Volume 16 Issue 2, February 2019

Volume 16 Issue 2

Tracking animals in large populations

Artistic representation of the idtracker.ai method for tracking individual animals in large collectives using video analysis and convolutional network algorithms.

See Romero-Ferrero et al.

Image: Gil Costa, Champalimaud Center for the Unknown. Cover design: Erin Dewalt.

Editorial

  • Editorial |

    We shed some light on how the Nature Methods editorial team evaluates papers submitted to the journal.

This Month

  • This Month |

    How AI can help track animals and why breakfast powers collaboration.

    • Vivien Marx

Research Highlights

Technology Feature

Perspectives

  • Perspective |

    An interactive online resource integrated in the GPCRdb hub presents tools to design GPCR constructs and determine appropriate experimental conditions for structural studies by crystallography and cryo-EM.

    • Christian Munk
    • , Eshita Mutt
    • , Vignir Isberg
    • , Louise F. Nikolajsen
    • , Janne M. Bibbe
    • , Tilman Flock
    • , Michael A. Hanson
    • , Raymond C. Stevens
    • , Xavier Deupi
    •  & David E. Gloriam

Brief Communications

  • Brief Communication |

    NIR-GECO1, the first near-infrared genetically encoded calcium ion (Ca2+) indicator, enables improved Ca2+ imaging in conjunction with blue-light-activated optogenetic tools and multiplexed imaging in cell cultures and tissue slices.

    • Yong Qian
    • , Kiryl D. Piatkevich
    • , Benedict Mc Larney
    • , Ahmed S. Abdelfattah
    • , Sohum Mehta
    • , Mitchell H. Murdock
    • , Sven Gottschalk
    • , Rosana S. Molina
    • , Wei Zhang
    • , Yingche Chen
    • , Jiahui Wu
    • , Mikhail Drobizhev
    • , Thomas E. Hughes
    • , Jin Zhang
    • , Eric R. Schreiter
    • , Shy Shoham
    • , Daniel Razansky
    • , Edward S. Boyden
    •  & Robert E. Campbell
  • Brief Communication |

    A single-photon detector array enables robust and versatile image scanning microscopy (ISM) on any confocal microscope. This implementation makes super-resolution FLIM possible and eases a transition from confocal microscopy to ISM.

    • Marco Castello
    • , Giorgio Tortarolo
    • , Mauro Buttafava
    • , Takahiro Deguchi
    • , Federica Villa
    • , Sami Koho
    • , Luca Pesce
    • , Michele Oneto
    • , Simone Pelicci
    • , Luca Lanzanó
    • , Paolo Bianchini
    • , Colin J. R. Sheppard
    • , Alberto Diaspro
    • , Alberto Tosi
    •  & Giuseppe Vicidomini

Articles

  • Article |

    Trogocytosis, the uptake of membrane proteins by an antigen-presenting cell from its cognate T cell, allows the identification of neoepitopes targeted by T cell receptors with high sensitivity.

    • Guideng Li
    • , Michael T. Bethune
    • , Stephanie Wong
    • , Alok V. Joglekar
    • , Michael T. Leonard
    • , Jessica K. Wang
    • , Jocelyn T. Kim
    • , Donghui Cheng
    • , Songming Peng
    • , Jesse M. Zaretsky
    • , Yapeng Su
    • , Yicheng Luo
    • , James R. Heath
    • , Antoni Ribas
    • , Owen N. Witte
    •  & David Baltimore
  • Article |

    Engineered, bifunctional receptors present antigens and initiate signaling in response to binding to the cognate T cell receptor. Libraries built with SABRs can screen thousands of epitopes for the discovery of T cell target antigens.

    • Alok V. Joglekar
    • , Michael T. Leonard
    • , John D. Jeppson
    • , Margaret Swift
    • , Guideng Li
    • , Stephanie Wong
    • , Songming Peng
    • , Jesse M. Zaretsky
    • , James R. Heath
    • , Antoni Ribas
    • , Michael T. Bethune
    •  & David Baltimore

Amendments & Corrections