Volume 17 Issue 9, September 2020

Volume 17 Issue 9

In vivo Brillouin microscopy

The cover depicts a stimulated Brillouin scattering image of the pharynx and the reproductive system of Caenorhabditis elegans, artistically superimposed on a mesh model of the nematode.

See Remer et al.

Image: Shira Friedman. Cover design: Erin Dewalt.

Editorial

  • Editorial |

    We distributed an informal questionnaire to learn from scientists about their professional use of WeChat. We share some of their answers and discuss the multipurpose platform offered by WeChat.

This Month

  • This Month |

    A new way to see actin in action, hearing as others don’t, and saved by the guitar.

    • Vivien Marx
  • This Month |

    “I have no idea what’s awaiting me, or what will happen when this all ends. For the moment I know this: there are sick people and they need curing.” ―Albert Camus, The Plague

    • Katriona Shea
    • , Ottar N. Bjørnstad
    • , Martin Krzywinski
    •  & Naomi Altman

Correspondence

Research Highlights

  • Research Highlight |

    BRICseq provides access to brain-wide connectivity and can be combined with functional and transcriptomic studies for a comprehensive view of neuronal circuitry.

    • Nina Vogt
  • Research Highlight |

    Addition of a second constriction to CsgG-family nanopores improves the accuracy of homopolymer sequencing.

    • Arunima Singh

Technology Feature

  • Technology Feature |

    With advanced approaches to sample preparation and data analysis, scientists doing cryo-EM are starting to infer molecular movements from samples that are, literally, frozen.

    • Amber Dance

    Collection:

Review Articles

Brief Communications

  • Brief Communication |

    A spraying-mixing approach for preparing cryo-EM grids using the Spotiton robot allows time-resolved observations of short-lived biomolecular states.

    • Venkata P. Dandey
    • , William C. Budell
    • , Hui Wei
    • , Daija Bobe
    • , Kashyap Maruthi
    • , Mykhailo Kopylov
    • , Edward T. Eng
    • , Peter A. Kahn
    • , Jenny E. Hinshaw
    • , Nidhi Kundu
    • , Crina M. Nimigean
    • , Chen Fan
    • , Nattakan Sukomon
    • , Seth A. Darst
    • , Ruth M. Saecker
    • , James Chen
    • , Brandon Malone
    • , Clinton S. Potter
    •  & Bridget Carragher
  • Brief Communication |

    Repository-scale reanalysis of public mass spectrometry-based metabolomics data is facilitated by the Reanalysis of Data User (ReDU) interface, a system that uses consistent formatting and controlled vocabularies for metadata capture.

    • Alan K. Jarmusch
    • , Mingxun Wang
    • , Christine M. Aceves
    • , Rohit S. Advani
    • , Shaden Aguirre
    • , Alexander A. Aksenov
    • , Gajender Aleti
    • , Allegra T. Aron
    • , Anelize Bauermeister
    • , Sanjana Bolleddu
    • , Amina Bouslimani
    • , Andres Mauricio Caraballo Rodriguez
    • , Rama Chaar
    • , Roxana Coras
    • , Emmanuel O. Elijah
    • , Madeleine Ernst
    • , Julia M. Gauglitz
    • , Emily C. Gentry
    • , Makhai Husband
    • , Scott A. Jarmusch
    • , Kenneth L. Jones II
    • , Zdenek Kamenik
    • , Audrey Le Gouellec
    • , Aileen Lu
    • , Laura-Isobel McCall
    • , Kerry L. McPhail
    • , Michael J. Meehan
    • , Alexey V. Melnik
    • , Riya C. Menezes
    • , Yessica Alejandra Montoya Giraldo
    • , Ngoc Hung Nguyen
    • , Louis Felix Nothias
    • , Mélissa Nothias-Esposito
    • , Morgan Panitchpakdi
    • , Daniel Petras
    • , Robert A. Quinn
    • , Nicole Sikora
    • , Justin J. J. van der Hooft
    • , Fernando Vargas
    • , Alison Vrbanac
    • , Kelly C. Weldon
    • , Rob Knight
    • , Nuno Bandeira
    •  & Pieter C. Dorrestein
  • Brief Communication |

    Feature-based molecular networking allows the generation of molecular networks for mass spectrometry data that can recognize isomers, incorporate relative quantification and integrate ion mobility data.

    • Louis-Félix Nothias
    • , Daniel Petras
    • , Robin Schmid
    • , Kai Dührkop
    • , Johannes Rainer
    • , Abinesh Sarvepalli
    • , Ivan Protsyuk
    • , Madeleine Ernst
    • , Hiroshi Tsugawa
    • , Markus Fleischauer
    • , Fabian Aicheler
    • , Alexander A. Aksenov
    • , Oliver Alka
    • , Pierre-Marie Allard
    • , Aiko Barsch
    • , Xavier Cachet
    • , Andres Mauricio Caraballo-Rodriguez
    • , Ricardo R. Da Silva
    • , Tam Dang
    • , Neha Garg
    • , Julia M. Gauglitz
    • , Alexey Gurevich
    • , Giorgis Isaac
    • , Alan K. Jarmusch
    • , Zdeněk Kameník
    • , Kyo Bin Kang
    • , Nikolas Kessler
    • , Irina Koester
    • , Ansgar Korf
    • , Audrey Le Gouellec
    • , Marcus Ludwig
    • , Christian Martin H.
    • , Laura-Isobel McCall
    • , Jonathan McSayles
    • , Sven W. Meyer
    • , Hosein Mohimani
    • , Mustafa Morsy
    • , Oriane Moyne
    • , Steffen Neumann
    • , Heiko Neuweger
    • , Ngoc Hung Nguyen
    • , Melissa Nothias-Esposito
    • , Julien Paolini
    • , Vanessa V. Phelan
    • , Tomáš Pluskal
    • , Robert A. Quinn
    • , Simon Rogers
    • , Bindesh Shrestha
    • , Anupriya Tripathi
    • , Justin J. J. van der Hooft
    • , Fernando Vargas
    • , Kelly C. Weldon
    • , Michael Witting
    • , Heejung Yang
    • , Zheng Zhang
    • , Florian Zubeil
    • , Oliver Kohlbacher
    • , Sebastian Böcker
    • , Theodore Alexandrov
    • , Nuno Bandeira
    • , Mingxun Wang
    •  & Pieter C. Dorrestein
  • Brief Communication |

    Genetically encoded fluorescent-protein-tagged actin nanobodies fused to organelle membrane-targeting sequences enables high-resolution imaging of transient organelle–actin contact sites in live cells.

    • Cara R. Schiavon
    • , Tong Zhang
    • , Bing Zhao
    • , Andrew S. Moore
    • , Pauline Wales
    • , Leonardo R. Andrade
    • , Melissa Wu
    • , Tsung-Chang Sung
    • , Yelena Dayn
    • , Jasmine W. Feng
    • , Omar A. Quintero
    • , Gerald S. Shadel
    • , Robert Grosse
    •  & Uri Manor

Articles

  • Article |

    A method based on maximum likelihood density modification, adapted from X-ray crystallography, improves cryo-EM maps.

    • Thomas C. Terwilliger
    • , Steven J. Ludtke
    • , Randy J. Read
    • , Paul D. Adams
    •  & Pavel V. Afonine
  • Article |

    Chemically inducible trimerization tools based on split FRB or FKBP with full-length FKBP or FRB, respectively, expand the chemogenetics toolbox. Their efficiency and fast kinetics enable new types of protein manipulation in live cells.

    • Helen D. Wu
    • , Masaki Kikuchi
    • , Onur Dagliyan
    • , Adam K. Aragaki
    • , Hideki Nakamura
    • , Nikolay V. Dokholyan
    • , Takashi Umehara
    •  & Takanari Inoue
  • Article |

    Genetically encoded cysteine-rich tags enable formation of gold nanoparticles in situ for single-molecule imaging of individual proteins in the context of cellular ultrastructure in bacterial, yeast and mammalian cells.

    • Zhaodi Jiang
    • , Xiumei Jin
    • , Yuhua Li
    • , Sitong Liu
    • , Xiao-Man Liu
    • , Ying-Ying Wang
    • , Pei Zhao
    • , Xinbin Cai
    • , Ying Liu
    • , Yaqi Tang
    • , Xiaobin Sun
    • , Yan Liu
    • , Yanyong Hu
    • , Ming Li
    • , Gaihong Cai
    • , Xiangbing Qi
    • , She Chen
    • , Li-Lin Du
    •  & Wanzhong He

Amendments & Corrections

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