MS), which detects charge of single molecules from electrospray droplets to produce a spectral output directly into the mass domain.

    See Kafader et al.

  • No. 3 March 2020

    High-speed two-photon microscopy

    Reverberation microscopy image of in vivo mouse brain vasculature. Multiple independent planes at different depths are imaged simultaneously.

    See Beaulieu et al.

  • No. 2 February 2020

    Protein interaction fingerprinting using deep learning

    An artistic representation of molecular surface interaction fingerprinting (MaSIF). Information flows in living systems, such as when proteins interact with each other. The watercolor medium expresses that flow, which is further highlighted by the neural network, identifying their connection.

    See Gainza et al.

  • No. 1 January 2020


    Our choice for Method of the Year 2019 is single-cell multimodal omics analysis.