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Fluorescence microscopy today

Nature Methods volume 2, pages 902–904 (2005)Cite this article

Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear and hyper-resolution microscopies are poised to profoundly transform many fields of biological research.

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Acknowledgements

The author thanks M. Chalfie, F. Lanni, M. Nuriya and V. Nikolenko for comments and the Nuclear Energy Institute (NEI) and Howard Hughes Medical Institute (HHMI0 for support.

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  1. the Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, 10027, New York, USA

    Rafael Yuste

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Yuste, R. Fluorescence microscopy today. Nat Methods 2, 902–904 (2005). https://doi.org/10.1038/nmeth1205-902

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