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Super-resolution Microscopy

In this collection:

Fluorescence microscopy is acquiring new capabilities as methodological developments allow it to break the diffraction limit—a barrier that until recently prevented light microscopy from being used to interrogate details of cellular function beyond the resolving power of conventional light microscopy. This collection of articles from several leaders in the field highlights the diversity of super-resolution microscopy techniques being developed and the principles that allow them to overcome this long-standing limitation.

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Editorial

Editorial

Microscopy begets nanoscopy

Daniel Evanko

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Super-resolution microscopy collection

Primer

Primer: fluorescence imaging under the diffraction limit

Daniel Evanko

Nature Methods 6, 19—20 (2009)

doi:10.1038/nmeth.f.235

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Perspective

Microscopy and its focal switch

Stefan W Hell

Nature Methods 6, 24—32 (2009)

doi:10.1038/nmeth.1291

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Commentary

Putting super-resolution fluorescence microscopy to work

Jennifer Lippincott-Schwartz & Suliana Manley

Nature Methods 6, 21—23 (2009)

doi:10.1038/nmeth.f.233

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Commentary

Subdiffraction resolution in continuous samples

Rainer Heintzmann & Mats G L Gustafsson

Nature Photonics 3, 362—364 (2009)

doi:10.1038/nphoton.2009.102

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News and Views

Single-molecule mountains yield nanoscale cell images

W E Moerner

Nature Methods 3, 781—782 (2006)

doi:10.1038/nmeth1006-781

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Article

Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

Hari Shroff, Catherine G Galbraith, James A Galbraith & Eric Betzig

Nature Methods 5, 417—423 (2008)

doi:10.1038/nmeth.1202

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Article

Spherical nanosized focal spot unravels the interior of cells

Roman Schmidt, Christian A Wurm, Stefan Jakobs, Johann Engelhardt, Alexander Egner & Stefan W Hell

Nature Methods 5, 539—544 (2008)

doi:10.1038/nmeth.1214

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Article

Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution

Bo Huang, Sara A Jones, Boerries Brandenburg & Xiaowei Zhuang

Nature Methods 5, 1047—1052 (2008)

doi:10.1038/nmeth.1274

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Brief Communication

Super-resolution video microscopy of live cells by structured illumination

Peter Kner, Bryant B Chhun, Eric R Griffis, Lukman Winoto & Mats G L Gustafsson

Nature Methods 6, 339—342 (2009)

doi:10.1038/nmeth.1324

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