Latest Research

  • Brief Communication |

    A spraying-mixing approach for preparing cryo-EM grids using the Spotiton robot allows time-resolved observations of short-lived biomolecular states.

    • Venkata P. Dandey
    • , William C. Budell
    • , Hui Wei
    • , Daija Bobe
    • , Kashyap Maruthi
    • , Mykhailo Kopylov
    • , Edward T. Eng
    • , Peter A. Kahn
    • , Jenny E. Hinshaw
    • , Nidhi Kundu
    • , Crina M. Nimigean
    • , Chen Fan
    • , Nattakan Sukomon
    • , Seth A. Darst
    • , Ruth M. Saecker
    • , James Chen
    • , Brandon Malone
    • , Clinton S. Potter
    •  & Bridget Carragher
  • Brief Communication |

    Genetically encoded fluorescent-protein-tagged actin nanobodies fused to organelle membrane-targeting sequences enables high-resolution imaging of transient organelle–actin contact sites in live cells.

    • Cara R. Schiavon
    • , Tong Zhang
    • , Bing Zhao
    • , Andrew S. Moore
    • , Pauline Wales
    • , Leonardo R. Andrade
    • , Melissa Wu
    • , Tsung-Chang Sung
    • , Yelena Dayn
    • , Jasmine W. Feng
    • , Omar A. Quintero
    • , Gerald S. Shadel
    • , Robert Grosse
    •  & Uri Manor
  • Article |

    Genetically encoded cysteine-rich tags enable formation of gold nanoparticles in situ for single-molecule imaging of individual proteins in the context of cellular ultrastructure in bacterial, yeast and mammalian cells.

    • Zhaodi Jiang
    • , Xiumei Jin
    • , Yuhua Li
    • , Sitong Liu
    • , Xiao-Man Liu
    • , Ying-Ying Wang
    • , Pei Zhao
    • , Xinbin Cai
    • , Ying Liu
    • , Yaqi Tang
    • , Xiaobin Sun
    • , Yan Liu
    • , Yanyong Hu
    • , Ming Li
    • , Gaihong Cai
    • , Xiangbing Qi
    • , She Chen
    • , Li-Lin Du
    •  & Wanzhong He
  • Author Correction
    | Open Access

    • Wei Ouyang
    • , Casper F. Winsnes
    • , Martin Hjelmare
    • , Anthony J. Cesnik
    • , Lovisa Åkesson
    • , Hao Xu
    • , Devin P. Sullivan
    • , Shubin Dai
    • , Jun Lan
    • , Park Jinmo
    • , Shaikat M. Galib
    • , Christof Henkel
    • , Kevin Hwang
    • , Dmytro Poplavskiy
    • , Bojan Tunguz
    • , Russel D. Wolfinger
    • , Yinzheng Gu
    • , Chuanpeng Li
    • , Jinbin Xie
    • , Dmitry Buslov
    • , Sergei Fironov
    • , Alexander Kiselev
    • , Dmytro Panchenko
    • , Xuan Cao
    • , Runmin Wei
    • , Yuanhao Wu
    • , Xun Zhu
    • , Kuan-Lun Tseng
    • , Zhifeng Gao
    • , Cheng Ju
    • , Xiaohan Yi
    • , Hongdong Zheng
    • , Constantin Kappel
    •  & Emma Lundberg
  • Article |

    Chemically inducible trimerization tools based on split FRB or FKBP with full-length FKBP or FRB, respectively, expand the chemogenetics toolbox. Their efficiency and fast kinetics enable new types of protein manipulation in live cells.

    • Helen D. Wu
    • , Masaki Kikuchi
    • , Onur Dagliyan
    • , Adam K. Aragaki
    • , Hideki Nakamura
    • , Nikolay V. Dokholyan
    • , Takashi Umehara
    •  & Takanari Inoue
  • Article |

    Supervised machine-learning models trained using Drosophila epigenetic and STARR-seq data can be transferred to predict mouse and human enhancers.

    • Anurag Sethi
    • , Mengting Gu
    • , Emrah Gumusgoz
    • , Landon Chan
    • , Koon-Kiu Yan
    • , Joel Rozowsky
    • , Iros Barozzi
    • , Veena Afzal
    • , Jennifer A. Akiyama
    • , Ingrid Plajzer-Frick
    • , Chengfei Yan
    • , Catherine S. Novak
    • , Momoe Kato
    • , Tyler H. Garvin
    • , Quan Pham
    • , Anne Harrington
    • , Brandon J. Mannion
    • , Elizabeth A. Lee
    • , Yoko Fukuda-Yuzawa
    • , Axel Visel
    • , Diane E. Dickel
    • , Kevin Y. Yip
    • , Richard Sutton
    • , Len A. Pennacchio
    •  & Mark Gerstein
  • Article |

    A general tuning strategy is introduced for improving the utility of rhodamines for biological imaging applications. The strategy yielded bright, versatile and bioavailable far-red and near-infrared ‘Janelia Fluor’ dyes.

    • Jonathan B. Grimm
    • , Ariana N. Tkachuk
    • , Liangqi Xie
    • , Heejun Choi
    • , Boaz Mohar
    • , Natalie Falco
    • , Kathy Schaefer
    • , Ronak Patel
    • , Qinsi Zheng
    • , Zhe Liu
    • , Jennifer Lippincott-Schwartz
    • , Timothy A. Brown
    •  & Luke D. Lavis
  • Article |

    OligoFISSEQ combines Oligopaints with fluorescence in situ sequencing to enable the 3D mapping of many regions across the genome in human cells to interrogate genome organization at improved genomic resolution. OligoFISSEQ is compatible with immunochemistry and OligoSTORM for super-resolution imaging.

    • Huy Q. Nguyen
    • , Shyamtanu Chattoraj
    • , David Castillo
    • , Son C. Nguyen
    • , Guy Nir
    • , Antonios Lioutas
    • , Elliot A. Hershberg
    • , Nuno M. C. Martins
    • , Paul L. Reginato
    • , Mohammed Hannan
    • , Brian J. Beliveau
    • , George M. Church
    • , Evan R. Daugharthy
    • , Marc A. Marti-Renom
    •  & C.-ting Wu
  • Publisher Correction |

    • Ismael Rodríguez-Espigares
    • , Mariona Torrens-Fontanals
    • , Johanna K. S. Tiemann
    • , David Aranda-García
    • , Juan Manuel Ramírez-Anguita
    • , Tomasz Maciej Stepniewski
    • , Nathalie Worp
    • , Alejandro Varela-Rial
    • , Adrián Morales-Pastor
    • , Brian Medel-Lacruz
    • , Gáspár Pándy-Szekeres
    • , Eduardo Mayol
    • , Toni Giorgino
    • , Jens Carlsson
    • , Xavier Deupi
    • , Slawomir Filipek
    • , Marta Filizola
    • , José Carlos Gómez-Tamayo
    • , Angel Gonzalez
    • , Hugo Gutiérrez-de-Terán
    • , Mireia Jiménez-Rosés
    • , Willem Jespers
    • , Jon Kapla
    • , George Khelashvili
    • , Peter Kolb
    • , Dorota Latek
    • , Maria Marti-Solano
    • , Pierre Matricon
    • , Minos-Timotheos Matsoukas
    • , Przemyslaw Miszta
    • , Mireia Olivella
    • , Laura Perez-Benito
    • , Davide Provasi
    • , Santiago Ríos
    • , Iván R. Torrecillas
    • , Jessica Sallander
    • , Agnieszka Sztyler
    • , Silvana Vasile
    • , Harel Weinstein
    • , Ulrich Zachariae
    • , Peter W. Hildebrand
    • , Gianni De Fabritiis
    • , Ferran Sanz
    • , David E. Gloriam
    • , Arnau Cordomi
    • , Ramon Guixà-González
    •  & Jana Selent
  • Article |

    NanoLuc substrates with improved solubility and bioavailability, hydrofurimazine and fluorofurimazine, strongly enhance bioluminescence signals in vivo and enable bright dual-color bioluminescent imaging with AkaLuc and AkaLumine.

    • Yichi Su
    • , Joel R. Walker
    • , Yunhee Park
    • , Thomas P. Smith
    • , Lan Xiang Liu
    • , Mary P. Hall
    • , Louai Labanieh
    • , Robin Hurst
    • , David C. Wang
    • , Lance P. Encell
    • , Namdoo Kim
    • , Feijie Zhang
    • , Mark A. Kay
    • , Kerriann M. Casey
    • , Robbie G. Majzner
    • , Jennifer R. Cochran
    • , Crystal L. Mackall
    • , Thomas A. Kirkland
    •  & Michael Z. Lin
  • Resource |

    GPCRmd is a community-driven online platform to visualize, analyze and share G-protein-coupled receptor (GPCR) molecular dynamics data. It currently contains simulation data representing 100% of GPCR classes, 71% of receptor subtypes and 80% of GPCR families.

    • Ismael Rodríguez-Espigares
    • , Mariona Torrens-Fontanals
    • , Johanna K. S. Tiemann
    • , David Aranda-García
    • , Juan Manuel Ramírez-Anguita
    • , Tomasz Maciej Stepniewski
    • , Nathalie Worp
    • , Alejandro Varela-Rial
    • , Adrián Morales-Pastor
    • , Brian Medel-Lacruz
    • , Gáspár Pándy-Szekeres
    • , Eduardo Mayol
    • , Toni Giorgino
    • , Jens Carlsson
    • , Xavier Deupi
    • , Slawomir Filipek
    • , Marta Filizola
    • , José Carlos Gómez-Tamayo
    • , Angel Gonzalez
    • , Hugo Gutiérrez-de-Terán
    • , Mireia Jiménez-Rosés
    • , Willem Jespers
    • , Jon Kapla
    • , George Khelashvili
    • , Peter Kolb
    • , Dorota Latek
    • , Maria Marti-Solano
    • , Pierre Matricon
    • , Minos-Timotheos Matsoukas
    • , Przemyslaw Miszta
    • , Mireia Olivella
    • , Laura Perez-Benito
    • , Davide Provasi
    • , Santiago Ríos
    • , Iván R. Torrecillas
    • , Jessica Sallander
    • , Agnieszka Sztyler
    • , Silvana Vasile
    • , Harel Weinstein
    • , Ulrich Zachariae
    • , Peter W. Hildebrand
    • , Gianni De Fabritiis
    • , Ferran Sanz
    • , David E. Gloriam
    • , Arnau Cordomi
    • , Ramon Guixà-González
    •  & Jana Selent
  • Review Article |

    A review on recent method development for discovering T cell antigens.

    • Alok V. Joglekar
    •  & Guideng Li
  • Article |

    ZipSeq uses patterned illumination and photocaged oligonucleotides to serially print barcodes onto live, intact tissues for spatially mapping single cell transcriptomes.

    • Kenneth H. Hu
    • , John P. Eichorst
    • , Chris S. McGinnis
    • , David M. Patterson
    • , Eric D. Chow
    • , Kelly Kersten
    • , Stephen C. Jameson
    • , Zev J. Gartner
    • , Arjun A. Rao
    •  & Matthew F. Krummel
  • Article |

    The Ribosolve pipeline combines single-particle cryo-EM, M2-seq biochemical analysis and Rosetta auto-DRRAFTER modeling to guide three-dimensional RNA structure determination.

    • Kalli Kappel
    • , Kaiming Zhang
    • , Zhaoming Su
    • , Andrew M. Watkins
    • , Wipapat Kladwang
    • , Shanshan Li
    • , Grigore Pintilie
    • , Ved V. Topkar
    • , Ramya Rangan
    • , Ivan N. Zheludev
    • , Joseph D. Yesselman
    • , Wah Chiu
    •  & Rhiju Das
  • Brief Communication |

    Hundred-fold-faster DNA-PAINT imaging is enabled by the introduction of concatenated, periodic DNA sequence motifs in the docking strand. Six orthogonal sequences are described for speed-optimized and highly multiplexed cellular imaging.

    • Sebastian Strauss
    •  & Ralf Jungmann
  • Article |

    Small vibrational tags (azide, 13C-edited carbonyl and deuterium-labeled probes) were introduced as metabolic probes for mid-infrared imaging. The tags allow unprecedented in situ visualization of metabolism in cells and animals with high information throughput.

    • Lixue Shi
    • , Xinwen Liu
    • , Lingyan Shi
    • , H. Ted Stinson
    • , Jeremy Rowlette
    • , Lisa J. Kahl
    • , Christopher R. Evans
    • , Chaogu Zheng
    • , Lars E. P. Dietrich
    •  & Wei Min
  • Article |

    PULSE is an optogenetic tool that consists of two modules with different wavelength sensitivities. Their interplay enables optogenetic access to gene expression in plants independently of ambient light.

    • Rocio Ochoa-Fernandez
    • , Nikolaj B. Abel
    • , Franz-Georg Wieland
    • , Jenia Schlegel
    • , Leonie-Alexa Koch
    • , J. Benjamin Miller
    • , Raphael Engesser
    • , Giovanni Giuriani
    • , Simon M. Brandl
    • , Jens Timmer
    • , Wilfried Weber
    • , Thomas Ott
    • , Rüdiger Simon
    •  & Matias D. Zurbriggen
  • Brief Communication |

    CARPID uses CRISPR technology to navigate biotin ligase to specific lncRNAs, which allows proximal labeling and thus the querying of RNA–protein interactions in living cells.

    • Wenkai Yi
    • , Jingyu Li
    • , Xiaoxuan Zhu
    • , Xi Wang
    • , Ligang Fan
    • , Wenju Sun
    • , Linbu Liao
    • , Jilin Zhang
    • , Xiaoyu Li
    • , Jing Ye
    • , Fulin Chen
    • , Jussi Taipale
    • , Kui Ming Chan
    • , Liang Zhang
    •  & Jian Yan
  • Article |

    Multifocal flat illumination for field-independent imaging (mfFIFI) enables patterned illumination over an extended field of view. Integration with instant structured illumination microscope allowed for high-speed, multicolor, volumetric super-resolution imaging over 100 × 100 µm2.

    • Dora Mahecic
    • , Davide Gambarotto
    • , Kyle M. Douglass
    • , Denis Fortun
    • , Niccoló Banterle
    • , Khalid A. Ibrahim
    • , Maeva Le Guennec
    • , Pierre Gönczy
    • , Virginie Hamel
    • , Paul Guichard
    •  & Suliana Manley
  • Brief Communication |

    Split-FISH leverages a split-probe design to achieve enhanced specificity in multiplexed fluorescence in situ hybridization. Split-FISH reduces background and false positives, enabling transcriptome profiling in uncleared tissues.

    • Jolene Jie Lin Goh
    • , Nigel Chou
    • , Wan Yi Seow
    • , Norbert Ha
    • , Chung Pui Paul Cheng
    • , Yun-Ching Chang
    • , Ziqing Winston Zhao
    •  & Kok Hao Chen
  • Article |

    DeepSTORM3D uses deep learning for accurate localization of point emitters in densely labeled samples in three dimensions for volumetric localization microscopy with high temporal resolution, as well as for optimal point-spread function design.

    • Elias Nehme
    • , Daniel Freedman
    • , Racheli Gordon
    • , Boris Ferdman
    • , Lucien E. Weiss
    • , Onit Alalouf
    • , Tal Naor
    • , Reut Orange
    • , Tomer Michaeli
    •  & Yoav Shechtman
  • Perspective |

    This Perspective highlights recently developed computational models for studying chromosome organization, with a focus on how mechanistic modeling helps biologists to interpret the biological function behind the genome structures.

    • Chris A. Brackey
    • , Davide Marenduzzo
    •  & Nick Gilbert
  • Article |

    Guide RNA selection plays an essential role in CRISPR screens, the Vienna Bioactivity CRISPR (VBC) scoring system provides an improved selection for sgRNAs that generate loss-of-function alleles.

    • Georg Michlits
    • , Julian Jude
    • , Matthias Hinterndorfer
    • , Melanie de Almeida
    • , Gintautas Vainorius
    • , Maria Hubmann
    • , Tobias Neumann
    • , Alexander Schleiffer
    • , Thomas Rainer Burkard
    • , Michaela Fellner
    • , Max Gijsbertsen
    • , Anna Traunbauer
    • , Johannes Zuber
    •  & Ulrich Elling
  • Perspective |

    This Perspective reviews tools developed over the past five years in the macromolecular modeling, docking and design software Rosetta.

    • Julia Koehler Leman
    • , Brian D. Weitzner
    • , Steven M. Lewis
    • , Jared Adolf-Bryfogle
    • , Nawsad Alam
    • , Rebecca F. Alford
    • , Melanie Aprahamian
    • , David Baker
    • , Kyle A. Barlow
    • , Patrick Barth
    • , Benjamin Basanta
    • , Brian J. Bender
    • , Kristin Blacklock
    • , Jaume Bonet
    • , Scott E. Boyken
    • , Phil Bradley
    • , Chris Bystroff
    • , Patrick Conway
    • , Seth Cooper
    • , Bruno E. Correia
    • , Brian Coventry
    • , Rhiju Das
    • , René M. De Jong
    • , Frank DiMaio
    • , Lorna Dsilva
    • , Roland Dunbrack
    • , Alexander S. Ford
    • , Brandon Frenz
    • , Darwin Y. Fu
    • , Caleb Geniesse
    • , Lukasz Goldschmidt
    • , Ragul Gowthaman
    • , Jeffrey J. Gray
    • , Dominik Gront
    • , Sharon Guffy
    • , Scott Horowitz
    • , Po-Ssu Huang
    • , Thomas Huber
    • , Tim M. Jacobs
    • , Jeliazko R. Jeliazkov
    • , David K. Johnson
    • , Kalli Kappel
    • , John Karanicolas
    • , Hamed Khakzad
    • , Karen R. Khar
    • , Sagar D. Khare
    • , Firas Khatib
    • , Alisa Khramushin
    • , Indigo C. King
    • , Robert Kleffner
    • , Brian Koepnick
    • , Tanja Kortemme
    • , Georg Kuenze
    • , Brian Kuhlman
    • , Daisuke Kuroda
    • , Jason W. Labonte
    • , Jason K. Lai
    • , Gideon Lapidoth
    • , Andrew Leaver-Fay
    • , Steffen Lindert
    • , Thomas Linsky
    • , Nir London
    • , Joseph H. Lubin
    • , Sergey Lyskov
    • , Jack Maguire
    • , Lars Malmström
    • , Enrique Marcos
    • , Orly Marcu
    • , Nicholas A. Marze
    • , Jens Meiler
    • , Rocco Moretti
    • , Vikram Khipple Mulligan
    • , Santrupti Nerli
    • , Christoffer Norn
    • , Shane Ó’Conchúir
    • , Noah Ollikainen
    • , Sergey Ovchinnikov
    • , Michael S. Pacella
    • , Xingjie Pan
    • , Hahnbeom Park
    • , Ryan E. Pavlovicz
    • , Manasi Pethe
    • , Brian G. Pierce
    • , Kala Bharath Pilla
    • , Barak Raveh
    • , P. Douglas Renfrew
    • , Shourya S. Roy Burman
    • , Aliza Rubenstein
    • , Marion F. Sauer
    • , Andreas Scheck
    • , William Schief
    • , Ora Schueler-Furman
    • , Yuval Sedan
    • , Alexander M. Sevy
    • , Nikolaos G. Sgourakis
    • , Lei Shi
    • , Justin B. Siegel
    • , Daniel-Adriano Silva
    • , Shannon Smith
    • , Yifan Song
    • , Amelie Stein
    • , Maria Szegedy
    • , Frank D. Teets
    • , Summer B. Thyme
    • , Ray Yu-Ruei Wang
    • , Andrew Watkins
    • , Lior Zimmerman
    •  & Richard Bonneau
  • Article |

    Targeted sequencing of perturbation effects offers a sensitive approach to capture genes of interest in CRISPR-mediated screens, enabling genome-scale screens at higher scale and lower cost than whole-transcriptome Perturb-seq.

    • Daniel Schraivogel
    • , Andreas R. Gschwind
    • , Jennifer H. Milbank
    • , Daniel R. Leonce
    • , Petra Jakob
    • , Lukas Mathur
    • , Jan O. Korbel
    • , Christoph A. Merten
    • , Lars Velten
    •  & Lars M. Steinmetz
  • Article |

    Real-time 3D movement correction by tracking a fluorescent bead in the field of view enables functional imaging with 3D two-photon random-access microscopy in behaving mice and zebrafish.

    • Victoria A. Griffiths
    • , Antoine M. Valera
    • , Joanna YN. Lau
    • , Hana Roš
    • , Thomas J. Younts
    • , Bóris Marin
    • , Chiara Baragli
    • , Diccon Coyle
    • , Geoffrey J. Evans
    • , George Konstantinou
    • , Theo Koimtzis
    • , K. M. Naga Srinivas Nadella
    • , Sameer A. Punde
    • , Paul A. Kirkby
    • , Isaac H. Bianco
    •  & R. Angus Silver
  • Brief Communication |

    Sorting RT-FDC combines real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves to transfer molecular specificity to label-free, image-based cell sorting using an efficient deep neural network.

    • Ahmad Ahsan Nawaz
    • , Marta Urbanska
    • , Maik Herbig
    • , Martin Nötzel
    • , Martin Kräter
    • , Philipp Rosendahl
    • , Christoph Herold
    • , Nicole Toepfner
    • , Markéta Kubánková
    • , Ruchi Goswami
    • , Shada Abuhattum
    • , Felix Reichel
    • , Paul Müller
    • , Anna Taubenberger
    • , Salvatore Girardo
    • , Angela Jacobi
    •  & Jochen Guck
  • Brief Communication |

    Theoretical and experimental guidelines to determine laser intensity for single-photon excitation in microcrystals for ultrafast pump–probe experiments by serial femtosecond crystallography.

    • Marie Luise Grünbein
    • , Miriam Stricker
    • , Gabriela Nass Kovacs
    • , Marco Kloos
    • , R. Bruce Doak
    • , Robert L. Shoeman
    • , Jochen Reinstein
    • , Sylvain Lecler
    • , Stefan Haacke
    •  & Ilme Schlichting
  • Brief Communication |

    jYCaMP1, a yellow variant of the calcium indicator jGCaMP7, enables fast multicolor two-photon imaging at excitation wavelengths above 1,000 nm for use with popular ytterbium-doped fiber and modelocked semiconductor lasers.

    • Manuel Alexander Mohr
    • , Daniel Bushey
    • , Abhi Aggarwal
    • , Jonathan S. Marvin
    • , Jeong Jun Kim
    • , Emiliano Jimenez Marquez
    • , Yajie Liang
    • , Ronak Patel
    • , John J. Macklin
    • , Chi-Yu Lee
    • , Arthur Tsang
    • , Getahun Tsegaye
    • , Allison M. Ahrens
    • , Jerry L. Chen
    • , Douglas S. Kim
    • , Allan M. Wong
    • , Loren L. Looger
    • , Eric R. Schreiter
    •  & Kaspar Podgorski
  • Article |

    CRISPR-based microraft followed by guide RNA identification (CRaft-ID) combines microraft arrays, microscopy and CRISPR–Cas9 technology for high-content image-based phenotyping. CRaft-ID was used to identify proteins involved in stress granule formation.

    • Emily C. Wheeler
    • , Anthony Q. Vu
    • , Jaclyn M. Einstein
    • , Matthew DiSalvo
    • , Noorsher Ahmed
    • , Eric L. Van Nostrand
    • , Alexander A. Shishkin
    • , Wenhao Jin
    • , Nancy L. Allbritton
    •  & Gene W. Yeo
  • Brief Communication |

    A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.

    • Alexandr Klioutchnikov
    • , Damian J. Wallace
    • , Michael H. Frosz
    • , Richard Zeltner
    • , Juergen Sawinski
    • , Verena Pawlak
    • , Kay-Michael Voit
    • , Philip St. J. Russell
    •  & Jason N. D. Kerr
  • Article |

    In situ point spread function (PSF) retrieval (INSPR) enables precise single-molecule localization in 3D single-molecule localization microscopy of whole cells and tissues. It directly determines PSF from a single-molecule blinking dataset, removing errors associated with sample-induced aberrations.

    • Fan Xu
    • , Donghan Ma
    • , Kathryn P. MacPherson
    • , Sheng Liu
    • , Ye Bu
    • , Yu Wang
    • , Yu Tang
    • , Cheng Bi
    • , Tim Kwok
    • , Alexander A. Chubykin
    • , Peng Yin
    • , Sarah Calve
    • , Gary E. Landreth
    •  & Fang Huang
  • Brief Communication |

    ‘Nativeomics’ enables identification of ligands bound to membrane proteins through detection of intact protein–ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment.

    • Joseph Gault
    • , Idlir Liko
    • , Michael Landreh
    • , Denis Shutin
    • , Jani Reddy Bolla
    • , Damien Jefferies
    • , Mark Agasid
    • , Hsin-Yung Yen
    • , Marcus J. G. W. Ladds
    • , David P. Lane
    • , Syma Khalid
    • , Christopher Mullen
    • , Philip M. Remes
    • , Romain Huguet
    • , Graeme McAlister
    • , Michael Goodwin
    • , Rosa Viner
    • , John E.P. Syka
    •  & Carol V. Robinson
  • Brief Communication |

    Three-photon microscopy provides access to most of the adult zebrafish brain for both structural and functional imaging, as well as to the Danionella dracula brain.

    • Dawnis M. Chow
    • , David Sinefeld
    • , Kristine E. Kolkman
    • , Dimitre G. Ouzounov
    • , Najva Akbari
    • , Rose Tatarsky
    • , Andrew Bass
    • , Chris Xu
    •  & Joseph R. Fetcho
  • Article |

    M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism. The approach involves both positive and negative selection and yields vectors with diversified cell-type tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously.

    • Sripriya Ravindra Kumar
    • , Timothy F. Miles
    • , Xinhong Chen
    • , David Brown
    • , Tatyana Dobreva
    • , Qin Huang
    • , Xiaozhe Ding
    • , Yicheng Luo
    • , Pétur H. Einarsson
    • , Alon Greenbaum
    • , Min J. Jang
    • , Benjamin E. Deverman
    •  & Viviana Gradinaru