Volume 15 Issue 7, July 2018

A chemogenetic tool for biologists; how to be a scientist and a gardener.

Interferometric scattering microscopy enables quantitative mass imaging and biophysical characterization of single biomolecules in solution.

Aberration correction adds depth to light-sheet microscopy.

A combination of behavioral analysis and neural activity recording provides a glimpse into how the brain orchestrates complex behaviors.

Two new methods provide high-resolution profiles of splicing events in yeast.

3C-based methods and polymer modeling indentify the impact of structural variants.

Comparative methods are used to study vocalization in songbirds and bats.

Two methods for controlling protein activities with small molecules provide a general solution to a long-standing challenge in mammalian synthetic biology.

This Analysis compares and contrasts methods for measuring the mechanical properties of cells by applying the different approaches to the same breast cancer cell line.

Reanalysis of DNA-immunoprecipitation-based data shows that modification-specific antibodies bind unmodified short tandem repeats, and IgG controls are needed to avoid false positives.

A direct comparison of 5′-end RNA-seq methods reveals strong performance by CAGE, and identifies differential transcriptional start site usage among brain-related samples.

Off-targets identified by whole-genome sequencing of CRISPR-treated mouse embryos and their genetic parents are compared to predictions from GUIDE-seq and computational methods.

An automated method coupling tandem mass spectrometry with high-resolution mass spectrometry imaging simultaneously reveals both the molecular structure of lipids and their spatial locations in tissue.

Ligand-inducible connection (LInC) combines HCV protease and protease inhibitors to offer an orthogonal chemogenetic approach for controlling protein functions including protein localization, gene expression, and cell–cell communication.

Stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease enable robust, reversible, and orthogonal chemogenetic control of protein functions, including CRISPR–Cas9 activity, transcription, and protein dimerization.

EASI-tag, a new type of isobaric labeling reagents, enables multiplexed and highly accurate proteome quantification by mass spectrometry.

DeTiN can identify and account for signal from tumor cells that contaminate normal samples, for more sensitive somatic variant detection.

Fusion of a guide RNA directly to an adenosine deaminase via a SNAP-tag allows for precise and efficient RNA editing.

SAVER accurately recovers expression values in single-cell RNA-sequencing data to improve downstream analysis.

The GeNets web platform can identify the most informative network, as well as execute, store and share network-based analyses of RNA-seq or genomic datasets.

The ProximID approach generates single-cell expression profiles and a network of enriched physical cellular interactions within a tissue.