Volume 16 Issue 3, March 2019

RNA-editing tools from a cricket-playing electrical engineer turned bioengineer.

Simultaneous examination of multiple factors at two levels can reveal which have an effect.

vDISCO combines tissue clearing with nanobody-based immunolabeling for reliable high-contrast imaging of the intact mouse.

The combination of image scanning microscopy and quantum imaging improves resolution up to fourfold compared with the classical diffraction barrier.

A search for more type V Cas12 family members turns up unexpected functionality.

A dual-barcoded shotgun expression library used in combination with DNA sequencing enables researchers to discover microbial functional traits.

Scientists exploit the basic physicochemical properties of protein–RNA complexes to paint a dynamic portrait of the RNA-binding proteome.

Labs combine techniques to study why cancer cells are so metabolically flexible.

This Review discusses the strengths and limitations of methods to probe RNA–protein interactions.

The field synthesis method for generating any scanned light sheet is based on a new mathematical theorem in Fourier analysis and has important practical implications for simpler, multicolor lattice light-sheet microscopy.

RNA-guided adenosine deaminases edit point mutations in in vivo mouse models for Duchenne muscular dystrophy and ornithine transcarbamylase deficiency.

FIt-SNE, a sped-up version of t-SNE, enables visualization of rare cell types in large datasets by obviating the need for downsampling. One-dimensional t-SNE heatmaps allow simultaneous visualization of expression patterns from thousands of genes.

Cpf1-mediated genome editing of CAR-T cells enables efficient multiple targeting, knocking in chimeric antigen receptors while removing immune-checkpoint genes.

Culturing human kidney organoids under fluidic shear conditions leads to robust vascularization and increased maturity. These kidney organoids should serve as a better model for kidney development than those developed in static culture.

Acoustic scattering in a suspended microchannel resonator can be used to measure mechanical properties of single cells in a noninvasive manner. The approach is applied to follow stiffness changes of individual cells throughout the cell cycle.