Volume 17 Issue 5, May 2020

Volume 17 Issue 5

Screening for AAVs with desired tropisms

Shown is a fluorescence image of mouse brain vasculature. Brain vasculature (cyan) can be genetically targeted with engineered adeno-associated viral capsid AAV-PHP. V1 here carries a fluorescent protein marker (magenta) after systemic injection into the mouse.

See Kumar et al.

Image: Sripriya Ravindra Kumar. Cover Design: Erin Dewalt.

Editorial

  • Editorial |

    More basic research studies of marine microorganisms — supported by new methods, tools and resources — are needed to help inform policies to mitigate the impact of climate change.

This Month

  • This Month |

    Identifying things small and large in one mass spec experiment, and why persistence matters.

    • Vivien Marx
  • This Month |

    “Every day sadder and sadder news of its increase. In the City died this week 7496; and of them, 6102 of the plague. But it is feared that the true number of the dead this week is near 10,000 ....” —Samuel Pepys, 1665

    • Ottar N. Bjørnstad
    • , Katriona Shea
    • , Martin Krzywinski
    •  & Naomi Altman

Research Highlights

Technology Feature

News & Views

  • News & Views |

    A resource of detailed DNA delivery and expression protocols for marine protists will enable new studies to understand the fundamental and ancestral features of eukaryotic cells.

    • Matthew W. Brown
    •  & Alexander K. Tice

Review Articles

Resources

  • Resource | | Open Access

    This Resource describes genetic tools for microbial eukaryotes, providing a roadmap for developing genetically tractable organisms.

    • Drahomíra Faktorová
    • , R. Ellen R. Nisbet
    • , José A. Fernández Robledo
    • , Elena Casacuberta
    • , Lisa Sudek
    • , Andrew E. Allen
    • , Manuel Ares Jr
    • , Cristina Aresté
    • , Cecilia Balestreri
    • , Adrian C. Barbrook
    • , Patrick Beardslee
    • , Sara Bender
    • , David S. Booth
    • , François-Yves Bouget
    • , Chris Bowler
    • , Susana A. Breglia
    • , Colin Brownlee
    • , Gertraud Burger
    • , Heriberto Cerutti
    • , Rachele Cesaroni
    • , Miguel A. Chiurillo
    • , Thomas Clemente
    • , Duncan B. Coles
    • , Jackie L. Collier
    • , Elizabeth C. Cooney
    • , Kathryn Coyne
    • , Roberto Docampo
    • , Christopher L. Dupont
    • , Virginia Edgcomb
    • , Elin Einarsson
    • , Pía A. Elustondo
    • , Fernan Federici
    • , Veronica Freire-Beneitez
    • , Nastasia J. Freyria
    • , Kodai Fukuda
    • , Paulo A. García
    • , Peter R. Girguis
    • , Fatma Gomaa
    • , Sebastian G. Gornik
    • , Jian Guo
    • , Vladimír Hampl
    • , Yutaka Hanawa
    • , Esteban R. Haro-Contreras
    • , Elisabeth Hehenberger
    • , Andrea Highfield
    • , Yoshihisa Hirakawa
    • , Amanda Hopes
    • , Christopher J. Howe
    • , Ian Hu
    • , Jorge Ibañez
    • , Nicholas A. T. Irwin
    • , Yuu Ishii
    • , Natalia Ewa Janowicz
    • , Adam C. Jones
    • , Ambar Kachale
    • , Konomi Fujimura-Kamada
    • , Binnypreet Kaur
    • , Jonathan Z. Kaye
    • , Eleanna Kazana
    • , Patrick J. Keeling
    • , Nicole King
    • , Lawrence A. Klobutcher
    • , Noelia Lander
    • , Imen Lassadi
    • , Zhuhong Li
    • , Senjie Lin
    • , Jean-Claude Lozano
    • , Fulei Luan
    • , Shinichiro Maruyama
    • , Tamara Matute
    • , Cristina Miceli
    • , Jun Minagawa
    • , Mark Moosburner
    • , Sebastián R. Najle
    • , Deepak Nanjappa
    • , Isabel C. Nimmo
    • , Luke Noble
    • , Anna M. G. Novák Vanclová
    • , Mariusz Nowacki
    • , Isaac Nuñez
    • , Arnab Pain
    • , Angela Piersanti
    • , Sandra Pucciarelli
    • , Jan Pyrih
    • , Joshua S. Rest
    • , Mariana Rius
    • , Deborah Robertson
    • , Albane Ruaud
    • , Iñaki Ruiz-Trillo
    • , Monika A. Sigg
    • , Pamela A. Silver
    • , Claudio H. Slamovits
    • , G. Jason Smith
    • , Brittany N. Sprecher
    • , Rowena Stern
    • , Estienne C. Swart
    • , Anastasios D. Tsaousis
    • , Lev Tsypin
    • , Aaron Turkewitz
    • , Jernej Turnšek
    • , Matus Valach
    • , Valérie Vergé
    • , Peter von Dassow
    • , Tobias von der Haar
    • , Ross F. Waller
    • , Lu Wang
    • , Xiaoxue Wen
    • , Glen Wheeler
    • , April Woods
    • , Huan Zhang
    • , Thomas Mock
    • , Alexandra Z. Worden
    •  & Julius Lukeš
  • Resource |

    The meltome atlas compiles the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans, providing a resource for analyzing protein stability in the context of function and interactions.

    • Anna Jarzab
    • , Nils Kurzawa
    • , Thomas Hopf
    • , Matthias Moerch
    • , Jana Zecha
    • , Niels Leijten
    • , Yangyang Bian
    • , Eva Musiol
    • , Melanie Maschberger
    • , Gabriele Stoehr
    • , Isabelle Becher
    • , Charlotte Daly
    • , Patroklos Samaras
    • , Julia Mergner
    • , Britta Spanier
    • , Angel Angelov
    • , Thilo Werner
    • , Marcus Bantscheff
    • , Mathias Wilhelm
    • , Martin Klingenspor
    • , Simone Lemeer
    • , Wolfgang Liebl
    • , Hannes Hahne
    • , Mikhail M. Savitski
    •  & Bernhard Kuster

Brief Communications

  • Brief Communication |

    ‘Nativeomics’ enables identification of ligands bound to membrane proteins through detection of intact protein–ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment.

    • Joseph Gault
    • , Idlir Liko
    • , Michael Landreh
    • , Denis Shutin
    • , Jani Reddy Bolla
    • , Damien Jefferies
    • , Mark Agasid
    • , Hsin-Yung Yen
    • , Marcus J. G. W. Ladds
    • , David P. Lane
    • , Syma Khalid
    • , Christopher Mullen
    • , Philip M. Remes
    • , Romain Huguet
    • , Graeme McAlister
    • , Michael Goodwin
    • , Rosa Viner
    • , John E.P. Syka
    •  & Carol V. Robinson
  • Brief Communication |

    A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.

    • Alexandr Klioutchnikov
    • , Damian J. Wallace
    • , Michael H. Frosz
    • , Richard Zeltner
    • , Juergen Sawinski
    • , Verena Pawlak
    • , Kay-Michael Voit
    • , Philip St. J. Russell
    •  & Jason N. D. Kerr

Articles

Amendments & Corrections

  • Publisher Correction | | Open Access

    • Drahomíra Faktorová
    • , R. Ellen R. Nisbet
    • , José A. Fernández Robledo
    • , Elena Casacuberta
    • , Lisa Sudek
    • , Andrew E. Allen
    • , Manuel Ares Jr
    • , Cristina Aresté
    • , Cecilia Balestreri
    • , Adrian C. Barbrook
    • , Patrick Beardslee
    • , Sara Bender
    • , David S. Booth
    • , François-Yves Bouget
    • , Chris Bowler
    • , Susana A. Breglia
    • , Colin Brownlee
    • , Gertraud Burger
    • , Heriberto Cerutti
    • , Rachele Cesaroni
    • , Miguel A. Chiurillo
    • , Thomas Clemente
    • , Duncan B. Coles
    • , Jackie L. Collier
    • , Elizabeth C. Cooney
    • , Kathryn Coyne
    • , Roberto Docampo
    • , Christopher L. Dupont
    • , Virginia Edgcomb
    • , Elin Einarsson
    • , Pía A. Elustondo
    • , Fernan Federici
    • , Veronica Freire-Beneitez
    • , Nastasia J. Freyria
    • , Kodai Fukuda
    • , Paulo A. García
    • , Peter R. Girguis
    • , Fatma Gomaa
    • , Sebastian G. Gornik
    • , Jian Guo
    • , Vladimír Hampl
    • , Yutaka Hanawa
    • , Esteban R. Haro-Contreras
    • , Elisabeth Hehenberger
    • , Andrea Highfield
    • , Yoshihisa Hirakawa
    • , Amanda Hopes
    • , Christopher J. Howe
    • , Ian Hu
    • , Jorge Ibañez
    • , Nicholas A. T. Irwin
    • , Yuu Ishii
    • , Natalia Ewa Janowicz
    • , Adam C. Jones
    • , Ambar Kachale
    • , Konomi Fujimura-Kamada
    • , Binnypreet Kaur
    • , Jonathan Z. Kaye
    • , Eleanna Kazana
    • , Patrick J. Keeling
    • , Nicole King
    • , Lawrence A. Klobutcher
    • , Noelia Lander
    • , Imen Lassadi
    • , Zhuhong Li
    • , Senjie Lin
    • , Jean-Claude Lozano
    • , Fulei Luan
    • , Shinichiro Maruyama
    • , Tamara Matute
    • , Cristina Miceli
    • , Jun Minagawa
    • , Mark Moosburner
    • , Sebastián R. Najle
    • , Deepak Nanjappa
    • , Isabel C. Nimmo
    • , Luke Noble
    • , Anna M. G. Novák Vanclová
    • , Mariusz Nowacki
    • , Isaac Nuñez
    • , Arnab Pain
    • , Angela Piersanti
    • , Sandra Pucciarelli
    • , Jan Pyrih
    • , Joshua S. Rest
    • , Mariana Rius
    • , Deborah Robertson
    • , Albane Ruaud
    • , Iñaki Ruiz-Trillo
    • , Monika A. Sigg
    • , Pamela A. Silver
    • , Claudio H. Slamovits
    • , G. Jason Smith
    • , Brittany N. Sprecher
    • , Rowena Stern
    • , Estienne C. Swart
    • , Anastasios D. Tsaousis
    • , Lev Tsypin
    • , Aaron Turkewitz
    • , Jernej Turnšek
    • , Matus Valach
    • , Valérie Vergé
    • , Peter von Dassow
    • , Tobias von der Haar
    • , Ross F. Waller
    • , Lu Wang
    • , Xiaoxue Wen
    • , Glen Wheeler
    • , April Woods
    • , Huan Zhang
    • , Thomas Mock
    • , Alexandra Z. Worden
    •  & Julius Lukeš