Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Volume 17 Issue 8, August 2020

Red-shifted rhodamines for cellular imaging

A photo of quartz cuvettes containing 5 µM solutions of the organic rhodamine dyes Janelia Fluor 479 (JF479), JF559, JF571 and JF593 excited at an angle with a 405-nm laser pointer.

See Grimm et al.

Image: Jonathan Grimm. Cover Design: Marina Spence.


  • We’ve made some recent updates to our various content type offerings at Nature Methods. Here is a cheat sheet.



Top of page ⤴

This Month

  • A platform for exploring GPCR secrets, powered by community, music and sports.

    • Vivien Marx
    This Month
Top of page ⤴


Top of page ⤴

Research Highlights

  • Direct imaging of carbohydrates using electrospray ion beam deposition and scanning tunneling microscopy.

    • Arunima Singh
    Research Highlight
  • Intravital three-dimensional bioprinting enables the biofabrication of constructs in a pre-existing 3D matrix, such as inside tissues of live animals.

    • Lei Tang
    Research Highlight
  • barRNAseq allows detailed explorations of signaling pathways that control embryonic differentiation.

    • Madhura Mukhopadhyay
    Research Highlight
  • RIC-seq enables in situ mapping of intra- and intermolecular RNA–RNA interactions.

    • Lin Tang
    Research Highlight
  • Tethering fluorescent proteins to ribosomes clears up background signals when imaging neural circuits.

    • Nina Vogt
    Research Highlight
Top of page ⤴

Technology Feature

Top of page ⤴


  • This Perspective highlights recently developed computational models for studying chromosome organization, with a focus on how mechanistic modeling helps biologists to interpret the biological function behind the genome structures.

    • Chris A. Brackey
    • Davide Marenduzzo
    • Nick Gilbert
Top of page ⤴


  • GPCRmd is a community-driven online platform to visualize, analyze and share G-protein-coupled receptor (GPCR) molecular dynamics data. It currently contains simulation data representing 100% of GPCR classes, 71% of receptor subtypes and 80% of GPCR families.

    • Ismael Rodríguez-Espigares
    • Mariona Torrens-Fontanals
    • Jana Selent
Top of page ⤴

Brief Communications

  • Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.

    • Sebastian Strauss
    • Ralf Jungmann
    Brief Communication
Top of page ⤴


Top of page ⤴

Amendments & Corrections

Top of page ⤴


Quick links