Volume 17 Issue 5, May 2020

Identifying things small and large in one mass spec experiment, and why persistence matters.

“Every day sadder and sadder news of its increase. In the City died this week 7496; and of them, 6102 of the plague. But it is feared that the true number of the dead this week is near 10,000 ....” —Samuel Pepys, 1665

A CRISPR–Cas9-based approach generates libraries of mutant proteins targeted to specific subcellular compartments for in situ directed evolution.

The assembly of polymers can be genetically targeted to specific neurons or other cells to manipulate their properties.

H-MAGMA leverages chromatin interaction data to interpret GWAS results for brain disorders.

A new platform allows researchers to study the tumor microenvironment in fully immunocompetent chimeric mice.

Researchers construct a transcriptome-based single-cell human cell landscape for major human organs.

To thwart SARS-CoV-2, labs hurry to hone assays, swap findings and share resources.

A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.

This Review highlights recent discoveries and applications of anti-CRISPR (Acr) proteins that enable the regulation of CRISPR-Cas technology.

This Resource describes genetic tools for microbial eukaryotes, providing a roadmap for developing genetically tractable organisms.

The meltome atlas compiles the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans, providing a resource for analyzing protein stability in the context of function and interactions.

‘Nativeomics’ enables identification of ligands bound to membrane proteins through detection of intact protein–ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment.

A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.

KAS-seq applies N₃-kethoxal to label guanines along single-stranded DNA in live cells, enabling characterization of ssDNA-involved transcription dynamics with as little as 1,000 cells.

Single-molecule displacement/diffusivity mapping (SMdM) enables nanoscale mapping of freely diffusing molecules in mammalian cells and reveals the structural basis of variations in local diffusivity in both the cytoplasm and nucleus.

In situ point spread function (PSF) retrieval (INSPR) enables precise single-molecule localization in 3D single-molecule localization microscopy of whole cells and tissues. It directly determines PSF from a single-molecule blinking dataset, removing errors associated with sample-induced aberrations.

M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism. The approach involves both positive and negative selection and yields vectors with diversified cell-type tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously.