Huttlin, E.L. et al. Nature 545, 505–509 (2017).

Most proteins do not act in isolation, but instead carry out their functions by interacting with other proteins in the cell. A key technology for identifying such interactions is affinity purification coupled with mass spectrometry (AP-MS), where a 'bait' protein is affinity-tagged, and, after pulldown, captured 'prey' proteins are identified by MS. In 2015, researchers presented BioPlex, a large-scale, systematic AP-MS analysis that detected nearly 24,000 interactions. Huttlin et al. now present BioPlex 2.0, a substantial further effort that reveals more than 56,000 interactions and covers more than 25% of human protein-coding genes. The authors additionally performed several biological analyses, identifying, for example, ∼1,300 protein communities that represent diverse cellular activities, and 442 communities associated with disease. The data and a graphical viewer are available to the community.