Volume 16 Issue 3, March 2019

Volume 16 Issue 3

Vascularized kidney organoids

Kidney organoids cultured under flow create vascular networks that link to nearby organoids.

See Homan et al.

Image: Kimberly Homan and Navin Gupta. Cover design: Erin DeWalt

Editorial

  • Editorial |

    Software and algorithm development is crucial for scientific progress; we discuss how to improve the impact and recognition of these tools.

This Month

  • This Month |

    RNA-editing tools from a cricket-playing electrical engineer turned bioengineer.

    • Vivien Marx
  • This Month |

    Simultaneous examination of multiple factors at two levels can reveal which have an effect.

    • Byran Smucker
    • , Martin Krzywinski
    •  & Naomi Altman

Research Highlights

Technology Feature

Review Articles

  • Review Article |

    This Review discusses the strengths and limitations of methods to probe RNA–protein interactions.

    • Muthukumar Ramanathan
    • , Douglas F. Porter
    •  & Paul A. Khavari

Brief Communications

  • Brief Communication |

    The field synthesis method for generating any scanned light sheet is based on a new mathematical theorem in Fourier analysis and has important practical implications for simpler, multicolor lattice light-sheet microscopy.

    • Bo-Jui Chang
    • , Mark Kittisopikul
    • , Kevin M. Dean
    • , Philippe Roudot
    • , Erik S. Welf
    •  & Reto Fiolka
  • Brief Communication |

    RNA-guided adenosine deaminases edit point mutations in in vivo mouse models for Duchenne muscular dystrophy and ornithine transcarbamylase deficiency.

    • Dhruva Katrekar
    • , Genghao Chen
    • , Dario Meluzzi
    • , Ashwin Ganesh
    • , Atharv Worlikar
    • , Yu-Ru Shih
    • , Shyni Varghese
    •  & Prashant Mali
  • Brief Communication |

    FIt-SNE, a sped-up version of t-SNE, enables visualization of rare cell types in large datasets by obviating the need for downsampling. One-dimensional t-SNE heatmaps allow simultaneous visualization of expression patterns from thousands of genes.

    • George C. Linderman
    • , Manas Rachh
    • , Jeremy G. Hoskins
    • , Stefan Steinerberger
    •  & Yuval Kluger

Articles

  • Article |

    Cpf1-mediated genome editing of CAR-T cells enables efficient multiple targeting, knocking in chimeric antigen receptors while removing immune-checkpoint genes.

    • Xiaoyun Dai
    • , Jonathan J. Park
    • , Yaying Du
    • , Hyunu R. Kim
    • , Guangchuan Wang
    • , Youssef Errami
    •  & Sidi Chen
  • Article |

    Culturing human kidney organoids under fluidic shear conditions leads to robust vascularization and increased maturity. These kidney organoids should serve as a better model for kidney development than those developed in static culture.

    • Kimberly A. Homan
    • , Navin Gupta
    • , Katharina T. Kroll
    • , David B. Kolesky
    • , Mark Skylar-Scott
    • , Tomoya Miyoshi
    • , Donald Mau
    • , M. Todd Valerius
    • , Thomas Ferrante
    • , Joseph V. Bonventre
    • , Jennifer A. Lewis
    •  & Ryuji Morizane
  • Article |

    Acoustic scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.

    • Joon Ho Kang
    • , Teemu P. Miettinen
    • , Lynna Chen
    • , Selim Olcum
    • , Georgios Katsikis
    • , Patrick S. Doyle
    •  & Scott R. Manalis

Amendments & Corrections